Stage-specific quercetin sulfation in the gut of <i>Mythimna separata</i> larvae (Lepidoptera: Noctuidae)

Aboshi, Takako; Ishida, Masahiro; Matsushita, Kazuhiro; Hirano, Yunosuke; Nishida, Ritsuo; Mori, Naoki

doi:10.1080/09168451.2014.877835

Cited by 4 publications

(2 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The synergistic effect of quercetin and nucleopolyhedrovirus was also found ( Shi et al, 2021 ). However, quercetin metabolism by microbes is still unclear ( Aboshi et al, 2014 ). In this study, quercetin degradation by R. terrigena was discovered, which provides new techniques for mass production of Thitarodes larvae by employing this bacterium onto P. viviparum roots and seeds, and for the control of insect pest by inhibiting this gut bacterium when the pests feed on the plants full of quercetin.…”

Section: Discussionmentioning

confidence: 99%

Plant quercetin degradation by gut bacterium Raoultella terrigena of ghost moth Thitarodes xiaojinensis

2022

View full text Add to dashboard Cite

Associated microbes of several herbivorous insects can improve insect fitness. However, the contribution of specific insect gut bacterium to plant toxin toxification for its host fitness remains scarce. Here, a gut bacterium Raoultella terrigena from the ghost moth Thitarodes xiaojinensis larvae was identified. This bacterium grew unhindered in the presence of Polygonum viviparum, which is a natural food for ghost moth larvae but showed significant growth inhibition and toxicity against Spodoptera litura. S. litura reared on artificial diets containing 5, 15 and 25% P. viviparum powder after 7 days coculture with R. terrigena were found to have shorter larval and pupal durations than on the diets containing P. viviparum powder but without R. terrigena coculture. HPLC analysis revealed that the content of quercetin in mineral medium containing 15% P. viviparum powder after 7 days coculture with R. terrigena was significantly decreased (79.48%) as compared with that in P. viviparum powder without R. terrigena coculture. In vitro fermentation further verified that R. terrigena could degrade 85.56% quercetin in Lucia-Bertani medium. S. litura reared on artificial diets containing 0.01, 0.05 and 0.1 mg/g quercetin after 48 h coculture with R. terrigena were also found to have shorter larval, prepupal and pupal durations, as well as higher average pupal weight and adult emergence rate than on the diets containing quercetin, but without R. terrigena coculture. In addition, R. terrigena was detected in the bud and root tissues of the sterilized P. viviparum, indicating that T. xiaojinensis larvae might acquire this bacterium through feeding. These results demonstrate that the gut bacteria contribute to the degradation of plant toxic molecules to improve the development of herbivorous insects and provide fundamental knowledge for developing effective methods for beneficial insect rearing and pest control.

show abstract

Section: Discussionmentioning

confidence: 99%

Plant quercetin degradation by gut bacterium Raoultella terrigena of ghost moth Thitarodes xiaojinensis

2022

View full text Add to dashboard Cite

show abstract

“…A recent study on the metabolism of quercetin in oriental armyworm Mythimna separata larvae identified two metabolites (quercetin-4′-O- sulphate and quercetin 3-O-gludoside). The authors stated that sulfation could be important in detoxification and/or excretion of quercetin (Aboshi et al , 2014). In addition, 5-O-glucoside of quercetin was identified in the midgut tissues of silkworm Bombyx mori larvae after oral administration of quercetin, while quercetin 5,4′-di-O-glucoside was the major constituent in the haemolymph and silk glands (Hirayama et al , 2008).…”

Section: Discussionmentioning

confidence: 99%

Effects of dietary quercetin on performance and cytochrome P450 expression of the cotton bollworm,Helicoverpa armigera

Liu

Yuan

et al. 2015

Bull. Entomol. Res.

View full text Add to dashboard Cite

Quercetin is ubiquitous in terrestrial plants. The cotton bollworm Helicoverpa armigera as a highly polyphagous insect has caused severe crop losses. Until now, interactions between this pest and quercetin are poorly understood at the biochemical and molecular levels. In this study, we investigated the in vivo effects of quercetin on performance of cotton bollworm and on cytochrome P450 (P450) expression. Deleterious effects of quercetin on the performance of the cotton bollworm, including growth, survival, pupation and adult emergence were observed after oral administration of 3 and 10 mg g(-1) quercetin to larvae since the third instar, whereas no significant toxic effect was found at 0.1 mg g(-1) quercetin treatment. Piperonyl butoxide treatment enhanced the toxicity of quercetin. In vitro metabolism studies showed that quercetin was rapidly transformed by gut enzymes of fifth instar larvae of the cotton bollworm. qRT-PCR results revealed that the effect of quercetin on P450 expression was tissue- and dose-specific. Quercetin regulated P450 expression in a mild manner, and it could serve as P450 inducer (CYP337B1, CYP6B6) or repressor (CYP337B1, CYP6B7, CYP6B27, CYP9A14, CYP6AE11, and CYP4M7). These findings are important for advancing our understanding of the biochemical and molecular response of insects to plant toxins and have implications for a smart pest control.

show abstract

Molecular docking analyses of CYP450 monooxygenases of Tribolium castaneum (Herbst) reveal synergism of quercetin with paraoxon and tetraethyl pyrophosphate: in vivo and in silico studies

Ghaffar

Sehgal

Fatima

et al. 2020

Toxicology Research

View full text Add to dashboard Cite

Abstract Pest management in stored grain industry is a global issue due to the development of insecticide resistance in stored grain insect pests. Excessive use of insecticides at higher doses poses a serious threat of food contamination and residual toxicity for grain consumers. Since the development of new pesticide incurs heavy costs, identifying an effective synergist can provide a ready and economical tool for controlling resistant pest populations. Therefore, the synergistic property of quercetin with paraoxon and tetraethyl pyrophosphate has been evaluated against the larvae and adults of Tribolium castaneum (Herbst). Comparative molecular docking analyses were carried out to further identify the possible mechanism of synergism. It was observed that quercetin has no insecticidal when applied at the rate of 1.5 and 3.0 mg/g; however, a considerable synergism was observed when applied in combination with paraoxon. The comparative molecular docking analyses of CYP450 monooxygenase (CYP15A1, CYP6BR1, CYP6BK2, CYP6BK3) family were performed with quercetin, paraoxon and tetraethyl pyrophosphate which revealed considerable molecular interactions, predicting the inhibition of CYP450 isoenzyme by all three ligands. The study concludes that quercetin may be an effective synergist for organophosphate pesticides depending upon the dose and type of the compound. In addition, in silico analyses of the structurally diversified organophosphates can effectively differentiate the organophosphates which are synergistic with quercetin.

show abstract

Stage-specific quercetin sulfation in the gut of Mythimna separata larvae (Lepidoptera: Noctuidae)

Cited by 4 publications

References 7 publications

Plant quercetin degradation by gut bacterium Raoultella terrigena of ghost moth Thitarodes xiaojinensis

Plant quercetin degradation by gut bacterium Raoultella terrigena of ghost moth Thitarodes xiaojinensis

Effects of dietary quercetin on performance and cytochrome P450 expression of the cotton bollworm,Helicoverpa armigera

Molecular docking analyses of CYP450 monooxygenases of Tribolium castaneum (Herbst) reveal synergism of quercetin with paraoxon and tetraethyl pyrophosphate: in vivo and in silico studies

Contact Info

Product

Resources

About