Staphylococcus Aureus Resistentes a Meticilina E Meropenem Em Leite De Vacas Com Mastite Subclínica/ Methicillin and Meropenem Resistant Staphylococcus Aureus in Milk of Cows With Subclinical Mastitis

Souza, Geziella Áurea Aparecida Damasceno; Carvalho, Carolina Magalhães Caires; Xavier, Ester Dias; Borges, Laura Francielle Ferreira; Gonçalves, Samuel Ferreira; Almeida, Anna Christina de

doi:10.34117/bjdv6n12-340

Cited by 1 publication

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“…These findings suggest that the treatment averages are statistically distinct from each other. Damasceno [25], in his research, isolated a total of 79 strains of Staphylococcus aureus, which accounted for 36.9% of the total isolates. The study demonstrated that among these isolates, 63.3% (n=50) were sensitive to 1 µg oxacillin, while 36.7% (n=29) showed resistance to this antibiotic.…”

Section: Resultsmentioning

confidence: 98%

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2023

J. Med. Chem. Sci.

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Staphylococcus aureus is a Gram-positive pathogen that is commonly associated with bovine mastitis, which is an infectious condition affecting the udder of cows. The objective of the present study is to evaluate the antimicrobial efficacy of methicillin, amoxicillin, and ampicillin for detecting methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from cases of bovine mastitis. Methodologically, it was established to carry out the antimicrobial activity using the plate disc diffusion method (Kirby-Bauer) with the use of amoxicillin and ampicillin at 2 µg and 10 µg, also following the recommendations of the Clinical and Laboratory Standards Institute. To detect resistant methicillin strains, oxacillin was used at 1 µg, and cefoxitin at 30 µg. The study was conducted against 50 strains of the bacteria under investigation, which were isolated and identified. The applied treatments demonstrated a highly significant effect (p<0.05). In addition, a resistance of 50% and 60% to concentrations of 2 µg of amoxicillin and ampicillin, respectively, was observed. Furthermore, a resistance of 60% and 68% to 10µg of amoxicillin and ampicillin, respectively, was observed, indicating that Staphylococcus aureus is developing mechanisms that confer antimicrobial resistance. To further investigate this, the antimicrobial activity was conducted using 1 µg oxacillin discs and 30 µg cefoxitin, revealing that 36% and 32% of the isolates were resistant to these drugs, respectively. Phenotypically, 32% (n=16) of the isolates were identified as methicillinresistant Staphylococcus aureus (MRSA), demonstrating resistance to all betalactams tested.

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Section: Resultsmentioning

confidence: 98%

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2023

J. Med. Chem. Sci.

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Staphylococcus Aureus Resistentes a Meticilina E Meropenem Em Leite De Vacas Com Mastite Subclínica/ Methicillin and Meropenem Resistant Staphylococcus Aureus in Milk of Cows With Subclinical Mastitis

Cited by 1 publication

References 24 publications

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