Eighteen male Wistar rats were divided into 3 groups of 6 animals each. Two groups received different intraperitoneal doses of TCDD (0.75 and 8Fg) in DMSO solution and the third group (control) received only DMSO on days 0, 7 and 14. On day 21 the animals were sacrificed, and then blood tests, pathological examination and CYP1A1 activity measurement were performed. In rats that received a high dose of dioxin (8 Fg) hepatic lobules revealed parenchymal degeneration and vacuolization of hepatocytes was observed, and also an increased CYP reaction was found in central parts of lobules, around the central vein. The reaction in control and low dose groups was weak. The resorufin level was significantly (P<0.05) higher in the group receiving a low dose of dioxin as compared to the control group. The study confirmed that TCDD damages the rat liver in a dose-dependent manner. Administration of high TCDD doses causing major liver damage also damaged CYP1A1 (based on higher resorufin levels in epiluminescence). TCDD activates CYP1A1, which was confirmed by increased immunohistochemical reactivity of central areas of hepatic lobules.
Objective: The aim of this study was to determine the effect of a single swimming exercise till exhaustion, in water on the morphological and cytological blood parameters in laboratory rats.Methods: The experiment involved 60 male rats from the Wistar strain, aged 8 weeks and 36 weeks. The animals were separated into 6 groups: 3 groups (I, II, III) of 8-week old animals, and 3 groups (IV, V, VI) of 36-week old animals. The rats in the study groups i.e. II, III, V, and VI were made to swim till exhaustion in water at 4ºC and at 25°C. After the experiment blood samples were collected from each rat and hematological and cytological determinations were completed for each sample.Results: Compared with the control group, in the rats from group II (8-week old, swimming in water at 4ºC) a significant increase was noted in the RBC count, Hb, Ht, and MCHC, and MCH indices, whereas in the rats from group V (36-week old, swimming in water at 4ºC) increases were noted in the MCHC and MCH indices (again when compared with the control group). In the rats from group III (8-week old, swimming in water at 25ºC), compared with the control group, significant increases were noted in the Hb and Ht parameters, as well as in the MCHC, and MCH indices, whereas in the group VI rats (36-week old swimming at 25ºC) -the increase was noted in the MCHC index. No alterations were found in the reticulocyte count, and in poikilocytes during exercise in water at low temperatures in either the 8-week or 36-week old rats.Conclusions: The increases in the majority of blood parameters, testifies to the dehydration of the body during physical exercise which in turn affects the microcirculation function. The lack of alterations in the reticulocyte count in the peripheral blood may, in turn, testify to the ineffectiveness of low temperature during exercise upon the function of the bone marrow.
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