Staining and Derivatization Techniques for Visualization in Planar Chromatography

Zarzycki, Paweł K.

doi:10.1016/b978-0-12-417223-4.00008-x

Cited by 8 publications

(8 citation statements)

References 29 publications

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“…Planar chromatography is one of the first analytical methods where derivatization procedures were applied mainly for spot visualization (Zarzycki, 2015). Derivatization is also widely used in MALDI-MS and MALDI imaging for enhancing desorption/ ionization efficiencies of the analytes (Zaikin and Halket, 2009;Zaikin and Borisov, 2021).…”

Section: Derivatizationmentioning

confidence: 99%

Recent Advances in Combinations of TLC With MALDI and Other Desorption/Ionization Mass-Spectrometry Techniques

2021

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The combination of planar chromatography with desorption/ionization mass-spectrometry (MS) techniques provides chemists with unique tools for fast and simple separation of mixtures followed by the detection of analytes by the most powerful analytical method. Since its introduction in the early 1990s, thin-layer chromatography (TLC)/matrix-assisted mass spectrometry (MALDI) has been used for the analysis of a wide range of analytes, including natural and synthetic organic compounds. Nowadays, new desorption/ionization approaches have been developed and applied in conjunction with planar chromatography competing with MALDI. This review covers recent developments in the combination of TLC with various desorption/ionization MS methods which were made in recent several years.

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Section: Derivatizationmentioning

confidence: 99%

Recent Advances in Combinations of TLC With MALDI and Other Desorption/Ionization Mass-Spectrometry Techniques

2021

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“…Spraying, dipping, or contact with a transfer pad (overpressure derivatization) is used for reagents in solution. Spray techniques using simple atomizers have a long history in TLC [48,49]. Reagent application by this method, however, is quite difficult to perform well.…”

Section: Postchromatographic Reactionsmentioning

confidence: 99%

Instrument platforms for thin-layer chromatography

Bernard-Savary¹,

Poole

2015

Journal of Chromatography A

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“…The following solvent mixture was used as a mobile phase 1, ethyl acetate: methanol: water (100:13.5:10 v/v/v), and mobile phase 2, butanol: acetic acid: water (25:1:24, v/v/v). The plates were revealed with different staining reagents, following previously described protocols by [21,22].…”

Section: Thin Layer Chromatography (Tlc)mentioning

confidence: 99%

Phytochemical Screening of Callus and Cell Suspensions Cultures of Thevetia peruviana

Meza

Arias

Cuaspud

et al. 2020

Braz. arch. biol. technol.

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Thevetia peruviana is an ornamental shrub grown-up in many tropical region of the world. This plant produces secondary metabolites with biological properties of interest for the pharmaceutical industry. The objective was to determine the secondary metabolites profile of callus and cell suspension cultures of T. peruviana and compare them with those from explant (fruit pulp). Extracts in 50% aqueous ethanol and ethyl acetate were prepared. The phytochemical analysis was performed using standard chemical tests and thin layer chromatography. In addition, total phenolic and flavonoids compounds (TPC and TFC), total cardiac glycosides (TCG) and total antioxidant activity (TAA) was determined during the cell suspension growth. Phenolic chemical profile was also analyzed by high performance liquid chromatography (HPLC). Common metabolites (alkaloids, amino acids, antioxidants, cardiac glycosides, leucoanthocyanidins, flavonoids, phenols, sugars and triterpenes) were detected in all samples. The maximum production of extracellular TCG, TPC, TFC and TAA in cells suspensions were at 6-12 days; in contrast, intracellular content was relatively constant during the exponential grown phase (0 to 12-days). HPLC analysis detected one compound with retention time at 11.6 min; this compound was tentatively identified as dihydroquercetin, a flavonoid with anti-cancer properties. These results provide evidence on the utility of the in vitro cell cultures of T. peruviana for valuable pharmaceutical compounds production. HIGHLIGHTS Callus and cell suspensions of T. peruviana have similar phytochemical profile to in vivo plant. Cell culture of T. peruviana is a reliable platform for high-value metabolites production. Cardiac glycosides and phenolic are the most valuable metabolites detected in plant cell cultures  Dihydroquercetin production in a free and conjugated form in cell cultures is highlighted 2 Mendoza, D.; et al.

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Staining and Derivatization Techniques for Visualization in Planar Chromatography

Cited by 8 publications

References 29 publications

Recent Advances in Combinations of TLC With MALDI and Other Desorption/Ionization Mass-Spectrometry Techniques

Recent Advances in Combinations of TLC With MALDI and Other Desorption/Ionization Mass-Spectrometry Techniques

Instrument platforms for thin-layer chromatography

Phytochemical Screening of Callus and Cell Suspensions Cultures of Thevetia peruviana

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