Stage-specific events in tomato graft formation and the regulatory effects of auxin and cytokinin

Cui, Qingqing; Xie, Lulu; Dong, C.Y.; Gao, Lihong; Shang, Qingmao

doi:10.1016/j.plantsci.2020.110803

Cited by 32 publications

(30 citation statements)

References 57 publications

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“…In addition, NAC071 and NAC096 transmit auxin and ethylene signals, triggering xyloglucan endotransglucosylase/hydrolase cell wall organization, and promoting cambial cell formation after wounding (Pitaksaringkarn et al, 2014;Matsuoka et al, 2021). Although these patterns were observed in Arabidopsis, there are similar patterns in other species (Melnyk et al, 2018;Cui et al, 2021).…”

Section: Introductionmentioning

confidence: 88%

“…We then associated co-expression modules with certain traits. As reported in previous studies, the process of graft healing exhibited stage-specific features (Cui et al, 2021). According to the regular clustering pattern of samples based on the sample type and the time after grafting, four stages were set up as developmental traits: S1 for 6 and 12 HAG, S2 for 24 and 48 HAG, S3 for 72 and 120 HAG, and S4 for 168 and 240 HAG.…”

Section: Common Pathways In Arabidopsis and Tomato During Graft Healingmentioning

confidence: 95%

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Conserved Regulatory Pathways for Stock-Scion Healing Revealed by Comparative Analysis of Arabidopsis and Tomato Grafting Transcriptomes

et al. 2022

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Many plants can successfully join root and shoot sections at cut surfaces when severed at the stem. Graft healing is complex and conserved in diverse taxonomic groups with different vascular structures. Herein, we compared transcriptome data from autografted and separated stem sections of Arabidopsis thaliana and tomato (Solanum lycopersicum) to explore changes related to graft healing. Using orthologous gene pairs identified between the two species, temperal expression patterns of evolutionary associated genes in grafted top and bottom, separated top and bottom, and intact stems were exhibited. Genes with expression preference indicate functional diversification of genes related to anatomical structure and cellular development in the two species. Expression profiles of the variable genes revealed common pathways operating during graft healing, including phenylpropanoid metabolism, response to oxygen-containing compounds, xylan, and cell wall biogenesis, mitosis and the cell cycle, carboxylic acid catabolism, and meristem structural organization. In addition, vascular differentiation related NAC domain transcription factors and genome-wide members in Arabidopsis and tomato were used for phylogenetic and expression analysis. Expression differences were largely consistent with sequence differences, reflecting high similarity for protein-coding and regulatory regions of individual clades. NAC proteins mainly clustered in accordance with their reported functions in xylem differentiation or cambium formation. The putative conserved mechanisms suggested by conserved genes and functions could help to expand graft healing theory to a wider range of species, and temporal fluctuations in common pathways imply conserved biological processes during graft healing.

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Section: Introductionmentioning

confidence: 88%

Section: Common Pathways In Arabidopsis and Tomato During Graft Healingmentioning

confidence: 95%

Conserved Regulatory Pathways for Stock-Scion Healing Revealed by Comparative Analysis of Arabidopsis and Tomato Grafting Transcriptomes

et al. 2022

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“…Plant hormone signal transduction is associated with grafting 14 , 15 . The plant hormones play an important role in the reconnection of vascular bundles during the grafting process, as reviewed by Nanda and Melnyk 16 .…”

Section: Introductionmentioning

confidence: 99%

Light quality and quantity affect graft union formation of tomato plants

Ahmed

Ali

Rizwan

et al. 2021

Sci Rep

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It is already known that there are many factors responsible for the successful formation of a graft union. However, the role of light has been little studied. In an anatomical study, Scanning Electronic Microscope (SEM) was used to explore the effects of different light-emitting diodes (LEDs) on graft union formation in grafted tomato. In addition, the expression genes related to Auxin hormone signaling pathway (SAUR67, AUX1, ARF30, and LAX3) was investigated. The obtained results showed that the concrescence process occurred faster under R7:B3 light conditions, as compared to blue (B) and white fluorescent (WFL) lights. Red light application caused a delay in the vascular tissue differentiation, which may lead to callus development on both sides, causing junctional failure and resulting in ineffective graft junctional arrangement. The expression of genes related to Auxin hormone significantly increased by R7:B3 application. We suggest that LED spectra affects the graft development of tomato plants and can improve the performance of grafted tomato seedlings.

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“…The unpublished observations of our group clearly showed that the process of healing for the grafted watermelon seedlings is characterized by rapid vascular reconnection only 3 days after grafting which is significantly faster than tomato (c.f. Wang et al, 2019) in which the scion and rootstock reconnection was observed after 9 days (Cui et al, 2021). Upon its formation, callus rapidly differentiates in the vascular tissues (i.e., phloem and xylem), while plasmodesmata, the intracellular channels, form between the scion and rootstock cells.…”

Section: Discussionmentioning

confidence: 99%

Comparative Transcriptome Analysis in Homo- and Hetero-Grafted Cucurbit Seedlings

et al. 2021

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Watermelon (Citrullus lanatus) is a valuable horticultural crop with nutritional benefits grown worldwide. It is almost exclusively cultivated as grafted scions onto interspecific squash rootstock (Cucurbita maxima × Cucurbita moschata) to improve the growth and yield and to address the problems of soilborne diseases and abiotic stress factors. This study aimed to examine the effect of grafting (homo- and hetero-grafting) on the transcriptome level of the seedlings. Therefore, we compared homo-grafted watermelon (WW) with non-grafted watermelon control (W), homo-grafted squash (SS) with non-grafted squash control (S), hetero-grafted watermelon onto squash (WS) with SS, and WS with WW. Different numbers of differentially expressed genes (DEGs) were identified in each comparison. In total, 318 significant DEGs were detected between the transcriptomes of hetero-grafts and homo-grafts at 16 h after grafting. Overall, a significantly higher number of downregulated transcripts was detected among the DEGs. Only one gene showing increased expression related to the cytokinin synthesis was common in three out of four comparisons involving WS, SS, and S. The highest number of differentially expressed (DE) transcripts (433) was detected in the comparison between SS and S, followed by the 127 transcripts between WW and W. The study provides a description of the transcriptomic nature of homo- and hetero-grafted early responses, while the results provide a start point for the elucidation of the molecular mechanisms and candidate genes for the functional analyses of hetero-graft and homo-graft systems in Cucurbitaceae and generally in the plants.

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Stage-specific events in tomato graft formation and the regulatory effects of auxin and cytokinin

Cited by 32 publications

References 57 publications

Conserved Regulatory Pathways for Stock-Scion Healing Revealed by Comparative Analysis of Arabidopsis and Tomato Grafting Transcriptomes

Conserved Regulatory Pathways for Stock-Scion Healing Revealed by Comparative Analysis of Arabidopsis and Tomato Grafting Transcriptomes

Light quality and quantity affect graft union formation of tomato plants

Comparative Transcriptome Analysis in Homo- and Hetero-Grafted Cucurbit Seedlings

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