Staphylococcus aureus of bovine origin: Genetic diversity, prevalence and the expression of adhesin-encoding genes

Klein, Raphael Contelli; Fabres-Klein, Mary Hellen; Brito, Maria Aparecida Vasconcelos Paiva; Fietto, Luciano Gomes; Ribon, Andréa de Oliveira Barros

doi:10.1016/j.vetmic.2012.05.025

Cited by 39 publications

(40 citation statements)

References 23 publications

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“…The clumping factors play a critical role in attachment and colonization of S. aureus in body surfaces, such as skin surface and epithelium. Similar to our present study, Raphael detected clfB in 91.8% of the isolates (29). In the previous studies, the prevalence of these genes was similarly high (30,31).…”

Section: Discussionsupporting

confidence: 91%

High Frequency of icaAD, clumping factors A/B, fib and eno Genes in Staphylococcus aureus Species Isolated From Wounds in Tehran, Iran during 2012-2013

Ghasemian

Peerayeh

Bakhshi

et al. 2015

Arch Clin Infect Dis

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Background: Staphylococcus aureus (S. aureus) isolates have emerged in healthcare and community, and cause a wide spectrum of clinical signs. On the other hand, methicilin-resistant S. aureus (MRSA) isolates can resist a wide range of antibiotics, which can make treatment of infections much more difficult. Objectives: The current study aimed to determine S. aureus characteristics isolated from wounds. Materials and Methods: This definitive and cross-sectional study was conducted in Tehran, Iran. A total of 15 S. aureus isolates were collected from wound samples with sterile swabs and were identified by the conventional diagnostic tests. The antibiotic susceptibility pattern was performed according to the Clinical and Laboratory Standards Institute (CLSI, 2012) protocol. The mecA gene, Staphylococcal Cassette Chromosome mec (SCCmec) types, agr specific groups and biofilm related genes were detected by Polymerase Chain Reaction (PCR) assay. Results: The majority of the isolates were resistant to amoxicillin, tetracycline and erythromycin, but all were susceptible to vancomycin and linezolid. Eight (40%) isolates were methicillin resistant and the mecA gene was detected in these isolates. The majority of (90%) of MRSA harbored SCCmec type III, and two isolates harbored type V. The majority (70%, n = 14) of the isolates belonged to agr I, followed by agr II (15%, n = 3), agr IV (10%, n = 2) and agr III (5%, n = 1). The frequencies of clfAB, fnbAB, fib, eno, cna, ebps and bbp genes were 100%, 100%, 65% (n = 13), 55% (n = 11), 70% (n = 14), 70% (n = 14), 55% (n = 11), 0% and 0%, respectively. Conclusions: Half of the isolates were MRSA, the majority of which harbored SCCmec type III. Moreover, most of S. aureus isolates belonged to agrI. The frequency of icaAD, clfAB, fib and eno genes were high in S. aureus species isolated from wounds.

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Section: Discussionsupporting

confidence: 91%

High Frequency of icaAD, clumping factors A/B, fib and eno Genes in Staphylococcus aureus Species Isolated From Wounds in Tehran, Iran during 2012-2013

Ghasemian

Peerayeh

Bakhshi

et al. 2015

Arch Clin Infect Dis

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“…The cna gene is considered an important virulence factor in staphylococcal infections [34]. The incidence of cna was 11.32% in our isolates.…”

Section: Discussionmentioning

confidence: 63%

Genes encoding adhesion factors and biofilm formation in methicillin-resistant Staphylococcus aureus in Morocco

Serray

Oufrid

Hannaoui

et al. 2016

J Infect Dev Ctries

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Introduction: Infections involving methicillin-resistant Staphylococcus aureus (MRSA) remain a serious threat to hospitalized patients worldwide. MRSA is characterized by recalcitrance to antimicrobial therapy, which is a function not only of widespread antimicrobial resistance, but also the capacity to form biofilms. The present study evaluated the presence of genes encoding adhesion factors and the biofilmforming capacity in MRSA. Methodology: In this study, 53 isolates of MRSA, recovered from December 2010 to May 2014 in a mother and child hospital, CHU Mohamed VI in Marrakech, Morocco, were screened for the presence of bap and ica genes associated with biofilm formation, and for bbp, cna, ebpS, eno, fib, fnbA, fnbB, clfA, and clfB genes that encode microbial surface components recognizing adhesive matrix molecules (MSCRAMMs). The biofilm formation assay was performed in 96-well microtiter polystyrene plates. The presence of genes was determined by polymerase chain reaction (PCR). Results: An association was found between icaD gene detection and biofilm formation; 100% of the strains harbored icaD and produced biofilm. None of the isolates harbored bap or bbp. Furthermore, 96.23% isolates were positive for fnbA, 60.37% for eno, 43.39% for clfA and clfB, 11.32% for cna, 9.34% for ebpS, 5.66% for fib, and 1.89% for fnbA. Conclusions: Our findings showed that the MRSA carriage in Marrakech children was high. The genetic variations of adhesion genes require further investigation.

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“…Interestingly, LP genes were mostly allelic variants only found in MRSA strains: this finding is in accordance with a recent paper [24] demonstrating that livestock MRSA are typically not diffusive. Regarding the carriage of cna by LP strains, the gene has been suggested not to play an important role in S. aureus intramammary infections [26]. Different genes were significantly related to the strains isolated in those herds, where the prevalence of S. aureus mastitis was above 5%.…”

Section: Discussionmentioning

confidence: 99%

“…Sed , ser , and sej belong to a cluster harbored by different CCs, out of them CC151 and CC479 were indicated as the most frequent ones [11]. The genes sea , sak and scn , which are carried by β-hemolysin-converting bacteriophages, were present uniquely in some CC8 strains; their prevalence was higher than in other studies on bovine isolates, although lower than in studies on isolates from humans [20,21,22,23,24,25,26,27]. Accordingly, a recent paper reported that CC8 strains of bovine or human origin differed for the mobile genetic elements, among them the β-hemolysin-converting prophages: all bovine-only isolates were devoid of such prophages [28], probably because the untruncated hlb is necessary in ungulates for the different structure of erythrocyte membranes.…”

Section: Discussionmentioning

confidence: 99%

Virulence Genes of S. aureus from Dairy Cow Mastitis and Contagiousness Risk

Magro

Biffani²,

Minozzi

et al. 2017

Toxins

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Staphylococcus aureus (S. aureus) is a major agent of dairy cow intramammary infections: the different prevalences of mastitis reported might be related to a combination of S. aureus virulence factors beyond host factors. The present study considered 169 isolates from different Italian dairy herds that were classified into four groups based on the prevalence of S. aureus infection at the first testing: low prevalence (LP), medium–low (MLP), medium–high (MHP) and high (HP). We aimed to correlate the presence of virulence genes with the prevalence of intramammary infections in order to develop new strategies for the control of S. aureus mastitis. Microarray data were statistically evaluated using binary logistic regression and correspondence analysis to screen the risk factors and the relationship between prevalence group and gene. The analysis showed: (1) 24 genes at significant risk of being detected in all the herds with infection prevalence >5%, including genes belonging to microbial surface components recognizing adhesive matrix molecules (MSCRAMMs), immune evasion and serine proteases; and (2) a significant correlation coefficient between the genes interacting with the host immune response and HP isolates against LP ones. These results support the hypothesis that virulence factors, in addition to cow management, could be related to strain contagiousness, offering new insights into vaccine development.

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Staphylococcus aureus of bovine origin: Genetic diversity, prevalence and the expression of adhesin-encoding genes

Cited by 39 publications

References 23 publications

High Frequency of icaAD, clumping factors A/B, fib and eno Genes in Staphylococcus aureus Species Isolated From Wounds in Tehran, Iran during 2012-2013

High Frequency of icaAD, clumping factors A/B, fib and eno Genes in Staphylococcus aureus Species Isolated From Wounds in Tehran, Iran during 2012-2013

Genes encoding adhesion factors and biofilm formation in methicillin-resistant Staphylococcus aureus in Morocco

Virulence Genes of S. aureus from Dairy Cow Mastitis and Contagiousness Risk

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