Stable isotope compounds - production, detection, and application

Zachleder, Vilém; Vítová, Milada; Hlavová, Monika; Moudříková, Šárka; Mojzeš, Peter; Heumann, Hermann; Becher, Johannes Robert; Bišová, Kateřina

doi:10.1016/j.biotechadv.2018.01.010

Cited by 45 publications

(34 citation statements)

References 219 publications

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“…Deuterium‐labeled compounds are widely used as internal standards for spectroscopy and for biochemical applications . Replacing carbon–hydrogen bonds by more stable carbon–deuterium bonds may have a positive effect on metabolic stability while retaining the potency and selectivity of the compound . As a consequence, deuterated drugs have been intensively studied .…”

Section: Methodsmentioning

confidence: 99%

Catalytic C₁ Alkylation with Methanol and Isotope‐Labeled Methanol

et al. 2018

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Section: Methodsmentioning

confidence: 99%

Catalytic C₁ Alkylation with Methanol and Isotope‐Labeled Methanol

et al. 2018

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“…Because of the largest difference in the relative atomic mass, the stable isotope of hydrogen exhibits the strongest isotopic effect compared to other stable isotopes. The isotopic effect refers to the influence of an isotope on the vibrational frequency of diatomic bonds [38]. Hence, the vibrational frequency of the molecular C H or C D stretching is significantly affected in terms of band shift to lower wavenumbers due to the deuteriation, meaning the substitution of hydrogen by deuterium in biochemical processes [33,37].…”

Section: Stable Isotope Labelingmentioning

confidence: 99%

Imaging the invisible—Bioorthogonal Raman probes for imaging of cells and tissues

Matanfack

Rüger

Stiebing

et al. 2020

Journal of Biophotonics

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A revolutionary avenue for vibrational imaging with super‐multiplexing capability can be seen in the recent development of Raman‐active bioortogonal tags or labels. These tags and isotopic labels represent groups of chemically inert and small modifications, which can be introduced to any biomolecule of interest and then supplied to single cells or entire organisms. Recent developments in the field of spontaneous Raman spectroscopy and stimulated Raman spectroscopy in combination with targeted imaging of biomolecules within living systems are the main focus of this review. After having introduced common strategies for bioorthogonal labeling, we present applications thereof for profiling of resistance patterns in bacterial cells, investigations of pharmaceutical drug‐cell interactions in eukaryotic cells and cancer diagnosis in whole tissue samples. Ultimately, this approach proves to be a flexible and robust tool for in vivo imaging on several length scales and provides comparable information as fluorescence‐based imaging without the need of bulky fluorescent tags.

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“…The extent of stable isotope labeling by natural substance is low and this phenomenon is commonly used as a marker in metabolites. [29] However, these low amounts are not beneficial for metabolic analysis, which then leads to the use of isotope enriched samples. Although metabolic isotope enrichment is conceptually an easy approach, practically it faces a number of problems.…”

Section: Stable-isotope Labelingmentioning

confidence: 99%

Cancer Cell Metabolites: Updates on Current Tracing Methods

Maniam

2020

ChemBioChem

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Cancer is the second leading cause of death‐1 in 6 deaths globally is due to cancer. Cancer metabolism is a complex and one of the most actively researched area in cancer biology. Metabolic reprogramming in cancer cells entails activities that involve several enzymes and metabolites to convert nutrient into building blocks that alter energy metabolism to fuel rapid cell division. Metabolic dependencies in cancer generate signature metabolites that have key regulatory roles in tumorigenesis. In this minireview, we highlight recent advances in the popular methods ingrained in biochemistry research such as stable and flux isotope analysis, as well as radioisotope labeling, which are valuable in elucidating cancer metabolites. These methods together with analytical tools such as chromatography, nuclear magnetic resonance spectroscopy and mass spectrometry have helped to bring about exploratory work in understanding the role of important as well as obscure metabolites in cancer cells. Information obtained from these analyses significantly contribute in the diagnosis and prognosis of tumors leading to potential therapeutic targets for cancer therapy.

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Stable isotope compounds - production, detection, and application

Cited by 45 publications

References 219 publications

Catalytic C₁ Alkylation with Methanol and Isotope‐Labeled Methanol

Catalytic C₁ Alkylation with Methanol and Isotope‐Labeled Methanol

Imaging the invisible—Bioorthogonal Raman probes for imaging of cells and tissues

Cancer Cell Metabolites: Updates on Current Tracing Methods

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Stable isotope compounds - production, detection, and application

Cited by 45 publications

References 219 publications

Catalytic C1 Alkylation with Methanol and Isotope‐Labeled Methanol

Catalytic C1 Alkylation with Methanol and Isotope‐Labeled Methanol

Imaging the invisible—Bioorthogonal Raman probes for imaging of cells and tissues

Cancer Cell Metabolites: Updates on Current Tracing Methods

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Product

Resources

About

Catalytic C₁ Alkylation with Methanol and Isotope‐Labeled Methanol

Catalytic C₁ Alkylation with Methanol and Isotope‐Labeled Methanol