Stabilizing effects of calcium alginate microspheres on mycobacterium bovis BCG intended for oral vaccination

Dobakhti, Faramarz; Rahimi, Fatemeh; Dehpour, Ahmad Reza; Taghikhani, Mohammad; Ajdary, Soheila; Rafiei, Shahnaz; Rafiee-Tehrani, Morteza

doi:10.1080/02652040601034013

Cited by 16 publications

(8 citation statements)

References 35 publications

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“…However, there are several challenges to successful oral immunization due to the degradation of the antigens in the harsh environment of the gastrointestinal tract. Encapsulation of antigen protects it from enzymatic and chemical destruction of gastric secretions and thus can reduce the prescribed doses . Alginates as biodegradable and biocompatible polymers have been extensively used in biomedical fields such as cell encapsulation and controlled release delivery systems .…”

Section: Discussionmentioning

confidence: 99%

“…We have previously shown that BCG incorporated in alginate microspheres results in increased viability of bacilli in simulated gastric fluid in comparison with free BCG . Furthermore, intragastric administration of BCG encapsulated in alginate microspheres was shown to result in strong systemic protective immune responses against intravenous BCG challenge, 2 months after the immunization .…”

Section: Discussionmentioning

confidence: 99%

“…Bacille Calmette–Guerin was encapsulated in alginate microspheres through emulsification technique as described elsewhere . Briefly, fresh‐frozen Mycobacterium bovis BCG (Pasteur 1173‐P2 strain; the production complex of Pasteur Institute of Iran, Karaj, Iran) was added to an equal volume of sodium alginate (2% w/v; FMC biopolymer, Drammen, Norway) and mixed thoroughly.…”

Section: Methodsmentioning

confidence: 99%

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Immunization with Single Oral Dose of Alginate‐Encapsulated BCG Elicits Effective and Long‐Lasting Mucosal Immune Responses

et al. 2015

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Effective vaccination against pathogens, which enter the body through mucosal surfaces, requires the induction of both mucosal and systemic immune responses. Here, mucosal as well as systemic immune responses in the lung and spleen of BALB/c mice which were orally vaccinated with a single dose of alginateencapsulated bacille Calmette-Guerin (BCG) were evaluated. Twenty weeks after immunization, the vaccinated mice were challenged intranasally with BCG. Twelve weeks after immunization and 5 weeks after challenge, the immune responses were evaluated. Moreover, immune responses were compared with those of mice that were vaccinated with free BCG by subcutaneous (sc) and oral routes. Twelve weeks after the immunization, serum IgG level was higher in the sc-immunized mice, while serum IgA level was higher in the orally immunized mice with encapsulated BCG. Significant productions of both IgG and IgA were only detected in lungs of mice orally immunized with encapsulated BCG. Proliferative and delayed-type hypersensitivity responses and IFN-c production were significantly higher in mice immunized orally with encapsulated BCG, compared to mice immunized orally with free BCG. After challenge, the levels of IFN-c were comparable between sc-immunized mice with free BCG and orally immunized with encapsulated BCG; however, significantly less IL-4 was detected in mice which had received encapsulated BCG via oral route. Moreover, significant control of the bacilli growth in the lung of the immunized mice after intranasal challenge with BCG was documented in mice vaccinated with encapsulated BCG. These results suggest that oral immunization with alginateencapsulated BCG is an effective mean of inducing mucosal and systemic specific immune responses.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Immunization with Single Oral Dose of Alginate‐Encapsulated BCG Elicits Effective and Long‐Lasting Mucosal Immune Responses

et al. 2015

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“…This method of delivery has been investigated for the entrapment and oral delivery of live bacteria as probiotics (Lee and Heo, 2000) or as vaccines (Dobakhti et al, 2006). Although alginates have been shown as promising vehicles for oral delivery there are issues such as drug leaching, imbibing of acid, and competitive inhibition of carboxyl groups by some positively charged proteins affecting stability (Espevik et al, 1993).…”

Section: The Development Of Biopharmaceuticalsmentioning

confidence: 99%

Hydrocolloids and Medicinal Chemistry Applications

Grover

Smith

2009

Modern Biopolymer Science

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“…In this regard carriers such as chitosan/ alginate, etc have been employed with some degree of success [6,7] . BCG incorporated in alginate microspheres demonstrated an almost ninefold increase in viable bacilli in simulated gastric fluid (SGF) after 1.5 h, in comparison to free BCG, thereby demonstrating the potential of encapsulated BCG for oral vaccination [8] . In another study, alginateencapsulated BCG proved (reduced more colony forming units (CFU)) to be more protective in mice even after oral administration as compared to an un-encapsulated counterpart that was administered either orally or subcutaneously [9] .…”

Section: Tuberculosismentioning

confidence: 99%

Alginate-based sustained release drug delivery systems for tuberculosis

Ahmad

Khuller

2008

Expert Opinion on Drug Delivery

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Drug delivery systems have wide biomedical applications owing to their distinct therapeutic advantages, such as controlled release of drugs over prolonged periods, protection against premature drug degradation, reduction in drug toxicity and drug-drug interactions. All these factors are important considerations in the treatment of chronic infectious diseases such as tuberculosis. In tuberculosis, patient non-compliance is a vexing problem which is responsible not only for treatment failure, but also for the emergence of multi-drug resistant cases. Alginate, a natural polymer, has attracted researchers owing to its ease of availability, compatibility with hydrophobic as well as hydrophilic molecules, biodegradability under physiological conditions, lack of toxicity and the ability to confer sustained release potential. It is not therefore surprising that the controlled release phenomenon of this polymer has been documented for a vast array of drugs. In particular, the ability of alginate to co-encapsulate multiple antitubercular drugs and offer a controlled release profile is likely to have a major impact in enhancing patient compliance for better management of tuberculosis.

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Stabilizing effects of calcium alginate microspheres on mycobacterium bovis BCG intended for oral vaccination

Cited by 16 publications

References 35 publications

Immunization with Single Oral Dose of Alginate‐Encapsulated BCG Elicits Effective and Long‐Lasting Mucosal Immune Responses

Immunization with Single Oral Dose of Alginate‐Encapsulated BCG Elicits Effective and Long‐Lasting Mucosal Immune Responses

Hydrocolloids and Medicinal Chemistry Applications

Alginate-based sustained release drug delivery systems for tuberculosis

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