Standardization of incubation conditions for hemolysis testing of biomaterials

“…Some diseases and medical devices cause red blood cells to break too soon requiring the bone marrow to accelerate the regeneration of red blood cells (erythropoesis). Medical devices for hemodialysis, heart-lungbypass machines or mechanical heart valves induce more hemolysis than smaller implants like stents or catheters [201].…”

Section: Interactions Of Tissue-and Blood-cells With the Polymer Surfmentioning

confidence: 99%

Polymers in Cardiology

Sternberg¹,

Busch

Petersen

2014

Advanced Polymers in Medicine

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Polymers have found widespread applications in cardiology, in particular in coronary vascular intervention as stent platforms, coating matrices for drug-eluting stents (DES) and drug-coated balloons (DCB) and for transcatheter valve therapy. Besides permanent polymers, biodegradable polymers came in focus of current research and development for medical applications, as they degrade once their function is fulfilled, which might efficiently reduce observed hypersensitivity reactions. After reviewing polymers used for cardiovascular applications, the book chapter deals with possible surface modification reactions of the polymers including the provision with a local drug delivery function and/ or biofunctionalization in order to selectively control cell-implant interactions. These functionalizations can be furthermore designed to enhance bio-and hemocompatibility, which is of special interest for cardiovascular implants and devices. A general discussion of bio-and hemo-compatibility of polymers for cardiovascular applications and corresponding evaluation methods is additionally given. With our own published data, we finally highlight exemplary polymer applications in cardiology as polymer-based biodegradable stent platforms, biodegradable polymeric coatings for DES and hydrogel-based coatings for DCB. Moreover, developed biofunctionalization strategies of polymers are discussed with regard to their application in cardiology.

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“…Then 0.2-mL anticoagulant of rabbit blood 30 was added, and the temperature was maintained at 37 C for 60 min. To obtain the hemolysis degree, both the positive responding specimen and the negative responding specimen were prepared.…”

Section: Hemolytic Testmentioning

confidence: 99%

Anticlotting membrane based on polypropylene grafted by biocompatible monomers under UV irradiation

Zhao¹,

Wang²,

Cao³

et al. 2011

J of Applied Polymer Sci

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Biocompatible membranes were successfully prepared by biaxially oriented polypropylene (BOPP) cografted with hydroxyethyl acrylate (HEA) and vinyltriethoxysilicane (VES), in which nonvapor and nonliquid photo-grafting technology was used under the irradiation of UV lamp. HEA and VES cografted BOPP (HV-g-PP) was then used to fabricate anticlotting membrane by immobilizing heparin on it. The grafting degree of BOPP host and the chemical structure of grafting layer were studied by grafting degree test, Fourier transform infrared spectrometer (FTIR), atomic force microscope (AFM), and X-ray photoelectron spectroscopy (XPS) measurements, respectively. Anticoagulation capability of the heparin immobilized HV-g-PP membrane (HHV-g-PP) was also investigated by hemolytic test. The results show that HEA and VES can be effectively grafted onto BOPP substrates via nonvapor and nonliquid photografting method, and that the grafting degree reaches 144.8 wt % when the reaction time approaches 20 min. The heparin content of HHV-g-PP membrane increases from 0 to 4 wt % as the reaction time alters from 0 to 20 min. The hemolytic degree rapidly reduces from 5.1 wt % to 2.7 wt % with increasing the grafting degree from 0 to 144.8 wt %, implying a significant anticoagulation improvement of the HHV-g-PP membrane. Such functional membranes have large potential applications in blood-related packaging areas.

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Standardization of incubation conditions for hemolysis testing of biomaterials

Cited by 166 publications

References 11 publications

Evaluation of Docetaxel-Loaded Intravenous Lipid Emulsion: Pharmacokinetics, Tissue Distribution, Antitumor Activity, Safety and Toxicity

Evaluation of Docetaxel-Loaded Intravenous Lipid Emulsion: Pharmacokinetics, Tissue Distribution, Antitumor Activity, Safety and Toxicity

Polymers in Cardiology

Anticlotting membrane based on polypropylene grafted by biocompatible monomers under UV irradiation

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