Staining of Reticulocytes by Brilliant Cresyl Blue

Heath, Clark W.; Daland, Geneva A.

doi:10.1001/archinte.1931.00150010138009

Cited by 17 publications

(2 citation statements)

References 19 publications

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“…Hematological estimations were carried out using a MEK-6318 K Automated Hematology Analyzer (Nihon-Kodhen Co., Tokyo, Japan) for erythrocyte counts (RBC), count hemoglobin concentration (HB), hematocrit value (HCT), mean corpuscular volume (MCV), mean corpuscular hemoglobin (MCH), mean corpuscular hemoglobin concentration (MCHC), red blood cell distribution width (RDW-CV), platelet counts (PLT), mean platelet volume (MPV), platelet distribution width (PDW), plateletcrit (PCT), and leukocyte counts (WBC). Differential cell count (DC) (including lymphocyte (Lympho), monocyte (Mono), granulocyte (Gran)) and reticulocyte (Retic) were carried out with blood smear samples that were stained with Wright-Giemsa and brilliant cresyl blue, 22 respectively.…”

Section: Hematology and Blood Coagulationmentioning

confidence: 99%

Intravenous repeated-dose toxicity study of ZnPcS2P2-based-photodynamic therapy in Wistar rats

Zhang

Jin

Bao

et al. 2006

Photochem Photobiol Sci

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The purpose of the current study was to investigate the potential repeated-dose toxicity of ZnPcP2S2-based photodynamic therapy (ZnPc-PDT) in Wistar rats. The animals were administered ZnPcS2P2 intravenously ten times successively every 4 d and irradiated with a 670 nm laser light for 6 min at subsequent 48 h and 72 h. At the end of the treatment period, 10 rats/sex/group were sacrificed, while 5 rats/sex/group were sacrificed after a two-week recovery period. During the test period, clinical signs, mortality, body weights, food and water consumption, ophthalmoscopy, hematology, serum biochemistry, urinalysis, organ weights, gross findings and histopathology were examined. The association between the increased liver weight and hepatic spotty and lytic necrosis seen in high dose females corroborates the conclusion that high dose ZnPc-PDT could induce hepatic injury in Wistar rats and they are probably related to the abnormality of certain biochemical parameters of females in the high dose group. Furthermore, microscopic examination for the ZnPc-PDT groups shows the presence of some Kelly and khaki granules in Kupffer cells and endothelia of the livers, epithelia of the renal tubules, marginal sinus and medulla of the spleens, alveolar walls of the lungs, reticular cells and macrophages of the mesenteric lymph nodes, testicular Leydig cells, epididymal epithelial cells, endometrial stromal cells, and interstitial cells and corpora lutea of the ovaries from all or most of the animals. There were no adverse effects on mortality, clinical signs, food and water consumption, ophthalmoscopy, uranalysis, hematology, serum biochemistry, body weights and necropsy findings in control, low and mid dose groups. Based on these results, it was concluded that the intravenous repeated-dose of ZnPcP2S2-PDT induced the abnormalities of liver weights, hepatic biochemistry and histopathology, and pigmentation in the several important organs in Wistar rats at 4 mg kg(-1) d(-1). The target organ was determined to be liver (and spleen perhaps), but this was not so obvious in males. The no-observed-adverse-effect level (NOAEL) was considered to be 1.0 mg kg(-1) for both sexes.

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Section: Hematology and Blood Coagulationmentioning

confidence: 99%

Intravenous repeated-dose toxicity study of ZnPcS2P2-based-photodynamic therapy in Wistar rats

Zhang

Jin

Bao

et al. 2006

Photochem Photobiol Sci

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“…Intra-vital staining according to Heath and Daland [10] was used. The reticulocytes were counted in each 1000 of erythrocytes encountered.…”

Section: Cytological Determinations Reticulocyte Determinationmentioning

confidence: 99%

Changes in Morphological and Cytological Properties of Blood During Physical Exercise in Water at 4°C and 25°C

Teległów¹,

Bilski²,

Dąbrowski³

et al. 2011

Medicina Sportiva

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Objective: The aim of this study was to determine the effect of a single swimming exercise till exhaustion, in water on the morphological and cytological blood parameters in laboratory rats.Methods: The experiment involved 60 male rats from the Wistar strain, aged 8 weeks and 36 weeks. The animals were separated into 6 groups: 3 groups (I, II, III) of 8-week old animals, and 3 groups (IV, V, VI) of 36-week old animals. The rats in the study groups i.e. II, III, V, and VI were made to swim till exhaustion in water at 4ºC and at 25°C. After the experiment blood samples were collected from each rat and hematological and cytological determinations were completed for each sample.Results: Compared with the control group, in the rats from group II (8-week old, swimming in water at 4ºC) a significant increase was noted in the RBC count, Hb, Ht, and MCHC, and MCH indices, whereas in the rats from group V (36-week old, swimming in water at 4ºC) increases were noted in the MCHC and MCH indices (again when compared with the control group). In the rats from group III (8-week old, swimming in water at 25ºC), compared with the control group, significant increases were noted in the Hb and Ht parameters, as well as in the MCHC, and MCH indices, whereas in the group VI rats (36-week old swimming at 25ºC) -the increase was noted in the MCHC index. No alterations were found in the reticulocyte count, and in poikilocytes during exercise in water at low temperatures in either the 8-week or 36-week old rats.Conclusions: The increases in the majority of blood parameters, testifies to the dehydration of the body during physical exercise which in turn affects the microcirculation function. The lack of alterations in the reticulocyte count in the peripheral blood may, in turn, testify to the ineffectiveness of low temperature during exercise upon the function of the bone marrow.

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A Method for the Simultaneous Enumeration of Blood Platelets and Reticulocytes

Dameshek¹

1932

Arch Intern Med (Chic)

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Staining of Reticulocytes by Brilliant Cresyl Blue

Cited by 17 publications

References 19 publications

Intravenous repeated-dose toxicity study of ZnPcS2P2-based-photodynamic therapy in Wistar rats

Intravenous repeated-dose toxicity study of ZnPcS2P2-based-photodynamic therapy in Wistar rats

Changes in Morphological and Cytological Properties of Blood During Physical Exercise in Water at 4°C and 25°C

A Method for the Simultaneous Enumeration of Blood Platelets and Reticulocytes

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