Stabilized dried blood spot collection

McMorran, Darren; Chung, Dwayne Chung Kim; Tóth, Mónika; Liew, Oi Wah; Muradoglu, Murat; Ng, Tuck Wah

doi:10.1016/j.ab.2016.04.015

Cited by 7 publications

(3 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Consequently, this would mean the bioavailability of blood proteins from the HemaSpot™ is too low to provide valid results from ELISA. However, given that the overall proportion of sero-positive samples from the 2007 and 2010 Integrated Biological and Behavioural Surveillance Surveys [23] were approximately 11%, the results from the HemaSpot™ present a more conservative possibility of possible upward trend in HIV sero-positivity of key populations in Nigeria.…”

Section: Discussionmentioning

confidence: 93%

“…Studies have shown that major issues with laboratory results arise from the pre-analytic phase of analysis, which includes sample collection, and this is applicable to both collection devices [23]. The accuracy of a result can adversely be affected and/or invalidated if the sample is not collected properly, and the spot size varies as a result of (i) co-joined spots where two spots are applied too closely and merge together and (ii) smeared or splattered spots where blood is accidentally smeared before it is completely dried or splattered during spot application [23,24]. Sample collection devices designed to eliminate or limit these problems are needed.…”

Section: Discussionmentioning

confidence: 99%

“…Sample collection devices designed to eliminate or limit these problems are needed. McMorran et al (2016) [23] showed higher reliability and consistency of sample transfer to DBS cards when a stabilizing gyroscope was introduced into the sample collection technique.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Comparison of Dried Blood Spot Collection Techniques in Nigeria

Eluwa¹

2016

SOJI

View full text Add to dashboard Cite

SOJ Immunology Open Access Research Article prevalence rates for HIV among various groups such as women attending antenatal clinics, blood donors, transport workers and patients attending sexually transmitted infection (STI) clinics in the country. Such surveys are essential for providing evidence of the impact of HIV interventions, tracking trends of HIV prevalence and maintaining the efficacy of antiretroviral drug regimens within the population in areas where individualized patient monitoring is not available. A key aspect of these surveillance activities is the collection of bio-repository samples for use in secondary analysis as a means of quality control. The gold standard of specimen type for most surveys is plasma, however due to the increased cost and logistical challenges of separating and maintaining frozen plasma specimens, several studies investigated alternative specimen types for HIV antibody and antigen analysis [1]. In resourceconstrained settings with poor logistics and weak health system, alternate methods of specimen collection that do not require cold chain increase the efficiency of the health system as cost of sample transfer is reduced as well increasing the usage of limited resources within a defined area. Dried Blood Spots (DBS) are whole blood collected on filter paper and dried. DBS are used for re-testing at a reference laboratory, which is part of Nigeria's External Quality Assessment control in any survey carried out. Testing site results are then compared to reference laboratory results to ensure accuracy of results obtained from the field. DBS require minimal technical skills to collect and do not require cold-chain transportation [3] and therefore have been the most widely studied alternative specimen type [1,4-14]. With improvement in technology, DBS have produced results similar to those of plasma for quantitative viral load analysis [1,15-18] and HIV drug resistance genotyping analysis [4-14,19,20] for both patients on antiretroviral treatment and treatment-naive patients, indicating that DBS could be a viable alternative to plasma for HIV monitoring surveys [19]. Until recently, Whatman 903 (W-903) filter paper was the predominant filter paper used for DBS collection in studies analyzing DNA PCR for early infant diagnosis, HIV-1 loads [4,16,18] and serving as the only filter paper used for HIV drug Absract Background: Studies have focused on the type of filter paper to be used in sample collection for national surveys with optimum care in minimizing errors in field implementation among health personnel. Therefore, the aim of this study was to compare the HIV antigen/ antibody results of different dried blood spot collection devices. Methods: Dried blood spots were collected from key populations on both conventional DBS cards and HemaSpot™ which both used Munktell TFN (M-TFN) filter paper. Samples from the DBS cards were tested on HIV ELISA analysis using Vironostika 4th Generation ELISA assay. Bland-Altman analysis was used to measure limit level of agreement. Results: The ov...

show abstract

Section: Discussionmentioning

confidence: 93%