Standardisation of sheep model for endodontic regeneration/revitalisation research

Altaii, Milad; Broberg, Marita; Cathro, Peter; Richards, Lindsay

doi:10.1016/j.archoralbio.2016.01.008

Cited by 16 publications

(19 citation statements)

References 34 publications

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“…The similarity of sheep teeth to human teeth (Altaii et al . ) allows the use of normal endodontic instruments and helps to validate the use of sheep as an appropriate animal model.…”

Section: Discussionmentioning

confidence: 99%

“…In this in vivo study, the endodontic regeneration/revitalization protocol in immature infected sheep teeth generally showed positive outcomes with narrowing of the apices, thickening of the walls and increase in root length. The similarity of sheep teeth to human teeth (Altaii et al 2016) allows the use of normal endodontic instruments and helps to validate the use of sheep as an appropriate animal model. Histology sections showed three different revitalization regions 6 months after treatment.…”

Section: Discussionmentioning

confidence: 99%

“…Altaii et al . (), showed that the anatomy of sheep teeth have many similarities to human teeth, with each dental age having advantages and disadvantages in their use for investigating regeneration/revitalization. This study aims to assess the effectiveness of an endodontic regeneration/revitalization protocol using immature infected sheep teeth and to compare the outcomes with those reported in the literature.…”

Section: Introductionmentioning

confidence: 99%

“…Sheep have been used in many dental studies in other areas of research (Danesh-Meyer et al 1997, Schliephake et al 2001, Langhoff et al 2008), but no previous in vivo studies have investigated their suitability for endodontic regeneration/revitalization research. Altaii et al (2016), showed that the anatomy of sheep teeth have many similarities to human teeth, with each dental age having advantages and disadvantages in their use for investigating regeneration/revitalization. This study aims to assess the effectiveness of an endodontic regeneration/revitalization protocol using immature infected sheep teeth and to compare the outcomes with those reported in the literature.…”

Section: Introductionmentioning

confidence: 99%

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Endodontic regeneration and tooth revitalization in immature infected sheep teeth

et al. 2016

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“…The similarity of sheep teeth to human teeth (Altaii et al . ) allows the use of normal endodontic instruments and helps to validate the use of sheep as an appropriate animal model.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Endodontic regeneration and tooth revitalization in immature infected sheep teeth

et al. 2016

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“…Because of these difficulties, in vivo studies on pulp-dentin complex regeneration to date have usually involved ectopic transplantation of the candidate substance into the subcutaneous tissue or renal capsule rather than orthotopic transplantation directly into the teeth [5]. Only several studies have been performed the orthotopic transplantation of a candidate substance in large animals such as dogs, pigs, ferrets, and monkeys [678]. However, before applying these candidate substances in clinical trials, their treatment efficacies and safeties should be evaluated using in vivo orthotopic transplantation in a sufficient number of animals.…”

Section: Introductionmentioning

confidence: 99%

Development of a mouse model for pulp-dentin complex regeneration research: a preliminary study

et al. 2019

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Objectives To achieve pulp-dentin complex regeneration with tissue engineering, treatment efficacies and safeties should be evaluated using in vivo orthotopic transplantation in a sufficient number of animals. Mice have been a species of choice in which to study stem cell biology in mammals. However, most pulp-dentin complex regeneration studies have used large animals because the mouse tooth is too small. The purpose of this study was to demonstrate the utility of the mouse tooth as a transplantation model for pulp-dentin complex regeneration research. Materials and Methods Experiments were performed using 7-week-old male Institute of Cancer Research (ICR) mice; a total of 35 mice had their pulp exposed, and 5 mice each were sacrificed at 1, 2, 4, 7, 9, 12 and 14 days after pulp exposure. After decalcification in 5% ethylenediaminetetraacetic acid, the samples were embedded and cut with a microtome and then stained with hematoxylin and eosin. Slides were observed under a high-magnification light microscope. Results Until 1 week postoperatively, the tissue below the pulp chamber orifice appeared normal. The remaining coronal portion of the pulp tissue was inflammatory and necrotic. After 1 week postoperatively, inflammation and necrosis were apparent in the root canals inferior to the orifices. The specimens obtained after experimental day 14 showed necrosis of all tissue in the root canals. Conclusions This study could provide opportunities for researchers performing in vivo orthotopic transplantation experiments with mice.

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Animal Models in Dental Research

Nokhbatolfoghahaei

Paknejad

Bohlouli

et al. 2019

Applications of Biomedical Engineering in Dentistry

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Standardisation of sheep model for endodontic regeneration/revitalisation research

Cited by 16 publications

References 34 publications

Endodontic regeneration and tooth revitalization in immature infected sheep teeth

Endodontic regeneration and tooth revitalization in immature infected sheep teeth

Development of a mouse model for pulp-dentin complex regeneration research: a preliminary study

Animal Models in Dental Research

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