Stabilization of planar lipid membranes: A stratified layer approach

Meier, Wolfgang; Graff, Alexandra; Diederich, Anke; Winterhalter, Mathias

doi:10.1039/b004073h

Cited by 50 publications

(39 citation statements)

References 14 publications

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“…The overall result is a "voltage overshoot" behavior. It agrees well with a previous report on the time dependent behavior of the membrane voltage by Meier et al [29]. As the external electric field is turned off beyond 4 us, the transmembrane potential falls dramatically with a time constant in the sub-microsecond range.…”

supporting

confidence: 82%

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Pulsed Electric Field Effects on Biological Cells

Schoenbach¹,

Beebe²,

Buescher³

et al. 2001

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Public reporting burden for this collection ot intormation is estimated to average yiour P* r sources, gathering and maintaining the data needed, and completing and reviewing trie collect aspect of this collection of information, including suggestions for reducing this burden, to Reports, 1215 Jefferson Davis Highway, Suite 1204, Arlington, VA 22202-4302, and to the Washington. DC 20503. AGENCY USE ONLY (Leave blank) AFRL-SR-BL-TR-02- ABSTRACT (Maximum 200 words) Jjc.A new type of field-cell interaction, "Intracellular Electromanipulation", by means of nonthermal, wideband electromagnetic radiation is being studied. It is based on capactive coupling to cell substructures, and has therfore the potential to affect transport processes across subcellular membrances. This was verified experimentally by applying electrical pulses of 60 nanosecond duration, with electric field amplitudes of 50 kV/cm to human eosinophils in vitro. Besides causing poration of intracellular membrances without disrupting the outer cell membrance, sub-microsecond pulses were found to induce apoptosis in cells. Reproduced AbstractA new type of field-cell interaction, "Intracellular Electromanipulation", by means of nonthermal, wideband electromagnetic radiation is being studied. It is based on capacitive coupling to cell substructures, and has therefore the potential to affect transport processes across subcellular membranes. This was verified experimentally by applying electrical pulses of 60 nanosecond duration, with electric field amplitudes of >50 kV/cm to human eosinophils in vitro. Besides causing poration of intracellular membranes without disrupting the outer cell membrane, sub-microsecond pulses were found to induce apoptosis in cells. This effect is being explored for cancer treatment. Experiments with sub-microsecond electric field exposure on a mouse model showed reduced tumor growth. In addition to wideband radiation effects, the response of cells to narrowband electromagnetic radiation in selected millimeter-wave (41 GHz -43 GHz), and radio-wave (800 kHz to 850 KHz) ranges was studied at moderate power levels (mW/cm 2 to W/cm 2 ). The results of experiments in both wavelength ranges did not indicate any resonance effects. A third field of bioelectric research, bacterial decontamination using nanosecond pulsed elctric fields and pulsed corona discharges in water, has been initiated. First results indicate increased decontamination efficiency with cold plasmas in water compared to pulsed electric fields. SummaryThe program in Basic and Applied Bioelectrics at Old Dominion University and Eastern Virginia Medical School, which was initiated and supported by this grant contains a medical/ biological and an environmental component. Research efforts have a) focused on the effect of high power, ultra-fast electrical pulses (wideband radiation) and that of moderate power, cw narrowband radiation on mammalian cells and tissues. b) Experiments on the effect of pulsed electric fields on bacteria either by direct irradiation or through el...

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supporting

confidence: 82%

“…This is borne out in our experimental measurements on E. coli, as discussed later. Also, this 0.1 ms time delay corresponds well with an experimental report by Meier et al [29]. Beyond 0.1 ms, the rate of pore reduction increases.…”

supporting

confidence: 73%

Pulsed Electric Field Effects on Biological Cells

Schoenbach¹,

Beebe²,

Buescher³

et al. 2001

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“…In this study, the stability of painted lipid bilayers was assessed according to a previously reported method (Meier et al 2000). The breaking point of bilayers was assessed by continuously increasing the electrical potential to a point where it breaks and a rapid increase in current is observed.…”

Section: Automated Formation Of Lipid Bilayers In Microporesmentioning

confidence: 99%

Polymer-based microfluidic device for measuring membrane protein activities

Kristiansen

2012

Microfluid Nanofluid

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Functional assays of membrane proteins are becoming increasingly important, both in research and drug discovery applications. The majority of current assays use the patch-clamp technology to measure the activity of ion channels which are over-expressed in cells. In future, in vitro assay systems will be available, which use reconstituted membrane proteins in free-standing lipid bilayers suspended in nano-or micrometer-sized pores. Such functional assays require (1) expression, purification and reconstitution of the membrane protein of interest, (2) a reliable method for lipid bilayer formation and membrane protein integration, and (3) a sensitive detection system. For practical applications, especially for automation, the reliable and controllable transport of fluids is essential. In order to achieve a stable free-standing lipid bilayer, a pore diameter in the micro-to nanometer range is essential. Novel microfluidic devices were developed by bonding a thick (300 lm) polyether ether ketone foil, bearing a channel structure, to a thin (12 lm) foil with a micropore of about 10 lm diameter and then utilized for the formation of stable, free-standing lipid bilayers within the pore. A bacterial voltage-gated potassium channel is integrated therein by fusion and the ion channel activity detected by voltage clamp.

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“…8 Recent progress has been made by stabilizing such membrane architectures either by crystallization of S-layer proteins or deposition of hydrogel cushions or other polymer layers. [9][10][11] The membrane lifetime could be enhanced to days; [12][13][14] however the resulting lifetime improvement remains insufficient to allow for continuous monitoring over periods of months, which may be a requirement for some applications.…”

Section: Introductionmentioning

confidence: 99%

Stable insulating tethered bilayer lipid membranes

et al. 2008

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Tethered bilayer lipid membranes have been shown to be an excellent model system for biological membranes. Coupling of a membrane to a solid supports creates a stable system that is accessible for various surface analytical tools. Good electrical sealing properties also enable the use of the membranes in practical sensing applications. The authors have shown that tethered membranes have extended lifetimes up to several months. Air-stability of the bilayer can be achieved by coating the membrane with a hydrogel. The structure of a monolayer and its stability under applied dc potentials have been investigated by neutron scattering. © 2008 American Vacuum Society.

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Stabilization of planar lipid membranes: A stratified layer approach

Cited by 50 publications

References 14 publications

Pulsed Electric Field Effects on Biological Cells

Pulsed Electric Field Effects on Biological Cells

Polymer-based microfluidic device for measuring membrane protein activities

Stable insulating tethered bilayer lipid membranes

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