Standardization of isothermal microcalorimetry in urinary tract infection detection by using artificial urine

Bonkat, Gernot; Braissant, Olivier; Rieken, Malte; Solokhina, Anna; Widmer, Andreas F.; Frei, Reno; Merwe, André van der; Wyler, Stephen; Gasser, Thomas C.; Bachmann, Alexander

doi:10.1007/s00345-012-0913-2

Cited by 24 publications

(18 citation statements)

References 27 publications

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“…In other fields of medicine, this technique has been investigated for example to be used in the screening of microorganisms in blood transfusions product 11 and in urine samples. 13 Isocalorimetry has also been used in basic research to test susceptibility of antimicrobials. 10 Microorganims exposed to bacteriostatic agents will mostly result in a lower heat production rate and microorganisms exposed to bactericidal agent will mostly result in a delayed heatflow pattern since a fraction of the inoculum is killed.…”

Section: Discussionmentioning

confidence: 99%

Accurate and early diagnosis of orthopedic device‐related infection by microbial heat production and sonication

Borens

Yusuf

Steinrücken

et al. 2013

Journal Orthopaedic Research

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Proper and rapid diagnosis of orthopedic device-related infection is important for successful treatment. Sonication has been shown to improve the diagnostic performance. We hypothesized that the combination of sonication with a novel method called microcalorimetry will further improve and accelerate the diagnosis of implant infection. We prospectively included 39 consecutive patients (mean age 59 years, 62% males) at our institution from whom 29 orthopedic prostheses and 10 osteosynthesis material were explanted. The explanted device was sonicated. The resulting sonication fluid was analyzed using microcalorimetry. Using standardized criteria to define orthopedic device-related infection, 12 cases (31%) were defined as infected. In all, positive periprosthetic tissue cultures were found. The sensitivity and specificity of microcalorimetry of sonication fluid were 100% and 97%, respectively. Mean time to detection, defined as time to reach a rising heat flow signal of 20 mW measured after equilibiration needed to get accurate measurement, was 10.9 h. In summary, microcalorimetry of sonication fluid is a reliable and a fast method in detecting the presence of microorganisms in orthopedic device-related infection. Keywords: microcalorimetry; sonication; implanted orthopedic device; infection Orthopedic implants such as joint prosthesis and fracture fixation devices are increasingly used to restore the functionality of the affected joint and the fractured bone.1,2 Unfortunately, it is estimated that 1-2% of these implants become infected.3 Orthopedic device-related infections are associated with significant morbidity and substantial health expenditures. 4 It is essential to differentiate implant-related infections from aseptic loosening because of the difference in clinical consequences. 4 However, it is difficult to make the diagnosis of implant-related infections because of variable and often subtle clinical presentation. Moreover, there are no uniform criteria for the diagnosis. 4 The diagnosis of implant related infection relies frequently on combination of clinical, imaging and histological findings, together with isolation of microorganisms from joint fluid or tissue.5 Next of being accurate, a diagnostic method for implant-related infection should be rapid in order to prompt appropriate treatment and shorten the duration of patient's morbidity.More recently, microbial cultures of samples obtained by sonication of prostheses have been shown to improve the diagnosis of prosthesic joint infection. 6 Yet, it can take up to five days before the definitive results can be released. More rapid diagnosis of implant-related infection is arguably needed in order to provide appropriate treatment.4 Isothermal microcalorimetry is a newly introduced technique in orthopedics. It can be used to detect the presence of microorganisms rapidly by measuring microbial heat production produced by microbial growth and metabolism. [7][8][9] This technique has been applied in medicine where rapid detection of microorganisms i...

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Section: Discussionmentioning

confidence: 99%

Accurate and early diagnosis of orthopedic device‐related infection by microbial heat production and sonication

Borens

Yusuf

Steinrücken

et al. 2013

Journal Orthopaedic Research

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“…3). In addition, if the sample chemical composition is unmodified or minimally modified, information on the pathogenicity of a microbe can be obtained by calculating its growth rate, for example [84,85]. This approach could easily be used for urinary tract infections where pathogens with higher growth rate in urine are considered as more aggressive [86].…”

Section: Application Of Isothermal Microcalorimetry In Clinical Settingsmentioning

confidence: 99%

Microcalorimetric assays for measuring cell growth and metabolic activity: Methodology and applications

Braissant

Bachmann

Bonkat

2015

Methods

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“…; Bonkat et al. ). Shorter lag times indicate that higher numbers of metabolically active bacteria are present in the starting inoculum.…”

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confidence: 98%

Determinants of biofilm formation and cleanability of titanium surfaces

Zaugg

Astasov‐Frauenhoffer

Braissant

et al. 2016

Clinical Oral Implants Res

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Surface hydrophilicity and roughness enhanced biofilm formation in vivo, whereas surface topography was the most influential factor that determined surface cleanability. While the grooved surface retained larger amounts of initial biofilm, the machined surface was easier to clean, but proliferation indicated by increased metabolic activity (growth rate) in IMC occurred despite mechanical biofilm removal.

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Standardization of isothermal microcalorimetry in urinary tract infection detection by using artificial urine

Cited by 24 publications

References 27 publications

Accurate and early diagnosis of orthopedic device‐related infection by microbial heat production and sonication

Accurate and early diagnosis of orthopedic device‐related infection by microbial heat production and sonication

Microcalorimetric assays for measuring cell growth and metabolic activity: Methodology and applications

Determinants of biofilm formation and cleanability of titanium surfaces

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