Stage‐specific regulation of purine metabolism during infectious growth and sexual reproduction in Fusarium graminearum

Sun, Manli; Bian, Zhuyun; Luan, Qiaoqiao; Chen, Yitong; Wang, Wei; Dong, Yongrong; Chen, Lingfeng; Hao, Chaofeng; Xu, Jin‐Rong; Liu, Huiquan

doi:10.1111/nph.17170

Cited by 20 publications

(23 citation statements)

References 75 publications

(112 reference statements)

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“…Matching metabolites related to purine ring from "Caffeine biosynthesis" highlight triggering of nitrogen reservoir during FHB in wheat as was previously observed [99]. Adenosine, guanosine and their phosphorylated forms of IMP, AMP and GMP are critical metabolites for vegetative growth, sexual/asexual reproduction and pathogenesis in F. graminearum [100]. In addition, adenine phosphoribosyltransferase has been reported as critical in enhancing oxidative stress tolerance in A. thaliana [101].…”

Section: Genotype-specific Metabolomic Response To Fhbmentioning

confidence: 52%

Metabolomic Aspects of Conservative and Resistance-related Elements of Response to Fusarium culmorum in the Grass Family

Piasecka¹,

Sawikowska²,

Witaszak³

et al. 2022

Preprint

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Background: Fusarium head blight (FHB) is a serious fungal disease of crop plants due to substantial yield reduction and production of mycotoxins in the infected grains. The breeding progress in increasing resistance with maintaining a high yield is not possible without a thorough examination of the molecular basis of plant immunity responses; Methods: LC-MS based metabolomics approaches powered by three-way ANOVA and differentially accumulated metabolites (DAMs) selection, correlation network and functional enrichment were conducted on grains of resistant and susceptible to FHB genotypes of barley and wheat as well as model grass Brachypodium distachyon (Bd) still poorly known at metabolomic level; Results: We selected common and genotype-specific DAMs in response to F. culmorum inoculation. Immunological reaction at metabolomic level was strongly diversified between resistant and susceptible genotypes. DAMs common for all tested species from porphyrins, flavonoids and phenylpropanoids metabolic pathways were highly correlated and reflects conservativeness in FHB response in Poaceae family. Resistant related DAMs belonged to different structural classes including tryptophan derived metabolites, pirimidines, amino acids proline and serine as well as phenylpropanoids and flavonoids. Physiological response to F. culmorum of Bd was close to barley and wheat genotypes however, metabolomic changes were strongly diversified. Conclusions: Combined targeted and untargeted metabolomics provides comprehensive knowledge about significant elements of plant immunity with potential of being molecular biomarkers of enhance resistance to FHB in grass family. Thorough examination of Bd21 metabolome in juxtaposition with barley and wheat diversified genotypes facilitate their setting as model grass for plant-microbe interaction.

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Section: Genotype-specific Metabolomic Response To Fhbmentioning

confidence: 52%

Metabolomic Aspects of Conservative and Resistance-related Elements of Response to Fusarium culmorum in the Grass Family

Piasecka¹,

Sawikowska²,

Witaszak³

et al. 2022

Preprint

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“…In the presence of combined stress, a single pathway related to purine nucleotide production and degradation was enriched. It has been shown that biosynthesis and degradation of purine nucleotides play an essential role in the growth and virulence of various fungal communities; microbial communities might be using these pathways for better growth and survival under combined stress (105,106). Microbial function in the ecosystem is determined not just because of the number and composition of taxa but because of the various positive, negative, direct, or indirect associations among the community members (107).…”

Section: Given the Impact Of Altered [O3] Resulting In A Possible Com...mentioning

confidence: 99%

Biotic and abiotic stress distinctly drive the phyllosphere microbial community structure

Bhandari

Sanz‐Sáez²,

Leisner

et al. 2022

Preprint

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While the physiological and transcriptional response of the host to biotic and abiotic stresses have been intensely studied, little is known about the resilience of associated microbiomes and their contribution towards tolerance to these stresses. We evaluated the impact of one such abiotic stress, elevated tropospheric ozone (O3), under open-top chamber field conditions on host susceptibility and phyllosphere microbiome associated with pepper cultivars resistant and susceptible to Xanthomonas. Pathogen challenge resulted in distinct microbial community structures in both cultivars under an ambient environment. Elevated O3alone affected microbial community structure associated with resistant cultivar but not the susceptible cultivar, indicating the role of host genotypic background in response to abiotic stress. Elevated O3did not influence overall host susceptibility but did increase disease severity on the resistant cultivar, indicating a possible compromise in the resistance. Interestingly, combined stress resulted in a shift in microbial composition and structure like that observed with pathogen challenge alone. It indicates the possible prioritization of community response towards the most significant stress and pathogen being most influential regardless of the cultivar. Despite community composition differences, overall functional redundancy was observed in the phyllosphere community. To gain insights into community-level interactions, network topology assessment indicated a stable network with enhanced taxon connectedness upon pathogen challenge. However, an observation of destabilized random network with a shift in hub taxa in the presence of combined stress warrants future studies on the consequences of such unstable microbial communities on host response to pathogens in the face of climate change.

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“…Matching metabolites related to purine rings from ”Caffeine biosynthesis” highlighted the triggering of nitrogen reservoirs during FHB in wheat, as was previously observed [ 76 ]. Adenosine, guanosine, and their phosphorylated forms of IMP, AMP, and GMP are critical metabolites for vegetative growth, sexual/asexual reproduction, and pathogenesis in F. graminearum [ 77 ]. Some transcripts encoding enzymes from purine metabolism were reported to be FHB-resistance-related in wheat [ 78 ].…”

Section: Discussionmentioning

confidence: 99%

Metabolomic Aspects of Conservative and Resistance-Related Elements of Response to Fusarium culmorum in the Grass Family

Piasecka

Sawikowska

Witaszak

et al. 2022

Cells

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Background: Fusarium head blight (FHB) is a serious fungal disease affecting crop plants, causing substantial yield reductions and the production of mycotoxins in the infected grains. Achieving progress in the breeding of crops with increased resistance and maintaining a high yield is not possible without a thorough examination of the molecular basis of plant immunity responses. Methods: LC-MS-based metabolomics approaches powered by three-way ANOVA and the selection of differentially accumulated metabolites (DAMs) were used for studying plant immunity. A correlation network and functional enrichment analysis were conducted on grains of barley and wheat genotypes that were resistant or susceptible to FHB, as well as on the model grass Brachypodium distachyon (Bd), as this is still poorly understood at the metabolomic level. Results: We selected common and genotype-specific DAMs in response to F. culmorum inoculation. The immunological reaction at the metabolomic level was strongly diversified between resistant and susceptible genotypes. DAMs that were common to all tested species from the porphyrin, flavonoid, and phenylpropanoid metabolic pathways were highly correlated, reflecting conservativeness in the FHB response in the Poaceae family. Resistance-related DAMs belonged to different structural classes, including tryptophan-derived metabolites, pyrimidines, the amino acids proline and serine, as well as phenylpropanoids and flavonoids. The physiological response to F. culmorum of Bd was close to that of barley and wheat genotypes; however, metabolomic changes were strongly diversified. Conclusions: Combined targeted and untargeted metabolomics provides comprehensive knowledge about significant elements of plant immunity that have the potential to be molecular biomarkers of enhanced resistance to FHB in the grass family. Thorough examination of the Bd metabolome in juxtaposition with diversified genotypes of barley and wheat facilitated its use as a model grass for plant–microbe interaction.

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Stage‐specific regulation of purine metabolism during infectious growth and sexual reproduction in Fusarium graminearum

Cited by 20 publications

References 75 publications

Metabolomic Aspects of Conservative and Resistance-related Elements of Response to <em>Fusarium culmorum</em> in the Grass Family

Metabolomic Aspects of Conservative and Resistance-related Elements of Response to <em>Fusarium culmorum</em> in the Grass Family

Biotic and abiotic stress distinctly drive the phyllosphere microbial community structure

Metabolomic Aspects of Conservative and Resistance-Related Elements of Response to Fusarium culmorum in the Grass Family

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