Stable behavioral state-specific large scale activity patterns in the developing cortex of neonates

Mojtahedi, Nima; Kovalchuk, Yury; Böttcher, Alexander; Garaschuk, Olga

doi:10.1016/j.ceca.2021.102448

Cited by 4 publications

(3 citation statements)

References 73 publications

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“…At these stages, EEG is an unreliable tool for sleep scoring because early electrical signatures change rapidly across development and do not resemble adult patterns ( Blumberg et al, 2020 ). Using mesoscale calcium imaging while monitoring behavioral state, it has been shown that large events of spontaneous activity are confined to sleep periods by the end of the first postnatal week in mouse pups ( Mojtahedi et al, 2021 ; Tabuena et al, 2022 ). At earlier stages, the frequency of large events is independent of sleep–wake states.…”

Section: Recent Advances In Developmental Neuroscience Yielded By Mes...mentioning

confidence: 99%

“…However, they show a state-specific spatial preference whereby significantly more events are detected towards the somatosensory and motors cortices during periods of body movement. Conversely, activity is preferentially detected in the occipital regions during resting periods ( Mojtahedi et al, 2021 ; Tabuena et al, 2022 ). In sum, behavioral state influences the kind of activity observed in the cortex and, thus, it should be taken into account when conducting experiments using perinatal mice.…”

Section: Recent Advances In Developmental Neuroscience Yielded By Mes...mentioning

confidence: 99%

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Mesoscale calcium imaging in vivo: evolution and contribution to developmental neuroscience

Guillamón-Vivancos,

Vandael,

Torres

et al. 2023

Front. Neurosci.

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Calcium imaging is commonly used to visualize neural activity in vivo. In particular, mesoscale calcium imaging provides large fields of view, allowing for the simultaneous interrogation of neuron ensembles across the neuraxis. In the field of Developmental Neuroscience, mesoscopic imaging has recently yielded intriguing results that have shed new light on the ontogenesis of neural circuits from the first stages of life. We summarize here the technical approaches, basic notions for data analysis and the main findings provided by this technique in the last few years, with a focus on brain development in mouse models. As new tools develop to optimize calcium imaging in vivo, basic principles of neural development should be revised from a mesoscale perspective, that is, taking into account widespread activation of neuronal ensembles across the brain. In the future, combining mesoscale imaging of the dorsal surface of the brain with imaging of deep structures would ensure a more complete understanding of the construction of circuits. Moreover, the combination of mesoscale calcium imaging with other tools, like electrophysiology or high-resolution microscopy, will make up for the spatial and temporal limitations of this technique.

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Section: Recent Advances In Developmental Neuroscience Yielded By Mes...mentioning

confidence: 99%

Section: Recent Advances In Developmental Neuroscience Yielded By Mes...mentioning

confidence: 99%

Mesoscale calcium imaging in vivo: evolution and contribution to developmental neuroscience

Guillamón-Vivancos,

Vandael,

Torres

et al. 2023

Front. Neurosci.

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“…The neocortex of a P0 mouse is different from that of a P1 mouse, and that differs from a P2 mouse! In a careful and detailed study, one should either focus on one single postnatal day (Mojtahedi et al, 2021 ) or form groups that differ in age not more than 1–2 days (Yang et al, 2009 ; Shen and Colonnese, 2016 ).…”

Section: The Challenges Of Studying the Neurophysiology Of The Developing Cerebral Cortex In Rodentsmentioning

confidence: 99%

Neurophysiology of the Developing Cerebral Cortex: What We Have Learned and What We Need to Know

Luhmann

2022

Front. Cell. Neurosci.

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This review article aims to give a brief summary on the novel technologies, the challenges, our current understanding, and the open questions in the field of the neurophysiology of the developing cerebral cortex in rodents. In the past, in vitro electrophysiological and calcium imaging studies on single neurons provided important insights into the function of cellular and subcellular mechanism during early postnatal development. In the past decade, neuronal activity in large cortical networks was recorded in pre- and neonatal rodents in vivo by the use of novel high-density multi-electrode arrays and genetically encoded calcium indicators. These studies demonstrated a surprisingly rich repertoire of spontaneous cortical and subcortical activity patterns, which are currently not completely understood in their functional roles in early development and their impact on cortical maturation. Technological progress in targeted genetic manipulations, optogenetics, and chemogenetics now allow the experimental manipulation of specific neuronal cell types to elucidate the function of early (transient) cortical circuits and their role in the generation of spontaneous and sensory evoked cortical activity patterns. Large-scale interactions between different cortical areas and subcortical regions, characterization of developmental shifts from synchronized to desynchronized activity patterns, identification of transient circuits and hub neurons, role of electrical activity in the control of glial cell differentiation and function are future key tasks to gain further insights into the neurophysiology of the developing cerebral cortex.

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Large‐scale waves of activity in the neonatal mouse brain in vivo occur almost exclusively during sleep cycles

Tabuena

Huynh

Metcalf

et al. 2022

Developmental Neurobiology

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Spontaneous electrical activity plays major roles in the development of cortical circuitry. This activity can occur highly localized regions or can propagate over the entire cortex. Both types of activity coexist during early development. To investigate how different forms of spontaneous activity might be temporally segregated, we used wide‐field trans‐cranial calcium imaging over an entire hemisphere in P1–P8 mouse pups. We found that spontaneous waves of activity that propagate to cover the majority of the cortex (large‐scale waves; LSWs) are generated at the end of the first postnatal week, along with several other forms of more localized activity. We further found that LSWs are segregated into sleep cycles. In contrast, cortical activity during wake states is more spatially restricted and the few large‐scale forms of activity that occur during wake can be distinguished from LSWs in sleep based on their initiation in the motor cortex and their correlation with body movements. This change in functional cortical circuitry to a state that is permissive for large‐scale activity may temporally segregate different forms of activity during critical stages when activity‐dependent circuit development occurs over many spatial scales. Our data also suggest that LSWs in early development may be a functional precursor to slow sleep waves in the adult, which play critical roles in memory consolidation and synaptic rescaling.

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Stable behavioral state-specific large scale activity patterns in the developing cortex of neonates

Cited by 4 publications

References 73 publications

Mesoscale calcium imaging in vivo: evolution and contribution to developmental neuroscience

Mesoscale calcium imaging in vivo: evolution and contribution to developmental neuroscience

Neurophysiology of the Developing Cerebral Cortex: What We Have Learned and What We Need to Know

Large‐scale waves of activity in the neonatal mouse brain in vivo occur almost exclusively during sleep cycles

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