Stabilization of phenylalanine ammonia lyase containing Rhodotorula glutinis cells for the continuous synthesis of l-phenylalanine methyl ester/96/

D’Cunha, Godwin B.; Satyanarayan, Vaduvatha; Nair, P.M.

doi:10.1016/s0141-0229(96)00013-0

Cited by 60 publications

(47 citation statements)

References 24 publications

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“…Phenylalanine ammonia-lyase (PAL) is the entry-point enzyme in the phenylpropanoid biosynthesis pathway and its presence has been demonstrated in all higher plants (Chen and McClure, 2000), some fungi and yeast (D'Cunha et al, 1996). Studies in several different species of plants have shown that the activity of PAL increases if they are subjected to biotic and abiotic factors, such as fungal infection (Kombrink and Somssich, 1995) and low temperature (Benkeblia, 2000;Jung et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Genetic determinants of the defense response of resistant and susceptible pepper (Capsicum annuum) cultivars infected with Phytophthora capsici (Oomycetes; Pythiaceae)

Zhang

Li²,

Gong

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Based on culture isolation and morphological observation blight-infected pepper plants in Shaanxi Province, China, we identified the pathogen causing pepper phytophthora blight as Phytophthora capsici. Varieties that differed in resistance (CM334, PBC602, and B27) were inoculated with this pathogen. The root activity of resistant CM334 variety was the highest while that of susceptible B27 variety was the lowest. Also, significant differences in the activity of POD, PAL, and β-1,3-glucanase were found; there was a positive correlation between disease resistance and activity of these three enzymes. We inhibited mycelial growth and sporangia formation of P. capsici using crude β-1,3-glucanase and PAL enzymes isolated from the resistant variety CM334 after it had been inoculated with P. capsici. These two enzymes had a synergistic effect on inhibition of P. capsici mycelial growth and sporangia formation. Expression of the defensive genes CaPO1, CaBGLU, CaBPR1, and CaRGA in the three varieties was higher in the leaves than in the roots. All three genes were upregulated in infected leaves and roots of the pepper plants, always expressing at higher levels in the resistant cultivar than in the susceptible cultivar, suggesting that the differences in resistance among the pepper genotypes involve differences in the timing and magnitude of the defense response.

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Section: Introductionmentioning

confidence: 99%

Genetic determinants of the defense response of resistant and susceptible pepper (Capsicum annuum) cultivars infected with Phytophthora capsici (Oomycetes; Pythiaceae)

Zhang

Li²,

Gong

et al. 2013

Genet. Mol. Res.

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“…PAL specific activities from wild-type R. toruloides were in the range of 10-15 U/g (DCW) [18,19]. Furthermore, the levels of PAL enzyme in these strains are relatively low.…”

Section: Discussionmentioning

confidence: 97%

“…Phenylalanine ammonia lyase (E.C.4.3.1.5-PAL) is widely distributed in higher plants, some fungi, yeasts and Streptomyces [1][2][3][4]. It has been used chiefly in the manufacture of L-phenylalanine by reversing the enzyme reaction with high concentration of transcinnamic acids and ammonia at an elevated pH.…”

Section: Introductionmentioning

confidence: 99%

“…The enzyme is therefore of particular interest to researchers in the biotechnological industry. At present, the industrial-scale production of PAL mainly utilizes the genus Rhodotorula [3]. The levels of enzyme in these wild-type strains are relatively low; thus, the production of L-phenylalanine from trans-cinnamic acids was of limited success.…”

Section: Introductionmentioning

confidence: 99%

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Optimal culture condition for the production of phenyalanine ammonia lyase from E. coli

Cui

2009

Korean J. Chem. Eng.

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The effects of culturing conditions on phenylalanine ammonia lyase production by a recombinant E. coli strain were investigated by using a controlled fed-batch fermentation system. In a 5 L fermentor, the optimal composition of the batch medium was 2% glucose, 1% yeast extract, 0.7% K 2 HPO 4 , 0.8% KH 2 PO 4 , 0.5% (NH 4 ) 2 SO 4 , 0.1% MgSO 4 ·7H 2 O. The optimal feed glucose solution was 50%. Glucose concentration and pH of the culture broth were maintained at about 2.0 g/L and 7.0 during the fed-batch phase, respectively. Following 24-h cultivation, 0.2 mmol/L isopropyl-β-D-thiogalactopyranoside (IPTG) was added and temperature was shifted from 37 o C to 42 o C to induce pal gene expression. Under optimal conditions, a high productivity of 300 U/g could be achieved after 48 h culture, and a cell density of OD 600 about 82 was obtained at 52 h culture at 500 r/m stirrer speed and 1 vvm, respectively.

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“…Phenylalanine ammonia lyase (e.c.4.3.1.5-PAl) is widely distributed in higher plants, some fungi, yeasts and Streptomyces (8,10,22,27). It is used chiefly in the manufacture of l-phenylalanine (l-Phe) by reversing the enzyme reaction with a high concentration of trans-cinnamic acids and ammonia at an elevated ph.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of Phenylalanine Ammonia Lyase Production fromRhodotorula Mucilaginosaby Optimization of Culture Conditions in Batch and Fed-Batch

Zhang

Cui

2012

Biotechnology & Biotechnological Equipment

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Stabilization of phenylalanine ammonia lyase containing Rhodotorula glutinis cells for the continuous synthesis of l-phenylalanine methyl ester/96/

Cited by 60 publications

References 24 publications

Genetic determinants of the defense response of resistant and susceptible pepper (Capsicum annuum) cultivars infected with Phytophthora capsici (Oomycetes; Pythiaceae)

Genetic determinants of the defense response of resistant and susceptible pepper (Capsicum annuum) cultivars infected with Phytophthora capsici (Oomycetes; Pythiaceae)

Optimal culture condition for the production of phenyalanine ammonia lyase from E. coli

Enhancement of Phenylalanine Ammonia Lyase Production fromRhodotorula Mucilaginosaby Optimization of Culture Conditions in Batch and Fed-Batch

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