Stage-specific cuticular proteins of Ostertagia circumcincta and Ostertagia ostertagi

Keith, Karen; Duncan, Mary C.; Murray, M.; Bairden, K.; Tait, Andrew

doi:10.1016/0020-7519(90)90047-q

Cited by 15 publications

(3 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, developmental variation in the glycoprotein content of surface structures has been demonstrated for Ostertagia spp. (33), and for several other nematodes including H. contortus (30,34), and Oesophagostomum dentatum 28 . In this respect, it may be relevant that in our earlier work we have shown that IgE responses to T. circumcincta were predominantly to the L3 infective larvae, with only low responses detected against the adult worms (9).…”

Section: Discussionmentioning

confidence: 99%

Studies on the immunoglobulin E responses to Teladorsagia circumcincta in sheep: purification of a major high molecular weight allergen

Huntley

Redmond

Welfare

et al. 2001

Parasite Immunology

View full text Add to dashboard Cite

Studies on the immunoglobulin (Ig)E immune responses to the gastric nematode, Teladorsagia circumcincta, have demonstrated a major high molecular weight allergen (HMWTc). Cross reactive allergens of similar MW were demonstrated for Trichostrongylus colubriformis and Cooperia curticei, but not for Haemonchus contortus. Purification of HMWTc was achieved by gel-filtration chromatography, and nonreducing SDS-PAGE and Western blot analysis revealed two closely associated bands with a molecular weight of approximately 140-150 kDa. Reduction showed four IgE reactive bands of 120, 50, 45 and 30 kDa, and deglycosylation abrogated the immunoreactivity of the 120 and 30 kDa bands. Ultrastructural immunolocalization by electron microscopy revealed that the IgE reactivity was confined to the cuticular surface of the infective (L3) larvae. ELISA studies to determine the IgE anti-HMWTc responses in lambs during their first grazing season, demonstrated significantly higher IgE antibody in lambs with low accumulative faecal egg count (FEC) compared to animals with high accumulative FEC. These studies provide evidence for a protective function of IgE antibody in Teladorsagia infections in lambs.

show abstract

Section: Discussionmentioning

confidence: 99%

Studies on the immunoglobulin E responses to Teladorsagia circumcincta in sheep: purification of a major high molecular weight allergen

Huntley

Redmond

Welfare

et al. 2001

Parasite Immunology

View full text Add to dashboard Cite

show abstract

“…The pellet was resuspended in 500 µl TBS containing 1 % SDS and boiled for 5 min. The mixture was than recentrifuged for 30 min at 16 000 g, the supernatant was removed (AP2 fraction) and the pellet was resuspended in 500 µl TBS with 5 % 2-mercaptoethanol to obtain AP3 fraction (Keith et al, 1990;Wędrychowicz et al, 1994). The extracts were frozen at -20°C.…”

Section: Isolation Of Surface Proteins Of S Obvelatamentioning

confidence: 99%

The presence of Syphacia obvelata in laboratory mice (BALB/c) — parasite antigens in immune response

Perec

Okulewicz

2006

Helminthologia

View full text Add to dashboard Cite

203 SummaryIn conventional mice colonies, mouse pinworm, Syphacia obvelata is found very often. Several studies indicate that infection with this parasite can modulate the immune system of the host and can affect experimental final results. The aim of our study was to investigate the most immunogenic proteins of S. obvelata inducing both local and systemic immune response in naturally infected laboratory mice. Protein extracts of S. obvelata were analysed by Western blotting to examine their antigenic character. The antigens were probed with serum and mucosa of S. obvelata naturally infected mice. Surface and somatic antigens were recognized by serum and mucosal IgG, IgA and IgM antibodies. The most immunogenic and dominant proteins were observed. Proteins of Mw ~ 70, 65 and 48 kDa showed the most evident reaction with serum and mucosa antibodies of infected animals. Surface and somatic antigens of nematode S. obvelata eliciting immune response in laboratory mice may be useful in development of a diagnostic test which could be applied for the infection control prior the experiments.

show abstract

“…Cross & Klesius (1989) isolated a distinct fraction from O. ostertagi L3 extracts which can down-regulate humoral and cellular immune functions in mice. The surface polypeptide profiles of 3rd (L3) and 4thstage larvae (L4) have been examined (Keith et al 1990). The proteolytic enzymes present in, and secreted by, the different life-cycle stages of O. ostertagi were partially characterized (De Cock et al 1993).…”

Section: Take Down Policymentioning

confidence: 99%

Isolation, characterization and immunolocalization of a globin-like antigen from Ostertagia ostertagi adults

et al. 1996

View full text Add to dashboard Cite

Western blot analysis using an anti-globin rabbit serum Rb94 revealed a major band of 17 kDa in extracts of Ostertagia ostertagi adults and 4th-stage larvae. The adult stage globin-like antigen (OoAdGlb) was purified from total worm extracts by liquid chromatography. The protein has an estimated molecular mass of 36 kDa under non-reducing conditions, suggesting a dimeric structure containing 2 non-covalently linked 17 kDa monomers. Tryptic peptides were sequenced and showed strong similarities with the globins of free-living and parasitic nematodes. Immunolocalization revealed the presence of this globin-like antigen in the body wall musculature and/or the cuticle of O. ostertagi adults. An enzyme-linked immunosorbent assay based on the purified OoAdGlb showed no differences in response between calves infected by O. ostertagi and/or Cooperia oncophora and the negative controls.

show abstract

Stage-specific cuticular proteins of Ostertagia circumcincta and Ostertagia ostertagi

Cited by 15 publications

References 16 publications

Studies on the immunoglobulin E responses to Teladorsagia circumcincta in sheep: purification of a major high molecular weight allergen

Studies on the immunoglobulin E responses to Teladorsagia circumcincta in sheep: purification of a major high molecular weight allergen

The presence of Syphacia obvelata in laboratory mice (BALB/c) — parasite antigens in immune response

Isolation, characterization and immunolocalization of a globin-like antigen from Ostertagia ostertagi adults

Contact Info

Product

Resources

About