The presence of Syphacia obvelata in laboratory mice (BALB/c) — parasite antigens in immune response

Abstract

203 SummaryIn conventional mice colonies, mouse pinworm, Syphacia obvelata is found very often. Several studies indicate that infection with this parasite can modulate the immune system of the host and can affect experimental final results. The aim of our study was to investigate the most immunogenic proteins of S. obvelata inducing both local and systemic immune response in naturally infected laboratory mice. Protein extracts of S. obvelata were analysed by Western blotting to examine their antigenic character. The antigens were probed with serum and mucosa of S. obvelata naturally infected mice. Surface and somatic antigens were recognized by serum and mucosal IgG, IgA and IgM antibodies. The most immunogenic and dominant proteins were observed. Proteins of Mw ~ 70, 65 and 48 kDa showed the most evident reaction with serum and mucosa antibodies of infected animals. Surface and somatic antigens of nematode S. obvelata eliciting immune response in laboratory mice may be useful in development of a diagnostic test which could be applied for the infection control prior the experiments.

Potential anthelmintic effect of chitosan on Syphacia muris infecting Wistar rats: biochemical, immunological, and histopathological studies

“…Immunoglobulins IgE and IgG detection is essential for diagnosing numerous pathological conditions associated with bacterial, viral, and parasitic infections, thereby enabling the administration of the appropriate therapeutic treatment 57 . Our data revealed a significant rise ( P < 0.05) in total IgE and IgG levels in infected rats compared to the control group, which agrees with Michels et al 52 and Perec and Okulewicz 57 , who demonstrated an elevation in Syphacia -specific antibodies. Also, Wright and Bickle 58 stated an increase in hookworm-specific IgG and IgE levels in humans following infection.…”