Standardisation of the haemagglutination test for peste des petits ruminants (PPR)

Ezeibe, Maduike C. O.; Wosu, L.O.; Erumaka, I.

doi:10.1016/s0921-4488(03)00123-8

Cited by 17 publications

(26 citation statements)

References 4 publications

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“…Some of these test (ELISA, RT-PCR and isolation) are expensive, require skilled personals and sophisticated laboratory. However, HA is a simple and cheap test and has been used for the diagnosis of PPR (Wosu, 1985;Ezeibe et al, 2004). For example, a study reported HA activity of porcine RBCs with extract from PPR infected mesenteric lymph nodes (Wosu, 1985).…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of Haemagglutination Assay (HA) for the Detection of Peste des Petits Ruminants Virus (PPRV) in Faecal Samples of Recovered Goats

Latif¹

2014

Res. j. vet. pract.

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Section: Discussionmentioning

confidence: 99%

“…The HA test was performed as described by Wosu (1985) and Ezeibe et al,(2004). Briefly, two fold serial dilutions of each faecal sample suspension were made in 50 µL of sterile PBS (pH 7.2) in U bottom microtitration plate.…”

Section: Haemagglutination Testmentioning

confidence: 99%

Evaluation of Haemagglutination Assay (HA) for the Detection of Peste des Petits Ruminants Virus (PPRV) in Faecal Samples of Recovered Goats

Latif¹

2014

Res. j. vet. pract.

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“…Thus the haemagglutination test was able to differentiate PPRV from RPV and to confirm PPRV infection. Wosu (1985) found that only piglet RBCs consistently gave positive results with the homogenate antigen although Ramachandran et al (1993) and Ezeibe et al (2004) demonstrated the agglutination of RBCs of different animal species by PPRV.…”

Section: Discussionmentioning

confidence: 99%

“…PPR virus like measles virus has haemagglutination properties (Wosu 1985;Wosu 1991;Ramachandran et al 1993;Ezeibe et al 2004). Haemagglutination test unlike agar gel precipitation test was able to differentiate PPRV from RPV which has no haemagglutinating properties and unable to agglutinate RBCs (Huygelen 1960).…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of Peste des Petits Ruminants (PPR) virus antigen in Sudan by agar gel precipitation (AGPT) and haemagglutination (HA) Tests

Osman

Rahman²,

Ali

et al. 2007

Trop Anim Health Prod

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AGPT and HA tests were employed for rapid diagnosis of PPRV infection in sheep and goats in Sudan. Forty lymph nodes and spleen samples from suspected cases of PPR in both sheep and goats were examined by AGPT and HA tests for detection of PPRV antigen. Viral antigen was detected from (77.5%) of the samples tested by AGPT and (92.5%) tested by HA test. The results of both tests revealed that HA test was more sensitive than AGPT for detection of PPRV antigen (Kappa statistics 0.4366). Another advantage of the HA test over AGPT was that it can differentiate PPRV from RPV. Thus the HA test represents a quick, easy, simple, cheap and reliable confirmatory test for the diagnosis of PPR and differential diagnosis of PPRV and RPV. The HA test was carried out using chicken, goat and pig RBCs. Chicken RBCs were found to be the most sensitive for detection of PPRV antigen, followed by goat then pig RBCs. The HA time when using chicken RBCs was 20-25 minutes, using goat RBCs was 25-30 minutes and using pig RBCs was 40-45 minutes. The distribution of PPR infection in four different regions of Sudan was investigated.

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“…Lymphopenia is seen in case of PPR virus infection (Rajak et al, 2005). Excluding clinical signs, history and Complete blood count the PPR can also be diagnosed by using Heamagglutination test (HA), Compliment fixation test (CFT), Immuno-electro-osmo-precipitation test and Enzyme linked immuno-sorbent assay (ELISA) (Ezeibe et al, 2004).There is no specific treatment and control is possible only with vaccination.…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of Peste Des Petits in 2 Year Old Buck on The Basis of Clinical Signs and Hematology

Tariq

2013

ijmvr

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This case study signifies the diagnosis of Peste des Petits ruminants (PPR) in 2 year old buck of discrete breed on the basis of clinical signs and Hematology. Case presented to Department of Clinics Medicine and Surgery (CMS), Faculty of Veterinary Sciences (FVS) at University of Agriculture Faisalabad, Pakistan (UAF) with chief complain of anorexia, watery diarrhea, ocular discharge and muco-purulent nasal discharge from last 4 days. Clinical examination revealed high fever 104.4*F, increase in respiration and pulse rate as 38/min, and 112/min respectively. Capillary refill time was more than 3 seconds indicating dehydration (CRT >3). Lesions were visible on gums in oral cavity and lymph nodes were swollen. Complete blood count (CBC) showed lymphopenia. Disease was diagnosed as PPR on clinical basis and history. Treatment was done symptomatically for 7 days because of unavailability of specific treatment. Animal started showing recovery signs from 5 th day and recovered completely at 10 th day after visit.

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Standardisation of the haemagglutination test for peste des petits ruminants (PPR)

Cited by 17 publications

References 4 publications

Evaluation of Haemagglutination Assay (HA) for the Detection of Peste des Petits Ruminants Virus (PPRV) in Faecal Samples of Recovered Goats

Evaluation of Haemagglutination Assay (HA) for the Detection of Peste des Petits Ruminants Virus (PPRV) in Faecal Samples of Recovered Goats

Rapid detection of Peste des Petits Ruminants (PPR) virus antigen in Sudan by agar gel precipitation (AGPT) and haemagglutination (HA) Tests

Diagnosis of Peste Des Petits in 2 Year Old Buck on The Basis of Clinical Signs and Hematology

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