Stable Lentiviral Vector Transfer into Mesenchymal Stem Cells In Vivo

Abstract

Green fluorescent protein (eGFP) gene was transferred into mouse mesenchymal stem cells in vivo using a lentiviral vector. In 2 months after injection of the lentivirus into the cavity of the femoral bone, up to 30% fibroblast CFU in the bone marrow of infected mice contained the alien gene. The transferred gene was found in more than 50% of adherent layers of longterm bone marrow cultures formed by mesenchymal stem cells from the infected mice bone marrow; 4% fibroblast CFU obtained from these layers were labeled. Ectopic hemopoiesis foci developed after transplantation of the bone marrow from infected mice under the renal capsule of syngeneic recipients contained bone tissue labeled with the alien gene in 57% cases and labeled fibroblast CFU in 11%. The data confirm the possibility of gene transfer with the lentiviral vectors into the mesenchymal stem cells in vivo.

Lentivirus-mediated CDglyTK gene-modified free flaps by intra-artery perfusion show targeted therapeutic efficacy in rat model of breast cancer

“…Compared to other vectors, lentivectors have the potential for a greater infection efficiency and more stable host genome integration of the target gene, leading to longer therapeutic gene expression. In fact, a transferred gene was continuously expressed in mesenchymal stem cells for 2 months after injection of lentivirus-mediated enhanced GFP into the cavity of mouse femoral bone in vivo [22, 23]. The tumor inhibition effect of neural stem cells transfected with a CD/TK fusion gene using lentivectors lasted 21 days in a glioma mouse model [24], and furthermore, lentivectors have been successfully used in clinical trials for the correction of adrenoleukodystrophy, β-thalassemia, and leukemia [25].…”

hTERT-Immortalized Bone Mesenchymal Stromal Cells Expressing Rat Galanin via a Single Tetracycline-Inducible Lentivirus System

“…Lentiviral vectors have been used for the stable integration and long-term expression of transgenes. Lentiviral vectors are also favorable for biological research and gene therapy trials due to their ability to infect both dividing and nondividing cells and are particularly suitable for BMSCs [27]. Thus, in the present study, using a lentiviral system, we introduced the immortalization gene, hTERT, into primary rat BMSCs.…”