Staphylococcal IgM Enzyme-Linked Immunosorbent Assay for Diagnosis of Periprosthetic Joint Infections

Artini, Marco; Romanò, Carlo Luca; Manzoli, Lamberto; Scoarughi, Gian Luca; Papa, Rosanna; Meani, E.; Drago, Lorenzo; Selan, Laura

doi:10.1128/jcm.01836-10

Cited by 25 publications

(16 citation statements)

References 10 publications

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“…60 Researchers are investigating the potential for measuring serum antibody to staphylococcal slime polysaccharide antigens as a screening test for underlying device-associated infection. 61 A recent study has identified the use of infection-specific radiotracers such as bacteriophages and thymidine kinase in conjunction with single-photon emission computed tomography and positron emission tomography scans to diagnose biofilm in orthopedic prostheses. 34 Further investigation of these modalities is warranted.…”

Section: Prosthetic Jointsmentioning

confidence: 99%

The Role of Bacterial Biofilms in Device-Associated Infection

Deva

Adams

Vickery

2013

Plastic and Reconstructive Surgery

226

121

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There is increasing evidence that bacterial biofilm is responsible for the failure of medical devices, leading to device-associated infection. As plastic surgeons, we are among the leading users of prostheses in surgery, and it is important that we are kept informed of this growing problem. This article summarizes the pathogenesis of device-associated infection, outlines the evidence for such infection in a number of medical devices, and outlines operative strategies aimed at reducing the risk of bacterial contamination at the time of device deployment. It also outlines strategies under investigation to combat the development of device-associated infection.

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Section: Prosthetic Jointsmentioning

confidence: 99%

The Role of Bacterial Biofilms in Device-Associated Infection

Deva

Adams

Vickery

2013

Plastic and Reconstructive Surgery

226

121

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“…Quantification of in vitro biofilm production was based on the method described by Christensen with slight modifications. 18 Briefly, the wells of a sterile 48-well flat-bottomed polystyrene plate were filled with 400 μL of BHI medium; 1/100 dilution of overnight bacterial cultures was added to each well. The first row contained the untreated bacteria, while each of the remaining rows contained serial dilutions of supernatant (SN) starting from 1:2.…”

Section: Biofilm Formation Of Staphylococcimentioning

confidence: 99%

Anti-biofilm activity of pseudoalteromonas haloplanktis tac125 against staphylococcus epidermidis biofilm: Evidence of a signal molecule involvement?

Parrilli

Papa²,

Carillo

et al. 2015

Int J Immunopathol Pharmacol

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Staphylococcus epidermidis is recognized as cause of biofilm-associated infections and interest in the development of new approaches for S. epidermidis biofilm treatment has increased. In a previous paper we reported that the supernatant of Antarctic bacterium Pseudoalteromonas haloplanktis TAC125 presents an anti-biofilm activity against S. epidermidis and preliminary physico-chemical characterization of the supernatant suggested that this activity is due to a polysaccharide. In this work we further investigated the chemical nature of the anti-biofilm P. haloplanktis TAC125 molecule. The production of the molecule was evaluated in different conditions, and reported data demonstrated that it is produced in all P. haloplanktis TAC125 biofilm growth stages, also in minimal medium and at different temperatures. By using a surface coating assay, the surfactant nature of the anti-biofilm compound was excluded. Moreover, a purification procedure was set up and the analysis of an enriched fraction demonstrated that the anti-biofilm activity is not due to a polysaccharide molecule but that it is due to small hydrophobic molecules that likely work as signal. The enriched fraction was also used to evaluate the effect on S. epidermidis biofilm formation in dynamic condition by BioFlux system.

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“…49,51,54,55 Furthermore, detection of serum IgM against Staphylococcal slime polysaccharide antigens was used recently for diagnosis of staphylococcal periprosthetic joint infections with 89.7% sensitivity and 95.1% specificity. 51,56 Sometimes blood leukocyte count, Creactive protein, interleukin-6 and procalcitonin also give indications. 51 The combination of microbiological routine and new methods, together with clinical symptoms and blood inflammatory markers, will give us a better picture of orthopedic implant infections.…”

Section: Diagnosis Of the Prosthesis-related Infectionsmentioning

confidence: 99%

Prosthesis infections after orthopedic joint replacement: the possible role of bacterial biofilms

et al. 2013

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Prosthesis-related infection is a serious complication for patients after orthopedic joint replacement, which is currently difficult to treat with antibiotic therapy. Consequently, in most cases, removal of the infected prosthesis is the only solution to cure the infection. It is, therefore, important to understand the comprehensive interaction between the microbiological situation and the host immune responses that lead to prosthesis infections. Evidence indicates that prosthesis infections are actually biofilm-correlated infections that are highly resistant to antibiotic treatment and the host immune responses. The authors reviewed the related literature in the context of their clinical experience, and discussed the possible etiology and mechanism leading to the infections, especially problems related to bacterial biofilm, and prophylaxis and treatment of infection, including both microbiological and surgical measures. Recent progress in research into bacterial biofilm and possible future treatment options of prosthesis-related infections are discussed.

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Staphylococcal IgM Enzyme-Linked Immunosorbent Assay for Diagnosis of Periprosthetic Joint Infections

Cited by 25 publications

References 10 publications

The Role of Bacterial Biofilms in Device-Associated Infection

The Role of Bacterial Biofilms in Device-Associated Infection

Anti-biofilm activity of pseudoalteromonas haloplanktis tac125 against staphylococcus epidermidis biofilm: Evidence of a signal molecule involvement?

Prosthesis infections after orthopedic joint replacement: the possible role of bacterial biofilms

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