Standardized test mixture for the characterization of comprehensive two-dimensional gas chromatography columns: the Phillips mix

Dimandja, Jean-Marie D.; Clouden, Garrick C.; Colón, Ivelisse; Focant, Jean‐François; Cabey, Whitney; Parry, Ritchard C.

doi:10.1016/j.chroma.2003.09.027

Cited by 48 publications

(22 citation statements)

References 27 publications

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“…Apart from the increased number of detectable peaks as compared with GC/MS, spectral purity is improved in GC×GC/TOFMS, which in turn enhances mass spectral deconvolution and compound identification. While it may be challenging to process the complex GC×GC/ TOFMS data [9,12,13], the technique has been successfully utilised in several non-targeted metabolic profiling studies [14][15][16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Metabotyping of human colorectal cancer using two-dimensional gas chromatography mass spectrometry

et al. 2012

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Colorectal cancer (CRC) is the fourth most common cause of death from cancer in the world. The limitations of the currently available methods and biomarkers for CRC management highlight the necessity of finding novel markers. Metabonomics can be used to search for potential markers that can provide molecular insight into human CRC. The emergence of two-dimensional gas chromatography time of flight mass spectrometry (GC × GC/TOFMS) has comprehensively enhanced the metabolic space coverage of conventional GC/MS. In this study, a GC × GC/TOFMS was developed for the tissue-based global metabonomic profiling of CRC. A Pegasus GC × GC/TOFMS (Leco Corp., St. Joseph, MI, USA) system comprising an Agilent 7890 GC and Pegasus IV TOFMS was used for this purpose. An Agilent DB-1 (30 m × 250 μm × 0.25 μm) fused silica capillary column and a Restek Rxi®-17 (1 m × 100 μm × 0.10 μm) fused silica capillary column were used as the primary and secondary columns, respectively. The method was applied for global metabonomic profiling of matched CRC and normal tissues (n = 63) obtained from 31 CRC patients during surgery. An attempt was also made to compare GC × GC/TOFMS with GC/MS and NMR in similar application. The results showed that the metabotype associated with CRC is distinct from that of normal tissue and led to the identification of chemically diverse marker metabolites. Metabolic pathway mapping suggested deregulation of various biochemical processes such as glycolysis, Krebs cycle, osmoregulation, steroid biosynthesis, eicosanoid biosynthesis, bile acid biosynthesis, lipid, amino acid and nucleotide metabolism.

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Section: Introductionmentioning

confidence: 99%

Metabotyping of human colorectal cancer using two-dimensional gas chromatography mass spectrometry

et al. 2012

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“…Between the two columns is a thermal modulator (Dimandja et al, 2003;Focant et al, 2003Focant et al, , 2004cSemard et al, 2009) which traps portions of eluent from the first column and re-injects them onto the second column. This process occurs repeatedly, thus all the compounds from the sample are subjected to two distinct separations, resulting in a separation superior to that possible on a 1 D GC system (Schoenmakers et al, 2003), with many more compounds resolved as a result of the multiplicative increase in peak separation number.…”

Section: Introductionmentioning

confidence: 99%

Comprehensive two-dimensional gas chromatography time of flight mass spectrometry (GC×GC-TOFMS) for environmental forensic investigations in developing countries

Vos

Dixon²,

Vermeulen³

et al. 2011

Chemosphere

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a b s t r a c tThe disposal and dumping of toxic waste is a matter of growing concern in developing countries, including South Africa. Frequently these countries do not possess access to gas chromatography-high resolution mass spectrometry (GC-HRMS) for the determination of persistent organic pollutants (POPs). This publication describes an alternative approach to the investigation of toxic waste using comprehensive gas chromatography coupled to time of flight mass spectrometry (GC Â GC-TOFMS). The technology permits both comprehensive screening of toxic samples for numerous classes of organic pollutants and also quantitative analysis for the individual compounds. This paper describes the use of this technique by analysing samples obtained from a hazardous waste treatment facility in South Africa. After sampling and extraction the samples were analysed for polychlorinated dibenzo-p-dioxins (PCDDs), polychlorinated dibenzofurans (PCDFs) and four dioxin-like non-ortho substituted polychlorinated biphenyls (PCBs). The quantitative values, as well as detection limits, obtained using the GC Â GC-TOFMS methodology compares well with those obtained using GC-HRMS; the accepted benchmark technology for this analysis. Although GC Â GC-TOFMS is not a target compound analytical technique (as is GC-HRMS), it is possible to obtain information on numerous other classes of organic pollutants present in the samples in one analytical run. This is not possible with GC-HRMS. Several different column combinations have been investigated for handling very complex waste samples and suggestions are presented for the most suitable combination.

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“…Because of the fast and, thus, essentially isothermal seconddimension separation, for such analytes of equal volatility there will be no boiling point contribution in that dimension: only the specific interactions will govern analyte retention. In other words, the two dimensions operate statistically independently: the separation is therefore orthogonal and the entire 2D plane of the GCÂGC chromatogram, the separation space, is available for peak separation [18,19]. One main benefit of orthogonal separations is that ordered structuresi.e.…”

Section: Instrumental Set-upmentioning

confidence: 99%

Multidimensional and Comprehensive Two-Dimensional Gas Chromatography

Adahchour

Brinkman

2014

Practical Gas Chromatography

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Standardized test mixture for the characterization of comprehensive two-dimensional gas chromatography columns: the Phillips mix

Cited by 48 publications

References 27 publications

Metabotyping of human colorectal cancer using two-dimensional gas chromatography mass spectrometry

Metabotyping of human colorectal cancer using two-dimensional gas chromatography mass spectrometry

Comprehensive two-dimensional gas chromatography time of flight mass spectrometry (GC×GC-TOFMS) for environmental forensic investigations in developing countries

Multidimensional and Comprehensive Two-Dimensional Gas Chromatography

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