Link between Genotype and Antimicrobial Resistance in Bovine Mastitis-Related Staphylococcus aureus Strains, Determined by Comparing Swiss and French Isolates from the Rhône Valley

Sakwińska, Olga; Morisset, Delphine; Madec, Jean‐Yves; Waldvogel, Andreas; Moreillon, Philippe; Haenni, Marisa

doi:10.1128/aem.02468-10

Cited by 40 publications

(48 citation statements)

References 30 publications

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“…These findings demonstrate that bovine strains of Staph. aureus are clonal; this observation is not new (van den Borne et al, 2010;Sakwinska et al, 2011;Kock et al, 2013;Vandendriessche et al, 2014). A new finding of the present study, however, is that the spectrum of CC associated with bovine IMI is considerably broader than expected from the cited publications: in addition to the 6 major known CC, 20 further CC were observed.…”

Section: Clonality Of Bovine Strains and Phylogeneticscontrasting

confidence: 48%

“…Types t1403 and t524 are absent in the SRL maps database, and t267 is rare. All t267-positive strains were positive for CC97, a CC that is highly associated with cattle (Rabello et al, 2007;Sakwinska et al, 2011;Vandendriessche et al, 2014) but has rarely been described as a pathogen of a human infection (Menegotto et al, 2012). In the case of t1403, most were CC133-positive and the remainder were CC97-positive.…”

Section: Zoonotic Transfer Of Staph Aureus Between Cattle and Humansmentioning

confidence: 99%

“…Staphylococcus aureus has been extensively studied and various typing methods have been described for subtyping this pathogen (Rabello et al, 2007;Hwang et al, 2010;van den Borne et al, 2010;Sakwinska et al, 2011). Two common methods are spa typing (Harmsen et al, 2003) and multilocus sequence typing (MLST; Enright et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

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Bovine Staphylococcus aureus: Subtyping, evolution, and zoonotic transfer

Boss

Cosandey

Luini

et al. 2016

Journal of Dairy Science

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Staphylococcus aureus is globally one of the most important pathogens causing contagious mastitis in cattle. Previous studies using ribosomal spacer (RS)-PCR, however, demonstrated in Swiss cows that Staph. aureus isolated from bovine intramammary infections are genetically heterogeneous, with Staph. aureus genotype B (GTB) and GTC being the most prominent genotypes. Furthermore, Staph. aureus GTB was found to be contagious, whereas Staph. aureus GTC and all the remaining genotypes were involved in individual cow disease. In addition to RS-PCR, other methods for subtyping Staph. aureus are known, including spa typing and multilocus sequence typing (MLST). They are based on sequencing the spa and various housekeeping genes, respectively. The aim of the present study was to compare the 3 analytic methods using 456 strains of Staph. aureus isolated from milk of bovine intramammary infections and bulk tanks obtained from 12 European countries. Furthermore, the phylogeny of animal Staph. aureus was inferred and the zoonotic transfer of Staph. aureus between cattle and humans was studied. The analyzed strains could be grouped into 6 genotypic clusters, with CLB, CLC, and CLR being the most prominent ones. Comparing the 3 subtyping methods, RS-PCR showed the highest resolution, followed by spa typing and MLST. We found associations among the methods but in many cases they were unsatisfactory except for CLB and CLC. Cluster CLB was positive for clonal complex (CC)8 in 99% of the cases and typically positive for t2953; it is the cattle-adapted form of CC8. Cluster CLC was always positive for t529 and typically positive for CC705. For CLR and the remaining subtypes, links among the 3 methods were generally poor. Bovine Staph. aureus is highly clonal and a few clones predominate. Animal Staph. aureus always evolve from human strains, such that every human strain may be the ancestor of a novel animal-adapted strain. The zoonotic transfer of IMI-and milk-associated strains of Staph. aureus between cattle and humans seems to be very limited and different hosts are not considered as a source for mutual, spontaneous infections. Spillover events, however, may happen.

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Section: Clonality Of Bovine Strains and Phylogeneticscontrasting

confidence: 48%

Section: Zoonotic Transfer Of Staph Aureus Between Cattle and Humansmentioning

confidence: 99%

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Bovine Staphylococcus aureus: Subtyping, evolution, and zoonotic transfer

Boss

Cosandey

Luini

et al. 2016

Journal of Dairy Science

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“…Clustering of AFLP data and MLST genotyping of several isolates per AFLP cluster were used to establish the identity of AFLP clusters as previously described (30,32). The genotype composition of S. aureus isolates from pigs and pig farmers and veterinarians was compared to those obtained earlier for healthy volunteers without contact with agriculture and from cow farmers (30,31).…”

Section: Resultsmentioning

confidence: 99%

Antimicrobial Resistance of Staphylococcus aureus Strains Acquired by Pig Farmers from Pigs

Oppliger

Moreillon

Charrière

et al. 2012

Appl Environ Microbiol

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bCarriage of animal-associated methicillin-resistant Staphylococcus aureus (MRSA) clonal complex 398 (CC398) is common among pig farmers. This study was conducted (i) to investigate whether pig farmers are colonized with pig-specific S. aureus genotypes other than CC398 and (ii) to survey antimicrobial resistance of S. aureus isolates from pigs and pig farmers. Fortyeight S. aureus isolates from pig farmers and veterinarians and 130 isolates from pigs collected in Western Switzerland were genotyped by spa typing and amplified fragment length polymorphism (AFLP). Antimicrobial resistance profiles were determined for representative sample of the isolates. The data obtained earlier on healthy S. aureus carriers without exposure to agriculture were used for comparison. The genotype composition of S. aureus isolates from pig farmers and veterinarians was similar to isolates from pigs with predominant AFLP clusters CC398, CC9, and CC49. The resistance to tetracycline and macrolides (clarithromycin) was common among the isolates from farmers and veterinarians (52 and 21%, respectively) and similar to resistance levels in isolates from pigs (39 and 23%, respectively). This was in contrast to isolates from persons without contact with agriculture, where no (0/128) isolates were resistant to tetracycline and 3% of the isolates were resistant to clarithromycin. MRSA CC398 was isolated from pigs (n ‫؍‬ 11) and pig farmers (n ‫؍‬ 5). These data imply that zoonotic transmission of multidrug-resistant S. aureus from pigs to farmers is frequent, and well-known MRSA transmission merely represents the tip of the iceberg for this phenomenon. We speculate that the relatively low frequency of MRSA isolation is related to lower antimicrobial use in Switzerland compared to, for example, the Netherlands.

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“…The link between genotype and AMR in S. aureusassociated bovine mastitis was studied by Sakwinska et al (22) and their observations revealed a strong association of penicillin-resistant clusters, highlighting the fact that knowledge of local epidemiology is essential for accurate treatment by veterinarians. They further emphasized the need of more research and knowledge of local and global epidemiology of S. aureus clones.…”

Section: Discussionmentioning

confidence: 99%