Stability of urine specimens stored with and without preservatives at room temperature and on ice prior to urinalysis

Ercan, Müjgan; Akbulut, Emiş Deniz; Abuşoğlu, Sedat; Yılmaz, Fatma Meriç; Oğuz, Esra Fırat; Topçuoğlu, Canan; Öztekin, Volkan; Boğdaycıoğlu, Nihal

doi:10.1016/j.clinbiochem.2015.05.016

Cited by 26 publications

(18 citation statements)

References 8 publications

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“…Similarly, in a study by Ercan et al, changes in leukocyte morphology were detected, and negative effects of cold preservation on the crystal structure were observed [5]. Mostly, amorphous urate was found in the urine samples of our volunteers.…”

Section: Discussionmentioning

confidence: 74%

“…Use of stabilizers such as ethanol and polyethylene glycol may prevent metabolic changes and bacterial growth [3,5]. Urinalysis is important during primary care follow-up of pregnant women and is considered to be one of the most important criteria in the evaluation of healthcare systems' activities.…”

Section: Introductionmentioning

confidence: 99%

“…Prolongation of the period between the urine sample collection and the analysis (> 2 h), the inability to control the temperature, and an absence of preservative decrease the quality of the samples [3,4].As urine samples analysed at the Public Health Laboratory are transported by vehicle from family health centres (FHC) located far away, the period between obtaining the sample and the analysis may exceed 2 h. While transporting the samples in accordance with cold-chain principles may prevent bacterial growth and cell destruction or structural deterioration, but also negative effects may be observed on the crystal structure and other particles [1,5,6].…”

Section: Introductionmentioning

confidence: 99%

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The role of tubes with preservative in urinalysis of pregnant women

Avcı¹,

Aybek²,

Kangal³

et al. 2018

Med-Science

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In order to increase the efficiency of the preanalytical process at the Public Health Laboratory (PHL) where urinalysis is performed on pregnant women admitted at primary care family health centres (FHC), our aim was to demonstrate the efficacy of preservative urine tubes in this study. The first morning urine samples of 84 pregnant women were collected into urine tubes with or without preservative. The first analysis was used as a reference. The tubes with preservative containing chlorhexidine were transported at room temperature and analysed on the 2, 6, 24, and 72 h. Tubes with or without preservative and transported according to cold-chain principles were analysed on the 2nd and 6th h. Changes in comparison to the reference tube were analysed. When the test results of the tubes with preservative and kept at room temperature were compared to those of the reference tubes, the only changes that were observed were in the leukocyte morphology. During microscopic examination of the tubes with or without preservative and transported in accordance with cold-chain principles, no differences were found except pH changes during strip tests and changes in the crystal structure. The tubes with preservative containing chlorhexidine, whose performance was tested with the aim of controlling pre-analytical variables, may be used in conditions in which transportation in accordance with cold-chain principles is impossible. Tubes without preservative were observed to be adequate in conditions in which transportation in accordance with cold-chain principles is possible within 6 h.

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Section: Discussionmentioning

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The role of tubes with preservative in urinalysis of pregnant women

Avcı¹,

Aybek²,

Kangal³

et al. 2018

Med-Science

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“…Therefore, similar to feces, no standardized way of collecting and processing urine for storage in biobanks has been established yet. Generally, storing urine at room temperature for a maximum of 4 h is recommended before long-term storing at low temperatures [49,50]. A general protocol for urine collection and storage was published by the UK biobank [26,27].…”

Section: Urinementioning

confidence: 99%

Biobanking in Molecular Biomarker Research for the Early Detection of Cancer

Lommen

Odeh

Theije

et al. 2020

Cancers

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Although population-wide screening programs for several cancer types have been implemented in multiple countries, screening procedures are invasive, time-consuming and often perceived as a burden for patients. Molecular biomarkers measurable in non-invasively collected samples (liquid biopsies) could facilitate screening, as they could have incremental value on early diagnosis of cancer, but could also predict prognosis or monitor treatment response. Although the shift towards biomarkers from liquid biopsies for early cancer detection was initiated some time ago, there are many challenges that hamper the development of such biomarkers. One of these challenges is large-scale validation that requires large prospectively collected biobanks with liquid biopsies. Establishing those biobanks involves several considerations, such as standardization of sample collection, processing and storage within and between biobanks. In this perspective, we will elaborate on several issues that need to be contemplated in biobanking, both in general and for certain specimen types specifically, to be able to facilitate biomarker validation for early detection of cancer.

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“…Importantly, urine density, which is a useful measure of total urine (g/L), is based not only on the quantity, but also on the molecular mass of solute particles. [21][22][23] The USG can be modified by the presence of several sediment sources, including inorganic salt crystals, for example calcium phosphate and calcium oxalate, in the hours after sample collection and proteinuria. [24][25][26] A refractometer is an instrument that measures the concentration of a wide variety of aqueous solutions, such as biological matrices of urine and plasma, as well as fluids such as juices, milk, syrups, and other liquids derived from various industries.…”

Section: Introductionmentioning

confidence: 99%

Development of in‐house materials for the verification of specific gravity measurements: Homogeneity, stability, and proficiency studies

Velasco-Bejarano

Mercado

Camacho

et al. 2019

Drug Testing and Analysis

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We developed and evaluated the properties of in‐house urine reference materials for the verification of laboratory refractometers, which are frequently used in clinical chemistry and doping testing laboratories. Urine was gathered from 26 healthy volunteers (16 male 30 ± 5 years old and 10 female 29 ± 4 years old), from which two urine batches were obtained: one with a low specific gravity (1.012± 0.003) and the other with a high specific gravity (1.027 ± 0.003). Homogeneity studies were conducted over 20 consecutive days. For short‐term stability studies, aliquots of both urine batches were stored at −20 ± 2°C; 3 ± 2°C; 20 ± 2°C; 45 ± 2°C for 0, 2, 7, 14 and 35 days, under both light and dark conditions. Similarly, another study was conducted to measure the long‐term stability of urine at −20 ± 2°C, over a 24‐month evaluation period. Our data showed that the urine was homogeneous and stable at −20 ± 2°C, 3 ± 2°C, 20 ± 2°C, and 45 ± 2°C under both light and dark conditions. In all cases, the urine was evaluated by specific gravity and no statistically significant differences (p ≤ 0.05) were recorded. Additionally, a proficiency test was conducted in collaboration with 15 ISO/IEC 17025 accredited laboratories, and z‐scores and performance factors were evaluated. These data indicate that this material could be used for the verification of refractometers.

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Stability of urine specimens stored with and without preservatives at room temperature and on ice prior to urinalysis

Cited by 26 publications

References 8 publications

The role of tubes with preservative in urinalysis of pregnant women

The role of tubes with preservative in urinalysis of pregnant women

Biobanking in Molecular Biomarker Research for the Early Detection of Cancer

Development of in‐house materials for the verification of specific gravity measurements: Homogeneity, stability, and proficiency studies

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