Stage‐specific expression of proprotein form of hyaluronan binding protein 1 (HABP1) during spermatogenesis in rat

Bharadwaj, Archana; Ghosh, Indraneel; Sengupta, Aniruddha; Cooper, Trevor G.; Weinbauer, Gerhard F.; Brinkworth, Martin H.; Nieschlag, Eberhard; Datta, Kasturi

doi:10.1002/mrd.10135

Cited by 18 publications

(8 citation statements)

References 31 publications

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“…p32 is synthesized as a pre protein and is processed by proteolytic cleavage of the N-terminal amino acids containing the mitochondrial signal sequence (27). The pre-protein form of p32 is only expressed in germ cells (28), which clearly justifies the predominant localization of p32 in the mitochondrial matrix.…”

Section: Introductionmentioning

confidence: 65%

p32/gC1qR is indispensable for fetal development and mitochondrial translation: importance of its RNA-binding ability

Yagi

Uchiumi

Takazaki

et al. 2012

Nucleic Acids Research

140

204

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p32 is an evolutionarily conserved and ubiquitously expressed multifunctional protein. Although p32 exists at diverse intra and extracellular sites, it is predominantly localized to the mitochondrial matrix near the nucleoid associated with mitochondrial transcription factor A. Nonetheless, its function in the matrix is poorly understood. Here, we determined p32 function via generation of p32-knockout mice. p32-deficient mice exhibited mid-gestation lethality associated with a severe developmental defect of the embryo. Primary embryonic fibroblasts isolated from p32-knockout embryos showed severe dysfunction of the mitochondrial respiratory chain, because of severely impaired mitochondrial protein synthesis. Recombinant p32 binds RNA, not DNA, and endogenous p32 interacts with all mitochondrial messenger RNA species in vivo. The RNA-binding ability of p32 is well correlated with the mitochondrial translation. Co-immunoprecipitation revealed the close association of p32 with the mitoribosome. We propose that p32 is required for functional mitoribosome formation to synthesize proteins within mitochondria.

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Section: Introductionmentioning

confidence: 65%

p32/gC1qR is indispensable for fetal development and mitochondrial translation: importance of its RNA-binding ability

Yagi

Uchiumi

Takazaki

et al. 2012

Nucleic Acids Research

140

204

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“…HABP1 has been demonstrated to play a role in spermatogenic differentiation. Stage-specific expression of HABP1 precursor during spermatogenesis in the rat (24). The sperm surface HABP1 level was correlated with the degree of sperm motility, an important criterion for fertilization (112).…”

Section: Hyaluronan Binding Proteinsmentioning

confidence: 99%

Hyaluronan as an Immune Regulator in Human Diseases

Jiang

Liang

Noble

2011

Physiological Reviews

900

914

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Accumulation and turnover of extracellular matrix components are the hallmarks of tissue injury. Fragmented hyaluronan stimulates the expression of inflammatory genes by a variety of immune cells at the injury site. Hyaluronan binds to a number of cell surface proteins on a variety of cell types. Hyaluronan fragments signal through both Toll-like receptor (TLR) 4 and TLR2 as well as CD44 to stimulate inflammatory genes in inflammatory cells. Hyaluronan is also present on the cell surface of epithelial cells and provides protection against tissue damage by interacting with TLR2 and TLR4 on these parenchymal cells. Hyaluronan and hyaluronan-binding proteins regulate inflammation, tissue injury and repair through regulating inflammatory cell recruitment, release of inflammatory cytokines, and stem cell migration. This review focuses on the role of hyaluronan as an immune regulator in human diseases.

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“…17 However no tissue expresses the proprotein form of HABP1 except the germ cells. 18 Another interesting feature of this protein is its diverse localization in different cell types under different physiological conditions in different cellular compartments including mitochondria 19,20 and nucleus 14,21 and is shown to be on the cell surface [22][23][24] and secreted into the extracellular matrix. 25,26 Though the true physiological roles of HABP1 remain to be clarified, it is speculated it may be involved in the cellular trafficking connecting different cellular compartments and the cell surface.…”

Section: Introductionmentioning

confidence: 99%

Upregulation of hyaluronan binding protein 1 (HABP1/p32/gC1qR) is associated with Cisplatin induced apoptosis

Kamal

Datta

2006

Apoptosis

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We have earlier reported that overexpression of HABP1 in fibroblast cells causes perturbed cell growth, extensive vacuolation and restricted entry to the S-phase, finally leading to apoptosis (Biochem Biophys Res Commun 2003; 300: 686-693). In the present study, we investigate the regulation of HABP1 expression in cisplatin induced apoptosis in HeLa cells. Apoptosis induced in HeLa cells at 24 h of cisplatin treatment was confirmed by nuclear fragmentation, increase in subdiploid population and the enhanced activation of ERK and upregulation of p53. In association with apoptosis induction, an upregulation of HABP1 expression was observed in HeLa cells at 18 and 24 h of cisplatin treatment. Quantification of HABP1 expression by flow cytometry confirmed a two-fold increase in total intracellular HABP1 expression at 24 h of cisplatin treatment. Under the same condition the HABP1 transcript level measured by semi quantitative RT PCR showed 2.5-fold increase ascertaining transcriptional regulation of HABP1 during cisplatin induced apoptosis. Further, in normal HeLa cells though a small amount of HABP1 can be detected in nucleus, but with apoptosis induction the protein is mainly concentrating around the nuclear periphery at 18 h of cisplatin treatment and is present both in the nucleus as well as in the cytosol at 24 h of treatment, suggesting its nuclear translocation during apoptosis. To substantiate our findings prior to the cisplatin treatment, the expression of HABP1 was reduced by small interfering RNA mediated knockdown. We observed a reduction in apoptotic cell population in cisplatin treated HeLa cells with disrupted HABP1 conferring resistance to cisplatin induced apoptosis. We report here that HABP1 upregulation in the cell is important for cisplatin induced apoptosis.

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Stage‐specific expression of proprotein form of hyaluronan binding protein 1 (HABP1) during spermatogenesis in rat

Cited by 18 publications

References 31 publications

p32/gC1qR is indispensable for fetal development and mitochondrial translation: importance of its RNA-binding ability

p32/gC1qR is indispensable for fetal development and mitochondrial translation: importance of its RNA-binding ability

Hyaluronan as an Immune Regulator in Human Diseases

Upregulation of hyaluronan binding protein 1 (HABP1/p32/gC1qR) is associated with Cisplatin induced apoptosis

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