Staphylococcus aureus impairs cutaneous wound healing by activating the expression of a gap junction protein, connexin-43 in keratinocytes

Xu, Wei; Dielubanza, Elodi; Maisel, Amanda; Leung, Kai P.; Mustoe, Thomas A.; Hong, Seok Jong; Galiano, Robert D.

doi:10.1007/s00018-020-03545-4

Cited by 17 publications

(20 citation statements)

References 57 publications

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“…Considering that the healing process could be affected also by the presence of bacteria, TH1-3 formulations were further evaluated for their antimicrobial activity against S. aureus , P. aeruginosa , and S. epidermidis . S. aureus , and epidermidis were selected as prototypes of Gram-positive and negative bacteria, respectively, and are notoriously resistant to therapeutics, which frequently colonize chronic wounds [ 32 , 33 , 34 ]. The choice of the selected microorganisms also took into account their prevalence in the various stages of the wound.…”

Section: Resultsmentioning

confidence: 99%

“…The choice of the selected microorganisms also took into account their prevalence in the various stages of the wound. Indeed, there is evidence that in an early phase of the chronic wound, S. aureus , including methicillin resistant S. aureus (MRSA), are predominant, while, in the advanced stages, Gram-negative bacteria, including Pseudomonas varieties, are recognized as responsible for a penetration of the profounder layers of skin, producing significant tissue injury [ 31 , 33 ]. In time-kill assays, a reduction in the CFU was observed after 8 h when bacteria were grown in the presence of HAL and TH1-3 .…”

Section: Resultsmentioning

confidence: 99%

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In Vitro Wound-Healing Properties of Water-Soluble Terpenoids Loaded on Halloysite Clay

et al. 2021

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Recently, mineral healing clays have gained much attention for wound-dressing applications. Here, we selected halloysite (HAL) clay as a biocompatible, non-toxic material that is useful as a drug delivery system to enhance the healing properties of water-soluble terpenoids 1-3 (T1-3). Terpenoids-loaded HAL clay (TH1-3) was prepared and characterized by adsorption equilibrium studies, X-ray powder diffraction (XRPD), thermogravimetric analysis (TGA), differential scanning calorimetry (DSC), Fourier-transform infrared (FTIR) spectroscopy, and release studies. The results reveal that T1-3 were adsorbed at the HAL surface with good efficiency. The prevalent mechanism of drug retention is due to the adsorption via electrostatic interactions between the cationic groups of the T1-3 and the HAL’s external surface. Release studies demonstrated that T3 was released in a higher percentage (>60%) compared to T1-2 (≈50%). Additionally, TH1-3 were assessed for their antimicrobial activity and capability to promote the re-epithelialization of scratched HaCat monolayers, through the time-kill test and the wound-healing assays, respectively. The results reveal that all the tested formulations were able to reduce the microbial growth after 1 h of incubation and that they ensured complete wound closure after 48 h. Furthermore, at the concentration of 1 µg/mL, TH3 exhibited 45% wound closure at 24 h, compared to TH1 (27%) and TH2 (30%), proving to be the best candidate in making the tissue-repair process easier and faster.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

In Vitro Wound-Healing Properties of Water-Soluble Terpenoids Loaded on Halloysite Clay

et al. 2021

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Section: Connexin43 In Chronic Woundsmentioning

confidence: 99%

“…when S. aureus exudate was used on HaCat cells to understand what pathways were involved [161]. Cx43 was found to be upregulated in migrating HaCat cells exposed to S. aureus exudate and showed slower migration [161]. Furthermore, Akt and ERK1/2 showed upregulated phosphorylation levels during HaCat cell migration and when Akt phosphorylation was inhibited, wound healing was accelerated [161].…”

Section: Connexin43 In Chronic Woundsmentioning

confidence: 99%

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Towards the prevention of pressure ulcers

Kwek¹

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Adhesive, Flexible, and Fast Degradable 3D‐Printed Wound Dressings with a Simple Composition

Hu,

Tang,

et al. 2023

Adv Healthcare Materials

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Abstract3D printing technology has revolutionized the field of wound dressings, offering tailored solutions with mechanical support to facilitate wound closure. In addition to personalization, the intricate nature of the wound healing process requires wound dressing materials with diverse properties, such as moisturization, flexibility, adhesion, anti‐oxidation and degradability. Unfortunately, current materials used in digital light processing (DLP) 3D printing have been inadequate in meeting these crucial criteria. This study introduces a novel DLP resin that is biocompatible and consists of only three commonly employed non‐toxic compounds in biomaterials, i.e., dopamine, poly(ethylene glycol) diacrylate, and N‐vinylpyrrolidone. Simple as it is, this material system fulfills all essential functions for effective wound healing. Unlike most DLP resins that are non‐degradable and rigid, our material exhibits tunable and rapid degradation kinetics, allowing for complete hydrolysis within a few hours. Furthermore, the high flexibility enables conformal application of complex dressings in challenging areas such as finger joints. Using a difficult‐to‐heal wound model, we substantiate the manifold positive effects on wound healing in vivo, including granulation tissue formation, inflammation regulation, and vascularization. The simplicity and versatility of our material make it a promising option for personalized wound care, holding significant potential for future translation.This article is protected by copyright. All rights reserved

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Staphylococcus aureus impairs cutaneous wound healing by activating the expression of a gap junction protein, connexin-43 in keratinocytes

Cited by 17 publications

References 57 publications

In Vitro Wound-Healing Properties of Water-Soluble Terpenoids Loaded on Halloysite Clay

In Vitro Wound-Healing Properties of Water-Soluble Terpenoids Loaded on Halloysite Clay

Towards the prevention of pressure ulcers

Adhesive, Flexible, and Fast Degradable 3D‐Printed Wound Dressings with a Simple Composition

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