Stable anchoring of bacteria-based protein nanoparticles for surface enhanced cell guidance

Abstract

The preparation of robust nano–micropatterns for prolonged cell culture studies using covalent anchoring between the surface and protein nanoparticles, which are produced in a human-safe bacterial platform.

Recombinant vaccines in 2022: a perspective from the cell factory

“…However, major drawbacks include N-hyperglycosylation of proteins and low secretion efficiency. In fact, S. cerevisiae mannosylated glycoproteins are presumed to exhibit enhanced immunogenicity because they specifically interact with mannose-binding receptors found on professional antigen-presenting cells such as dendritic cells and macrophages [ 125 ].…”

“…As a result of its nonpathogenic nature and lack of toxins, S. cerevisiae is one of the yeast expression systems most exploited to obtain biopharmaceutical products [31,[121][122][123][124]. It is also considered generally safe (GRAS) by the Food and Drug Administration (FDA) and an accepted food additive by the European Food Safety Authority (EFSA) [125]. However, major drawbacks include N-hyperglycosylation of proteins and low secretion efficiency.…”

Protein scaffolds in human clinics

“…From a different point of view, bacterial inclusion bodies are selfassembling secretory amyloids in between the nano-and micro-scales (de Marco et al, 2019), that spontaneously formed in recombinant bacteria provide mechanical and biological stimuli when decorating surfaces for cell growth, motility and differentiation (Martinez-Miguel et al, 2020;Seras-Franzoso et al, 2015;Tatkiewicz et al, 2018). Inclusion bodies formed by growth factors such as the human FGF-2, the Mexican axolotl lipoxygenase and others offer a combination of mechanical and biological stimuli favoring among other effects, wound healing (Seras-Franzoso et al, 2014;Stamm et al, 2018).…”

Antibiofilm surfaces based on the immobilization of a novel recombinant antimicrobial multidomain protein using self-assembled monolayers

“…Moreover, for in depth characterization of the functionalized surfaces, prefunctionalized patterned and nonpatterned mixed polyethylene glycol (SH-PEG) and SH-PEG-NTA-Ni were prepared. Based on our previous expertise, to achieve spatial control of the distribution of the molecules 20 and pNPs, 21 the optimization of the microcontact printing (mCP) procedure of the NTA-terminated thiol (SH-PEG-NTA) and pegylated alkanethiol (SH-PEG) molecules was performed and a His-tagged green fluorescent protein (mGFPH6) as a reporter was used to optimize the procedure, easily visualizing the pattern. [22][23][24][25] Finally, an antibiofilm activity assay was carried out to evaluate the actual antimicrobial effect of the surfaces modified with the novel recombinant antimicrobial protein in its soluble and pNP forms.…”