Stable Maintenance of Plasmid CLO DF13: Structural and Functional Relationships Between Replication Control, Partitioning, and Incompatibility

Plasmid incompatibility.

“…Sometimes the replication of the affected plasmid is completely blocked; more often the block is partial, or even minimal, and it is proposed that in such cases loss of the plasmid is due to inability to correct fluctuations arising as a consequence of the random temporal distribution of replication events. Also, vectorial incompatibility may result from interference with partitioning (56, 61) and has been observed with cloned fragments of unknown function (20,53). It is noted parenthetically that, with unit copy plasmids such as F and P1, it is impossible to analyze coresident incompatible plasmids simply because the copy numbers are too low to permit the construction of heteroplasmid strains.…”

“…See, e.g., Nijkamp et al (53), Gryczan et al (20), and Meyer et al (43). These have not been characterized and could involve Cmp-like phenomena (20,53).…”

Plasmid Incompatibility

“…Sometimes the replication of the affected plasmid is completely blocked; more often the block is partial, or even minimal, and it is proposed that in such cases loss of the plasmid is due to inability to correct fluctuations arising as a consequence of the random temporal distribution of replication events. Also, vectorial incompatibility may result from interference with partitioning (56, 61) and has been observed with cloned fragments of unknown function (20,53). It is noted parenthetically that, with unit copy plasmids such as F and P1, it is impossible to analyze coresident incompatible plasmids simply because the copy numbers are too low to permit the construction of heteroplasmid strains.…”

“…See, e.g., Nijkamp et al (53), Gryczan et al (20), and Meyer et al (43). These have not been characterized and could involve Cmp-like phenomena (20,53).…”