Stable Capillary Coating with Successive Multiple Ionic Polymer Layers

Katayama, Hiroyuki; Ishihama, Yasushi; Asakawa, Naoki

doi:10.1021/ac9708755

Cited by 202 publications

(201 citation statements)

References 36 publications

(47 reference statements)

Supporting

Mentioning

195

Contrasting

Unclassified

Order By: Relevance

“…The successive multiple-ionic-layer approach [60][61][62] for dynamic coating was applied to PDMS microfluidic devices by Liu et al [63]. In this method, polyelectrolyte multilayers are created by exposing a surface to alternating solutions of positively and negatively charged polyelectrolytes.…”

Section: Poly(dimethylsiloxane) Substratesmentioning

confidence: 99%

Surface modification in microchip electrophoresis

2003

View full text Add to dashboard Cite

Different approaches and techniques for surface modification of microfluidic devices applied for microchip electrophoresis are reviewed. The main focus is on the improved electrophoretic separation by reducing analyte-wall interactions and manipulation of electroosmosis. Approaches and methods for permanent and dynamic surface modification of microfluidic devices, manufactured from glass, quartz and also different polymeric substrates, are described.

show abstract

Section: Poly(dimethylsiloxane) Substratesmentioning

confidence: 99%

Surface modification in microchip electrophoresis

2003

View full text Add to dashboard Cite

show abstract

“…Keywords Capillary electrophoresis, dynamic coating, hydroxyethyl cellulose, polyethylene glycol, humic acid, DNA ladder Notes layers technique 19 is suitable for used in alkaline conditions, but the technique is not suitable for eliminating EOF. We present here a dynamic coating-based CE separation method for water soluble humic substances that improves operability, repeatability, and durability by employing an aqueous solution of mixed non-ionic polymers of different types and molecular weight as both the dynamic coating agent and the separation medium.…”

Section: Introductionmentioning

confidence: 99%

Dynamic Coating Capillary Electrophoresis for Separation of Humic Acid Using Mixture Solution of Non-ionic Polymers both as Coating Agent and Separation Medium

et al. 2013

View full text Add to dashboard Cite

A simple, rapid, and precise dynamic coating capillary electrophoretic separation method for water-soluble humic substances is proposed. An aqueous solution containing hydroxyethyl cellulose (HEC), polyethylene glycol (PEG), and pH buffer component was employed for both the dynamic coating agent and the separation medium. The procedure for the coating of the capillary inner wall was simply filling the buffered polymer mixture solution into the capillary that had been treated with 1 M aqueous HC1 solution. The solution for the capillary coating was directly used as the electrophoretic buffer solution for CE separation. Excellent performance for the separation of humic acid was obtained using the solution containing 0.5% (w/v) HEC, 1.0% (w/v) PEG 10000, and 0.1% (w/v) PEG 8000000. Excellent reproducibility and durability were obtained even at slightly alkaline conditions at pH levels above 8. The separation of 0.1 - 2 kbp of DNA ladder by the proposed method showed was also achieved

show abstract

“…In addition, the analytes are more stable and also possess strong intrinsic fluorescence characteristics at low pH when excited by using a UV laser at 266 nm [7,8]. In order to increase EOF mobility at low pH, strategies such as use of CTAB as well as anionic and cationic polymers to form bilayers or multilayers on the capillary wall have been suggested [12][13][14][15][16]. Alternately, the separation of biogenic amines and their metabolites by CE in conjunction with LIF detection has been conducted under discontinuous conditions using a capillary treated with 0.5 M NaOH prior to each run [8].…”

Section: Introductionmentioning

confidence: 99%

Capillary electrophoretic separation of biologically active amines and acids using nanoparticle‐coated capillaries

et al. 2008

View full text Add to dashboard Cite

This manuscript describes dynamic coating of capillaries with poly(L-lysine) (PLL) and silica nanoparticles (SiO 2 NPs) and use of the as-prepared capillaries for the separation of biogenic amines and acids by CE in conjunction with LIF detection. The directions of EOF are controlled by varying the outmost layer of the capillaries with PLL and SiO 2 NPs, respectively. Over the pH range 3.0-5.0, the (PLL-SiO 2 NP) n -PLL capillaries have an EOF toward the anodic end and are more suitable for the separation of acids with respect to speed, while the (PLL-SiO 2 NP) n capillaries have an EOF toward the cathodic end and are more suitable for the separation of biogenic amines regarding speed and sensitivity. The separations of standard solutions containing five amines and two acids by CE with LIF detection using (PLL-SiO 2 NP) 2 -PLL and (PLL-SiO 2 NP) 3 capillaries were accomplished within 10 and 7 min, providing plate numbers of 3.8 and 5.0610 4 plates/m for 5-hydroxytryptamine (5-HT), respectively. The LODs for 5-HT and 5-hydroxyindole-3-acetic acid (5-HIAA) are 32 and 2 nM and 0.2 and 1.5 nM when using the (PLL-SiO 2 NP) 2 -PLL and (PLL-SiO 2 NP) 3 capillaries, respectively. Identification and quantification of 5-HIAA, homovanillic acid, and DL-vanillomandelic acid in urine samples from a male before and after drinking green tea were tested to validate practicality of the present approach. The results show that the (PLLSiO 2 NP) 2 -PLL capillary provides greater resolving power, while the (PLL-SiO 2 NP) 3 capillary provides better sensitivity, higher efficiency, and longer durability for the separation of the amines and acids.

show abstract

Stable Capillary Coating with Successive Multiple Ionic Polymer Layers

Cited by 202 publications

References 36 publications

Surface modification in microchip electrophoresis

Surface modification in microchip electrophoresis

Dynamic Coating Capillary Electrophoresis for Separation of Humic Acid Using Mixture Solution of Non-ionic Polymers both as Coating Agent and Separation Medium

Capillary electrophoretic separation of biologically active amines and acids using nanoparticle‐coated capillaries

Contact Info

Product

Resources

About