Stability of viral haemorrhagic septicaemia virus, infectious hematopoietic necrosis virus and cyprinid herpesvirus 3 in various water samples

Abstract

In the recent decades, fish has become increasingly important for human nutrition. It is estimated that 30% of the food fish produced worldwide is attributable to aquaculture, with a rising trend to reach 50% in 2030 (Alonso & Leong, 2013). In Germany, aquaculture is the most productive sector of fish industry with a production volume of ca. 20,600 t of fish in 2017 with rainbow trout (Oncorhynchus mykiss) and common carp (Cyprinus carpio) being the most popular species and contributing together to about 65% of annual production (Brämick, 2017). Traditionally, many fish farms in Germany are rather small and often secondary and family-run activity. Nevertheless, an outbreak of a disease, resulting in high mortalities of the affected fish population, leads to losses that can challenge their economic survival independent from the size of the fish farm. The most important viruses that can infect common carp and rainbow trout in

Purification and concentration of infectious koi herpesvirus using steric exclusion chromatography

“…To our knowledge, no preparative purification strategies for infectious KHV have been published until now and only a few studies evaluated laboratory-scale purification (Bergmann et al, 2017). The achieved loss of less than 1 log TCID 50 mL −1 in this study is a positive result, considering the sensitivity of infectious KHV (Ullrich et al, 2021), and the set-point for further optimisations. However, several publications purified and concentrated KHV DNA from samples obtained from freshwater to investigate the spread of KHV into the environment (Haramoto et al, 2007(Haramoto et al, , 2009Honjo et al, 2010;Minamoto et al, 2009;Uchii et al, 2011).…”

From the basics to emerging diagnostic technologies: What is on the horizon for tilapia disease diagnostics?

“…206 eDNA assays, on the other hand, have indicated an antagonistic effect between bacterial loads and viral pathogens. 207,208 As aquaculture is an interactive complex system, 216,226 These technologies have potential to improve test sensitivity and enable quantitative testing. The use of readers also makes data digitalization, tracking, storage and transmission possible.…”

“…A synergistic association of bacterial microbiome and abundance of the parasitic ciliate Chilodonella hexasticha with mortality in barramundi ( Lates calcarifer ) has been demonstrated using a combination of metabarcoding‐ and targeting‐based approaches 206 . eDNA assays, on the other hand, have indicated an antagonistic effect between bacterial loads and viral pathogens 207,208 . As aquaculture is an interactive complex system, environmental parameters together with host and pathogen factors should be taken into account for eDNA/eRNA data analysis and interpretation.…”