Anticlotting membrane based on polypropylene grafted by biocompatible monomers under UV irradiation

Abstract

Biocompatible membranes were successfully prepared by biaxially oriented polypropylene (BOPP) cografted with hydroxyethyl acrylate (HEA) and vinyltriethoxysilicane (VES), in which nonvapor and nonliquid photo-grafting technology was used under the irradiation of UV lamp. HEA and VES cografted BOPP (HV-g-PP) was then used to fabricate anticlotting membrane by immobilizing heparin on it. The grafting degree of BOPP host and the chemical structure of grafting layer were studied by grafting degree test, Fourier transform infrared spectrometer (FTIR), atomic force microscope (AFM), and X-ray photoelectron spectroscopy (XPS) measurements, respectively. Anticoagulation capability of the heparin immobilized HV-g-PP membrane (HHV-g-PP) was also investigated by hemolytic test. The results show that HEA and VES can be effectively grafted onto BOPP substrates via nonvapor and nonliquid photografting method, and that the grafting degree reaches 144.8 wt % when the reaction time approaches 20 min. The heparin content of HHV-g-PP membrane increases from 0 to 4 wt % as the reaction time alters from 0 to 20 min. The hemolytic degree rapidly reduces from 5.1 wt % to 2.7 wt % with increasing the grafting degree from 0 to 144.8 wt %, implying a significant anticoagulation improvement of the HHV-g-PP membrane. Such functional membranes have large potential applications in blood-related packaging areas.

Progress in heparin and heparin-like/mimicking polymer-functionalized biomedical membranes

“…In recent years, some membrane-harmless methods, such as using UV rays and low temperature plasma (LTP), have been developed to introduce active surface groups. 62,63 Zhao et al used UV rays to prepare biocompatible biaxially oriented polypropylene (BOPP) membranes by surface co-graing with hydroxyethyl acrylate (HEA) and vinyltriethoxysilicane (VES) under the irradiation of an UV lamp. The HEA and VES co-graed BOPP (HV-g-PP) was then used to bond anticlotting heparin.…”

“…The HEA and VES co-graed BOPP (HV-g-PP) was then used to bond anticlotting heparin. 62 The signicantly enhanced anticoagulation of the heparin-graed HV-g-PP membrane exhibited great potential in applications in blood-related packaging areas. In addition, the LTP method was demonstrated to be very effective, and could reach a maximum density of peroxides and PAA when treated with LTP for only 90 s. 63 The surface plasma, irradiation, and also UV ray treatments are non-substrate dependent methods.…”

Synthesis, characterization, and self-assembly behaviors of a biodegradable and anti-clotting poly(EDTA-diol-co-butylene adipate glycol urethanes)

“…36 The anti-clotting property is also better than that of our previously reported heparin-based polypropylene membranes. 24 In addition, Fig. 8(b) reveals that the hemolysis degree of PUs dramatically reduces from 2.83% to 0.70% on increasing the EDTA-diol content from 5 to 20 wt%, and that the hemolysis degree of PUs is almost unchanged with the increase in the EDTA-diol content from 30 to 50 wt%.…”

“…23 Recently, our group has reported a novel heparin graed anti-clotting poly(propylene) (PP) membrane. 24 The results show that the blood compatibility of the PP membrane is dramatically improved aer the graing of heparin. In addition, the mechanical properties of the membrane are almost unchanged following the graing reaction, showing a large potential application in blood packaging.…”

Plasma‐Assisted Surface Modification and Heparin Immobilization: Dual‐Functionalized Blood‐Contacting Biomaterials with Improved Hemocompatibility and Antibacterial Features

“…Although heparin's presence halved the hemolysis ratio compared with unmodified PP substrates, a detailed investigation of antibacterial features and other hemocompatibility indicators has not been carried out. [ 30 ] In another study, PP surfaces were activated with a cold atmospheric plasma polymerization method to facilitate subsequent grafting of acrylic acid and polyethylene glycol. Following that, not only heparin but also chitosan and insulin were bound to the modified PP surfaces that yielded an increased hemocompatibility (decreased adhesion and activation of platelets and adsorption of plasma proteins) compared to unmodified PP.…”