Standardization of the quantitative agar gel precipitin test for antibodies to infectious bursal disease

Biochemistry and Immunology of Infectious Bursal Disease Virus

“…IBDVs share common group antigens which may be detected by agar gel precipitation, the fluorescent antibody test, and ELISA (Allan et al, 1984;Jackwood et al, 1982;Hirai et al, 1972Hirai et al, , 1974Chettle et al, 1985;Cullen & Wyeth, 1975;Wood et al, 1979;Marquardt et al, 1980). Both capsid proteins (VP2 and VP3) contain epitopes that are responsible for the IBDV group antigenicity (Becht et al, 1988).…”

The interaction of live vaccine and maternal antibody in protection against infectious bursal disease

“…These were performed as described by Cullen and Wyeth (1975) on individual sera and the results were expressed in UK units per ml by standardisation with the British Standard for infectious bursal disease serum (Wood et al, 1979).…”

Quantitative counter-immunoelectrophoresis for estimation of antibodies to infectious bursal disease virus

“…Quantitative AGP was done using wells of 4 mm diameter and 2 mm interspace in 1.5 ml of 1% agarose (low EEO) gels containing 8% sodium chloride per slide (Wood et al, 1979). The results were read after 48 h incubation at room temperature in a humidified chamber.…”