Stage-Specific Localization of Basigin, a Member of the Immunoglobulin Superfamily, During Mouse Spermatogenesis.

Maekawa, Mamiko; Suzuki-Toyota, Fumie; Toyama, Yoshiro; Kadomatsu, Kenji; Hagihara, Mao; Kuno, Naohiko; Muramatsu, Takashi; Dohmae, Kayoko; Yuasa, Shigeki

doi:10.1679/aohc.61.405

Cited by 25 publications

(30 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In males, the intense basigin expression observed in Leydig cells and all stages of germ cells except spermatogonia is in agreement with previous reports (Igakura et al, 1998; Maekawa et al, 1998). Not surprisingly, Bsg–null mice exhibit a complete arrest of spermatogenesis at the metaphase of the first meiosis (Igakura et al, 1998).…”

Section: Discussionsupporting

confidence: 93%

Expression of basigin in reproductive tissues of estrogen receptor-α or -β null mice

Chen¹,

Bi²,

Nakai³

et al. 2010

Reproduction

View full text Add to dashboard Cite

Basigin plays important roles in both male and female reproduction because basigin (Bsg) null male and female mice are infertile. The aim of the present study was to determine whether basigin expression in reproductive organs requires oestrogen receptor (ER) α or ERβ. Expression of basigin protein in the testis, ovary and male and female reproductive tracts was studied in adult wild type, ERα-null (αERKO) and ERβ-null (βERKO) mice by immunohistochemistry and immunoblotting. Basigin mRNA levels in ovary and uterus were examined by quantitative RT-PCR. In females, basigin protein expression was observed mainly in granulosa and interstitial cells of the ovary and epithelial cells of the proximal oviduct in all genotypes. Basigin protein was also expressed in the uterine epithelium at prooestrus and oestrus in WT and βERKO mice but not in αERKO mice. However, a higher level of basigin mRNA was observed in uteri of αERKO mice compared with WT and βERKO mice. In males, basigin was expressed in Leydig cells and all germ cells except spermatogonia in all genotypes. Basigin was present in epithelial cells lining the efferent ductules in WT and βERKO mice but expression was greatly reduced in αERKO mice. In epididymal ducts, basigin expression was observed in epithelial cells in the caput and cauda in all genotypes. These data suggest that expression of basigin protein requires ERα, but not ERβ, in the uterus and efferent ductules, but is independent of ER in the ovary, oviduct, testis and epididymis.

show abstract

Section: Discussionsupporting

confidence: 93%

Expression of basigin in reproductive tissues of estrogen receptor-α or -β null mice

Chen¹,

Bi²,

Nakai³

et al. 2010

Reproduction

View full text Add to dashboard Cite

show abstract

“…In mouse testis, the expression of CD147 is reported to first appear at 7 days postpartum (dpp) and gradually increase in a developmental-dependent manner 50 . Several studies have demonstrated that CD147 is localized in mouse leptotene spermatocytes and step 9–11 spermatids 51 and on the head region of capacitated spermatozoa 52 . In our recent study, we further demonstrated that CD147 was also expressed in spermatogonia in both human and mouse testes 50 …”

Section: Role Of Cd147 In the Reproductive Systemmentioning

confidence: 74%

New insights into germ cell migration and survival/apoptosis in spermatogenesis

et al. 2012

View full text Add to dashboard Cite

CD147, also named basigin (Bsg) or extracellular matrix (ECM) metalloproteinase inducer (EMMPRIN), is a highly glycosylated protein first identified as a tumor cell surface molecule. In cancer, it is well established that CD147 promotes metastasis by stimulating the production of MMPs. Recent studies have also suggested that it may be associated with tumor growth and angiogenesis. Interestingly, CD147 is expressed in germ cells of different development stages in the testis and its knockout mice are infertile, indicating an essential role of CD147 in spermatogenesis. While the detailed involvement of CD147 in spermatogenesis remains elusive, our recent findings have revealed a dual role of CD147 in germ cell development. On the one hand, it regulates the migration of spermatogonia and spermatocytes via the induction of MMP-2 production; on the other hand, it specifically regulates the survival/apoptosis of spermatocytes but not spermatogonia through a p53-independent pathway. In this review, we aim to provide an overview on the functions of CD147, comparing its roles in cancer and the testis, thereby providing new insights into the regulatory mechanisms underlying the process of spermatogenesis.

show abstract

“…Thus, CD147 is considered as an essential tumor-specific marker in many cancers [15]. In adult mouse testis, CD147 is expressed on the cell surface of spermatogonia and spermatocytes, and gradually increases during the meiotic prophase [3, 16]. Knockout of CD147 leads to azoospermic phenotype, indicating that this gene is essential for spermatogenesis [17].…”

Section: Introductionmentioning

confidence: 99%

CD147 regulates extrinsic apoptosis in spermatocytes by modulating NFκB signaling pathways

et al. 2016

View full text Add to dashboard Cite

CD147 null mutant male mice are infertile with arrested spermatogenesis and increased apoptotic germ cells. Our previous studies have shown that CD147 prevents apoptosis in mouse spermatocytes but not spermatogonia. However, the underlying mechanism remains elusive. In the present study, we aim to determine the CD147-regulated apoptotic pathway in mouse spermatocytes. Our results showed that immunodepletion of CD147 triggered apoptosis through extrinsic apoptotic pathway in mouse testis and spermatocyte cell line (GC-2 cells), accompanied by activation of non-canonical NFκB signaling and suppression of canonical NFκB signaling. Furthermore, CD147 was found to interact with TRAF2, a factor known to regulate NFκB and extrinsic apoptotic signaling, and interfering CD147 led to the decrease of TRAF2. Consistently, depletion of CD147 by CRISPR/Cas9 technique in GC-2 cells down-regulated TRAF2 and resulted in cell death with suppressed canonical NFκB and activated non-canonical NFκB signaling. On the contrary, interfering of CD147 had no effect on NFκB signaling pathways as well as TRAF2 protein level in mouse spermatogonia cell line (GC-1 cells). Taken together, these results suggested that CD147 plays a key role in reducing extrinsic apoptosis in spermatocytes, but not spermatogonia, through modulating NFκB signaling pathway.

show abstract

Stage-Specific Localization of Basigin, a Member of the Immunoglobulin Superfamily, During Mouse Spermatogenesis.

Cited by 25 publications

References 34 publications

Expression of basigin in reproductive tissues of estrogen receptor-α or -β null mice

Expression of basigin in reproductive tissues of estrogen receptor-α or -β null mice

New insights into germ cell migration and survival/apoptosis in spermatogenesis

CD147 regulates extrinsic apoptosis in spermatocytes by modulating NFκB signaling pathways

Contact Info

Product

Resources

About