Staphylococcal bovine mastitis in France: enterotoxins, resistance and the human Geraldine methicillin-resistant Staphylococcus aureus clone

Haenni, Marisa; Galofaro, Laure; Ponsin, Cécile; Bes, Michèle; Laurent, François; Madec, Jean‐Yves

doi:10.1093/jac/dkq417

Cited by 25 publications

(18 citation statements)

References 10 publications

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“…Although we did not assess the presence of the toxins directly in food, our study found a very high frequency (93.6%) of S. aureus isolates harboring one or more SE genes; whereas, in some recent studies this proportion ranged from 37 to 69% (2,12,18,26,36). According to Zadoks and coworkers (53), the detection of staphylococcal enterotoxin may be important to public health because of its potential to cause food poisoning or clinical syndromes such as toxic shock syndrome, but apparently this is not essential for the pathogenesis of bovine mastitis.…”

Section: Discussioncontrasting

confidence: 76%

“…A high resistance profile was found in the region; each dairy farm had its own particular resistance profile, regardless of genotypic profile. Despite the increasing number of publications on methicillin-resistant S. aureus responsible for infections in animals, these have only occasionally been found in bovine mastitis (18,27,31). Earlier work by our group (44) did not identify the mecA gene among S. aureus resistant to oxacillin (methicillin) in our region (herds 3 and 4).…”

Section: Discussionmentioning

confidence: 69%

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Antibiotic Resistance and Molecular Analysis of Staphylococcus aureus Isolated from Cow's Milk and Dairy Products in Northeast Brazil

Silveira-Filho¹,

Luz²,

Campos³

et al. 2014

Journal of Food Protection

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This work aimed to assess the clonal distribution among 94 strains of Staphylococcus aureus isolated from cow's milk, raw cheese, and a milking machine in 12 dairy farms in northeast Brazil, by analyzing different typing methods and detecting resistance and toxigenic profiles. For the first time, isolates of this region were assessed simultaneously by the polymorphism of the 3'-end coa gene and 16S-23S rDNA, pulsed-field gel electrophoresis, antibiotic resistance phenotyping, and toxigenic arsenal. Although pulsed-field gel electrophoresis patterns showed a wider variation (discriminatory index 0.83) than the PCR-based methods, the internal transcribed spacer-PCR proved to be a useful and inexpensive procedure for conducting epidemiological surveys of S. aureus on a regional scale. Each dairy farm had its own resistance profile, and in two herds, 63% of the strains were multiresistant, probably due to the indiscriminate use of antibiotics in bovine mastitis treatment. No methicillin-resistant S. aureus strains were detected in this study; however, 93.6% of S. aureus strains harbored variable profiles of staphylococcal enterotoxin genes seg, seh, sei, and sej. Transcriptional analysis revealed that 53.3% of staphylococcal enterotoxin genes actually transcribed, pointing out the food poisoning risk of these dairy products to consumers in the region. Based on the detection of the most prevalent clones in a herd or region, appropriate antibiotic therapy and specific immunization can be used for the treatment and control of staphylococcal mastitis.

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Section: Discussioncontrasting

confidence: 76%

Section: Discussionmentioning

confidence: 69%

Antibiotic Resistance and Molecular Analysis of Staphylococcus aureus Isolated from Cow's Milk and Dairy Products in Northeast Brazil

Silveira-Filho¹,

Luz²,

Campos³

et al. 2014

Journal of Food Protection

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“…S. aureus strains carrying enterotoxin genes have been isolated from a variety of foods, often from dairy products [17]. One important source of dairy product contamination is in fact a veterinary problem: mastitis caused by this pathogen or poor hygiene in processing dairy products [18]. The presence of enterotoxigenic S. aureus in both raw milk and dairy products results in staphylococcal food poisoning (SFP) [18].…”

Section: Staphylococcus Sppmentioning

confidence: 99%

Facing Antibiotic Resistance: Staphylococcus aureus Phages as a Medical Tool

Kaźmierczak

Górski

Dąbrowska

2014

Viruses

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Staphylococcus aureus is a common and often virulent pathogen in humans. This bacterium is widespread, being present on the skin and in the nose of healthy people. Staphylococcus aureus can cause infections with severe outcomes ranging from pustules to sepsis and death. The introduction of antibiotics led to a general belief that the problem of bacterial infections would be solved. Nonetheless, pathogens including staphylococci have evolved mechanisms of drug resistance. Among current attempts to address this problem, phage therapy offers a promising alternative to combat staphylococcal infections. Here, we present an overview of current knowledge on staphylococcal infections and bacteriophages able to kill Staphylococcus, including experimental studies and available data on their clinical use.

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“…It is also known to be contagious, spreading in a farm from cow to cow during the milking process by contaminated machines, equipments, or milkers' hands. The presence of such pathogens in foodproducing animals or in raw milk raises the question of the potential transmissibility of specific resistant clones or antimicrobial resistance determinants, as well as the possible routes of transmission from the animal to humans or vice versa (5,7,10).…”

mentioning

confidence: 99%

Link between Genotype and Antimicrobial Resistance in Bovine Mastitis-Related Staphylococcus aureus Strains, Determined by Comparing Swiss and French Isolates from the Rhône Valley

Sakwińska

Morisset

Madec³

et al. 2011

Appl Environ Microbiol

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Staphylococcus aureus is a major bovine mastitis pathogen. Although the reported antimicrobial resistance was generally low, the emergence of new genetic clusters in bovine mastitis requires examination of the link between antimicrobial resistance and genotypes. Here, amplified fragment length polymorphism (AFLP) profiles and standard antimicrobial resistance profiles were determined in order to characterize a total of 343 S. aureus cow mastitis isolates from two geographically close regions of Switzerland and France. AFLP profiles revealed similar population compositions in the two regions, with 4 major clusters (C8, C20, C97, and C151), but the proportions of isolates in each cluster significantly diverged between the two countries (P ‫؍‬ 9.2 ؋ 10 ؊9 ). Antimicrobial resistance was overall low (<5% resistance to all therapeutically relevant molecules), with the exception of penicillin resistance, which was detected in 26% of the isolates. Penicillin resistance proportions differed between clusters, with only 1 to 2% of resistance associated with C20 and C151 and up to 70% associated with bovine C97. The prevalence of C20 and C8 was unexpectedly high and requires further investigation into the mechanism of adaptation to the bovine host. The strong association of penicillin resistance with few clusters highlights the fact that the knowledge of local epidemiology is essential for rational choices of antimicrobial treatment in the absence of susceptibility testing. Taken together, these observations argue in favor of more routine scrutiny of antimicrobial resistance and antibiotic-resistant clones in cattle and the farm environment.

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Staphylococcal bovine mastitis in France: enterotoxins, resistance and the human Geraldine methicillin-resistant Staphylococcus aureus clone

Cited by 25 publications

References 10 publications

Antibiotic Resistance and Molecular Analysis of Staphylococcus aureus Isolated from Cow's Milk and Dairy Products in Northeast Brazil

Antibiotic Resistance and Molecular Analysis of Staphylococcus aureus Isolated from Cow's Milk and Dairy Products in Northeast Brazil

Facing Antibiotic Resistance: Staphylococcus aureus Phages as a Medical Tool

Link between Genotype and Antimicrobial Resistance in Bovine Mastitis-Related Staphylococcus aureus Strains, Determined by Comparing Swiss and French Isolates from the Rhône Valley

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