Stability studies of immobilized lipase on rice husk and eggshell membrane

Abdulla, Rahmath; Sanny, S A; Derman, Eryati

doi:10.1088/1757-899x/206/1/012032

Cited by 14 publications

(4 citation statements)

References 21 publications

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“…Studies have also shown that the longitudinal tensile breaking force and thickness of the eggshell membrane of translucent eggs is lower than that of normal eggs, indicating that the membrane is more likely to break in translucent eggs, thus causing the water inside the egg to accumulate in the eggshell through the eggshell membrane to form translucent eggs [ 3 ]. Oil can protect the components of eggshell membranes, improve the oxidation resistance of eggshell membranes, and reduce the permeability of water [ 55 ]. Studies have shown that adding 1.85% mixed oil to the diet can significantly reduce the incidence of translucent eggs [ 56 ].…”

Section: Discussionmentioning

confidence: 99%

A Method to Reduce the Occurrence of Egg Translucency and Its Effect on Bacterial Invasion

Shi

Liang

Wang

et al. 2023

Foods

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Translucent egg consumption is low due to consumer acceptance and quality concerns, which is a problem that egg producers need to address. This study was performed to evaluate the reasons for the high occurrence of egg translucency in summer, as well as whether the addition of mono-dicalcium phosphate (MDCP) to the diet can relieve eggshell translucency and whether eggshell translucency is associated with the risk of bacterial invasion. A total of 72 laying hens that were 36 weeks old were randomly divided into control (CON) and MDCP groups and fed in the same environment. Results showed that the number of translucent eggs increases in July and August as the temperature and humidity increase. Compared with the CON group, in July, August, and October, the translucent egg grade (TEG) of the MDCP group was lower than that of the CON group (p < 0.05). TEG was correlated with mastoid space height (MSH), width (MSW), and area (MSA) (correlation coefficients 0.63, 0.59, and 0.68, respectively, p ≤ 0.05). There was no significant difference in the invasion rate of E. coli between translucent and non-translucent egg groups (47.2% vs. 39.33%), and translucent area and non-translucent area (13.49% vs. 15.08%). In conclusion, our results show that dietary MDCP may alleviate eggshell translucency and that eggshell translucency would not increase the probability of E. coli cross-shell penetration rate.

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Section: Discussionmentioning

confidence: 99%

A Method to Reduce the Occurrence of Egg Translucency and Its Effect on Bacterial Invasion

Shi

Liang

Wang

et al. 2023

Foods

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“…As evident from the data illustrated in Figure 1b, the enzyme loading was increased with the increasing amount of genipin concentration, where it reached a maximum value of 378.92 mg/g under 1.00% genipin. This was due to that the high genipin concentration reacts with the amino groups on the ESM surface, which increases the active sites of ESM and promotes the enzyme loading (Abdulla et al., 2017; Kessi & Arias, 2019). However, spatial hindrance of ESM increases with the increase of the enzyme load, resulting in the decrease of the unit enzyme activity (He et al., 2021).…”

Section: Resultsmentioning

confidence: 99%

“…Enzyme concentration, crosslinking temperature, and crosslinking time were all directly proportional to the enzyme loading (Abdulla et al., 2017; Wanjari et al., 2013; Zhuang et al., 2014). Therefore, the variations of Figure 1a,c, and d are similar to Figure 1b.…”

Section: Resultsmentioning

confidence: 99%

Evaluation on antiosteoporosis of collagen peptides prepared by immobilized protease with eggshell membrane

Zhang

et al. 2022

Journal of Food Science

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Collagen peptides are a potential treatment for osteoporosis due to their antiosteoporosis activity. In this study, we prepared immobilized protease with eggshell membrane as carrier, and then hydrolyzed collagen to obtain collagen peptide. The antiosteoporosis of collagen peptides was confirmed by hBMSC osteogenic differentiation and bone mineralization improvement results. Surprisingly, antiosteoporosis of collagen peptides was related to the molecular weight of collagen peptides. This was derived from the osteoblast marker gene expressions, and mineral elements in P1 treatment were higher than those in P3 treatment. Consequently, these results confirmed that antiosteoporosis of low molecular weight collagen peptides is higher than that of higher molecular weight collagen peptides. Furthermore, the antiosteoporosis activity of P1 was due to its peptide sequences with known antiosteoporosis activity in P1. Practical Application Using eggshell membrane as carrier to prepare immobilized protease was meaningful for solving the problem of resource waste. In addition, the results showed that collagen peptides possessed antiosteoporosis, and the effect of low molecular weight collagen peptides was better. This study provides a theoretical basis for developing high antiosteoporosis collagen peptides able to treat osteoporosis.

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“…Dissolution with acids also produces the eggshell membrane as a byproduct (Figure 4). Eggshell membrane is not only a cost-effective source of collagen and hyaluronic acid for the pharmaceutical and cosmetic industries [66,83], but unlike the calcified matrix, it has great potential to be used as a carrier for enzyme immobilization [65,72,[83][84][85][86]. This is supported by many studies focused on the development of biosensors based on the immobilization of enzymes on an eggshell membrane as solid carrier [73,83,[87][88][89][90][91][92][93][94][95][96].…”

Section: Carriers Based On Eggshell Membranesmentioning

confidence: 99%

Waste Management in the Agri-Food Industry: The Conversion of Eggshells, Spent Coffee Grounds, and Brown Onion Skins into Carriers for Lipase Immobilization

Budžaki

Velić

Ostojčić

et al. 2022

Foods

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One of the major challenges in sustainable waste management in the agri-food industry following the “zero waste” model is the application of the circular economy strategy, including the development of innovative waste utilization techniques. The conversion of agri-food waste into carriers for the immobilization of enzymes is one such technique. Replacing chemical catalysts with immobilized enzymes (i.e., immobilized/heterogeneous biocatalysts) could help reduce the energy efficiency and environmental sustainability problems of existing chemically catalysed processes. On the other hand, the economics of the process strongly depend on the price of the immobilized enzyme. The conversion of agricultural and food wastes into low-cost enzyme carriers could lead to the development of immobilized enzymes with desirable operating characteristics and subsequently lower the price of immobilized enzymes for use in biocatalytic production. In this context, this review provides insight into the possibilities of reusing food industry wastes, namely, eggshells, coffee grounds, and brown onion skins, as carriers for lipase immobilization.

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Stability studies of immobilized lipase on rice husk and eggshell membrane

Cited by 14 publications

References 21 publications

A Method to Reduce the Occurrence of Egg Translucency and Its Effect on Bacterial Invasion

A Method to Reduce the Occurrence of Egg Translucency and Its Effect on Bacterial Invasion

Evaluation on antiosteoporosis of collagen peptides prepared by immobilized protease with eggshell membrane

Waste Management in the Agri-Food Industry: The Conversion of Eggshells, Spent Coffee Grounds, and Brown Onion Skins into Carriers for Lipase Immobilization

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