Staphylococcus aureus Toxins: Promoter or Handicap during Infection?

Löffler, Bettina; Tuchscherr, Lorena

doi:10.3390/toxins13040287

Cited by 5 publications

(1 citation statement)

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“…The formative nature of Staphylococcus aureus and the opportunistic pathogens that it causes to livestock and humans have made it the focus of researchers' attention because of the threats it poses to life on Earth through the complex mechanism that it uses in the secretion of nanoparticles that make it resistant to all antibiotics and immunity (1,2). Staphylococcus aureus is a Gram-positive bacteria whose behavior differs from the rest of the bacteria in the mechanism of avoiding the host's defense systems by protecting the host's ketonemic antimicrobials, which helps them build and form biofilms on the vital materials of the infected organism to ensure its survival (3). SAK is one of the enzymes produced by Staphylococcus aureus bacteria, as this enzyme is produced by more than 100 strains of these bacteria.…”

Section: Introductionmentioning

confidence: 99%

Cloning & expression and production SAK enzyme from staphylococcus aureus and transfer in bacteria E. coli

Mutar

Panchal

2022

ijhs

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Staphylococcus aureus is one of the most dangerous organisms that threatens the life of humans and animals alike. It has been found that people who suffer from chronic diseases such as diabetes, blood vessels, pulmonary and cancerous diseases are more susceptible to infection, in addition to ability resist antibiotics, which made fierce and dangerous. This staphylococcus aureus possessed the SAK gene, which acts as a bio stimulant that plays an important role in the process of analyzing blood coagulation, as it acts as an anti-plasmogen activator and is in the form of an equal compound of human plasmin. SAK consists of a chain of amino acids numbering 136 amino acids. The study aimed to isolate the SAK gene using amplification technique PCR and insert it into an ideal vectorPET21a. And he transferred it to one of the strains of the E.coli(BL21DE3) , stimulating the bacteria by adding IPTG, the purpose of obtaining the highest level of protein expression in an ideal time, then purifying the protein by affinity chromatography.

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Section: Introductionmentioning

confidence: 99%