Stability of the Virome in Lab- and Field-Collected Aedes albopictus Mosquitoes across Different Developmental Stages and Possible Core Viruses in the Publicly Available Virome Data of
            <i>Aedes</i>
            Mosquitoes

Shi, Chengying; Zhao, Lu; Atoni, Evans; Zeng, Weihua; Hu, Xiaomin; Matthijnssens, Jelle; Yuan, Zhiming; Xia, Han

doi:10.1128/msystems.00640-20

Cited by 57 publications

(107 citation statements)

References 59 publications

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“…Previous studies have shown that the viromes of the two important mosquitoes, A. aegypti and C. quinquefasciatus, have their own relatively stable core virome, which might have important implications for the competence to host the relevant arboviruses 17 . Moreover, further investigation revealed that the core virome was very stable across all developmental stages of both lab-derived and field-collected A. albopictus 35 . Our results suggested that different cryptic species of B. tabaci clearly harbor specific core virus/viruses (Fig.…”

Section: Discussionmentioning

confidence: 98%

“…3b), indicating a long-term coevolution between these ISVs and cryptic species of whitefly. Because the majority of the whitefly datasets used in this study are from public databases and are mostly derived from lab cultures, it is expected that the cryptic-species-specific core viruses in these whitefly cultures might constitute a vertically transmitted core virome, whereas the field whitefly dataset (FY-Q) likely harbors distinct environmentally-derived viruses (BtNeLV1-3, BtBeLV1-6, and BtBromoLV1-6) as described previously 35 . Nevertheless, more investigation is needed to confirm this hypothesis due to limited whitefly datasets from the field used in this study.…”

Section: Discussionmentioning

confidence: 99%

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Diversity and infectivity of the RNA virome among different cryptic species of an agriculturally important insect vector: whitefly Bemisia tabaci

Huang

Wang

et al. 2021

npj Biofilms Microbiomes

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A large number of insect-specific viruses (ISVs) have recently been discovered, mostly from hematophagous insect vectors because of their medical importance, but little attention has been paid to important plant virus vectors such as the whitefly Bemisia tabaci, which exists as a complex of cryptic species. Public SRA datasets of B. tabaci and newly generated transcriptomes of three Chinese populations are here comprehensively investigated to characterize the whitefly viromes of different cryptic species. Twenty novel ISVs were confidently identified, mostly associated with a particular cryptic species while different cryptic species harbored one or more core ISVs. Microinjection experiments showed that some ISVs might cross-infect between the two invasive whitefly cryptic species, Middle East Asia Minor 1 (MEAM1) and Mediterranean (MED), but others appeared to have a more restricted host range, reflecting the possibility of distinct long-term coevolution of these ISVs and whitefly hosts. Moreover, analysis of the profiles of virus-derived small-interfering RNAs indicated that some of the ISVs can successfully replicate in whitefly and the antiviral RNAi pathway of B. tabaci is actively involved in response to ISV infections. Our study provides a comprehensive analysis of the RNA virome, the distinct relationships and cross-cryptic species infectivity of ISVs in an agriculturally important insect vector.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Diversity and infectivity of the RNA virome among different cryptic species of an agriculturally important insect vector: whitefly Bemisia tabaci

Huang

Wang

et al. 2021

npj Biofilms Microbiomes

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“…These studies, which have primarily focused on analysis of viruses, have expanded our understanding of the breadth of viral diversity present in mosquito populations worldwide. More recently, similar approaches have been applied to examine the mosquito virome across different life stages of both lab-reared and wild-caught Aedes albopictus mosquitoes, providing intriguing insights to the potential stability and diversity of the mosquito virome ( Shi et al, 2020 ). Despite these insights, key epidemiologic information needed to direct interventions is still lacking.…”

Section: Introductionmentioning

confidence: 99%

Single mosquito metatranscriptomics identifies vectors, emerging pathogens and reservoirs in one assay

Batson

Dudas²,

Haas-Stapleton

et al. 2021

eLife

128

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Mosquitoes are major infectious disease-carrying vectors. Assessment of current and future risks associated with the mosquito population requires knowledge of the full repertoire of pathogens they carry, including novel viruses, as well as their blood meal sources. Unbiased metatranscriptomic sequencing of individual mosquitoes offers a straightforward, rapid, and quantitative means to acquire this information. Here, we profile 148 diverse wild-caught mosquitoes collected in California and detect sequences from eukaryotes, prokaryotes, 24 known and 46 novel viral species. Importantly, sequencing individuals greatly enhanced the value of the biological information obtained. It allowed us to (a) speciate host mosquito, (b) compute the prevalence of each microbe and recognize a high frequency of viral co-infections, (c) associate animal pathogens with specific blood meal sources, and (d) apply simple co-occurrence methods to recover previously undetected components of highly prevalent segmented viruses. In the context of emerging diseases, where knowledge about vectors, pathogens, and reservoirs is lacking, the approaches described here can provide actionable information for public health surveillance and intervention decisions.

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“…vpiRNAs can be determined by a ping-pong signature (U 1 -A 10 ) and 10 nt complementary overlapping reads. For the San Gabriel virus and all segments of Aedes phasma virus [ 58 , 146 ], as well as individual segments of TMTLV and WSBLV-4, we observed a ping-pong signature (U 1 -A 10 ) and over-represented complementary 10 nt overlapping pairs of sRNA from reads of 24–29 nt from these viruses ( Supplementary File S2 ). The absence of a predominant 21 nt species of vsiRNA targeting CppDNV and WSBLV-4 in Ae.…”

Section: Resultsmentioning

confidence: 99%

Uncovering the Worldwide Diversity and Evolution of the Virome of the Mosquitoes Aedes aegypti and Aedes albopictus

2021

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Aedes aegypti, the yellow fever mosquito, and Aedes albopictus, the Asian tiger mosquito, are the most significant vectors of dengue, Zika, and Chikungunya viruses globally. Studies examining host factors that control arbovirus transmission demonstrate that insect-specific viruses (ISVs) can modulate mosquitoes’ susceptibility to arbovirus infection in both in vivo and in vitro co-infection models. While research is ongoing to implicate individual ISVs as proviral or antiviral factors, we have a limited understanding of the composition and diversity of the Aedes virome. To address this gap, we used a meta-analysis approach to uncover virome diversity by analysing ~3000 available RNA sequencing libraries representing a worldwide geographic range for both mosquitoes. We identified ten novel viruses and previously characterised viruses, including mononegaviruses, orthomyxoviruses, negeviruses, and a novel bi-segmented negev-like group. Phylogenetic analysis suggests close relatedness to mosquito viruses implying likely insect host range except for one arbovirus, the multi-segmented Jingmen tick virus (Flaviviridae) in an Italian colony of Ae. albopictus. Individual mosquito transcriptomes revealed remarkable inter-host variation of ISVs within individuals from the same colony and heterogeneity between different laboratory strains. Additionally, we identified striking virus diversity in Wolbachia infected Aedes cell lines. This study expands our understanding of the virome of these important vectors. It provides a resource for further assessing the ecology, evolution, and interaction of ISVs with their mosquito hosts and the arboviruses they transmit.

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Stability of the Virome in Lab- and Field-Collected Aedes albopictus Mosquitoes across Different Developmental Stages and Possible Core Viruses in the Publicly Available Virome Data of Aedes Mosquitoes

Cited by 57 publications

References 59 publications

Diversity and infectivity of the RNA virome among different cryptic species of an agriculturally important insect vector: whitefly Bemisia tabaci

Diversity and infectivity of the RNA virome among different cryptic species of an agriculturally important insect vector: whitefly Bemisia tabaci

Single mosquito metatranscriptomics identifies vectors, emerging pathogens and reservoirs in one assay

Uncovering the Worldwide Diversity and Evolution of the Virome of the Mosquitoes Aedes aegypti and Aedes albopictus

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