Stability testing of resveratrol and viniferin obtained from Vitis vinifera L. by various extraction methods considering the industrial viewpoint

Abstract

Solid by-products generated in the winemaking process, can comprise valuable bioactive substances such as resveratrol and viniferin, which can be used in whole range of sectors including medicine, pharmacy, cosmetic industry etc. The changes in content of those stilbenes in extracts obtained by maceration and Soxhlet extraction were monitored using newly modified and validated highperformance liquid chromatography-mass spectrometry method which was proved to be accurate, reproducible, and efficient for their determination. The yields of individual bioactive compounds isolated from winery by-products are crucially dependent on the conditions of used extraction techniques. From this point of view, stability testing including light exposure, elevated temperature, and storage for longer time periods in the solution, represents the basis for optimizing conditions of extraction methods of resveratrol and trans-ε-viniferin. High temperature is beneficial for better release of thermally more stable stilbenes such as trans-resveratrol and trans-ε-viniferin but its application for prolonged time periods can be destructive. Light stress conditions cause the formation of otherwise unavailable cis-ε-viniferin by dimerization and photoisomerization of trans-stilbenes.

The Bark of the Spruce Picea jezoensis Is a Rich Source of Stilbenes

“…Different solvents, such as ethanol, methanol, or ethyl acetate, were previously applied to optimize stilbene extraction protocol [20,[29][30][31]. In this study, we compared the ability of different solvents, including methanol (MeOH), ethanol (EtOH), water (H 2 O), hexane, ethyl acetate, and acetone, to extract stilbenes from the bark of P. jezoensis.…”

“…Various organic solvents can be used for stilbene extraction and analysis. Although grapevine cane extracts are usually obtained using methanol or ethanol, there are also reports on using other solvents [29,30,42]. To select the most optimal solvent for stilbene extraction from the P. jezoensis bark, we used 100 mg of dry mass of crushed and dried bark collected in the winter and extracted stilbenes with methanol (100% and 70%), ethanol (96%, 70%, 40%), water, hexane, ethyl acetate, and acetone at 60 • C for 2 h. Determination of the most optimal temperature and time for stilbene extraction from the dried and crushed bark was carried out using methanol as a solvent at 20, 40, and 60 • C for 2, 4, and 6 h. After extraction, the extract was purified with Discovery ® DSC-18 SPE Tube bed wt.…”

Dermal Drug Delivery of Phytochemicals with Phenolic Structure via Lipid-Based Nanotechnologies

“…Since one or more of them are intrinsic for the majority of phenolic compounds, they do not always represent the best candidates for dermal transport [21,22]. Another limitation is often set by insufficient chemical stability of particular representatives [23,24] (e.g., resveratrol [25][26][27], hypericin [28,29], hyperforin [30][31][32], quercetin [33][34][35], cathehin [36][37][38]), for which precise control over the selection of dermal vehicles and technological operations for drug introduction in preformulation stage is required. It is worth mentioning that physicochemical properties, skin permeation, and chemical stability of phenolics are strongly affected by the presence of the glycoside part attached to the aglycone, and its type [39,40].…”

Impact of Climatic Conditions on the Resveratrol Concentration in Blend of Vitis vinifera L. cvs. Barbera and Croatina Grape Wines

“…Also, the production of wines containing high levels of resveratrol (>2 mg/L) depends on quality-oriented viticulture (suitable terroirs and sustainable viticulture practices) and winemaking technologies that prevent degradation of the compound [ 10 , 11 ]. Also, for the last 15 years, research has been conducted to study varieties of grapevine, wine, and grape juices using different extraction methods and solvents, to find a methodology leading to extracts with maximum yield of bioactive compounds, such as resveratrol [ 12 ].…”