Staphylococcus xylosus VITURAJ10: Pyrrolo [1,2α] pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl) (PPDHMP) producing, potential probiotic strain with antibacterial and anticancer activity

Mangrolia, Upasana; Osborne, W. Jabez

doi:10.1016/j.micpath.2020.104259

Cited by 30 publications

(14 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MUM 256 [11,12,46]. The compound pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2methylpropyl) exhibits a wide range of biological activities such as antioxidant, antimicrobial and anticancer activities [47][48][49][50]. Benzeneacetamide was also produced by S. omiyaensis SCH2 and S. pluripotens MUSC137 from mangrove sediments [18,44].…”

Section: Discussionmentioning

confidence: 99%

Antibacterial, antioxidant, cytotoxic effects and GC-MS analysis of mangrove-derived Streptomyces achromogenes TCH4 extract

Tangjitjaroenkun

Pluempanupat

Tangchitcharoenkhul

et al. 2021

Arch biol sci (Beogr)

View full text Add to dashboard Cite

The in vitro biological activities of the ethyl acetate extract of the culture filtrate from Streptomyces achromogenes TCH4 (TCH4 extract) were evaluated. The ethyl acetate extract of TCH4 produced a bacteriostatic effect against Enterobacter cloacae, Staphylococcus aureus, Staphylococcus saprophyticus, Bacillus subtilis, methicillin-resistant S. aureus (MRSA) and Klebsiella pneumoniae. The extract had bactericidal activity against S. aureus, S. saprophyticus, S. aureus (MRSA) and K. pneumoniae with minimum bactericidal concentration (MBC) values in the range of 500-1000 ?g/mL. The total phenolic and flavonoid contents in TCH4 were 107.20?2.57 mg gallic acid equivalent (GAE)/g and 44.91?0.84 mg quercetin equivalent (QE)/g of dry extract. Antioxidant activity was determined by DPPH radical (IC50 299.64?6.83 ?g/mL) and ABTS radical scavenging (IC50 65.53?0.95 ?g/mL), and the ferric-reducing antioxidant power (FRAP) (822.76?9.12 mM FeSO4.7H2O/g dry extract) assays. TCH4 exhibited cytotoxic activity in the DU-145 cell line (IC50 9.36?0.37 ?g/mL). Analysis of extract constituents by GC-MS revealed pyrrolo[1,2-a]pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl) (36.85%), benzeneacetamide (23.76%), and deferoxamine (12.85%) as the major compounds, which have been reported to possess pharmaceutical properties. S. achromogenes TCH4 could be a potential source of bioactive metabolites with antibacterial, antioxidant and anticancer activities for pharmaceutical applications.

show abstract

Section: Discussionmentioning

confidence: 99%

Antibacterial, antioxidant, cytotoxic effects and GC-MS analysis of mangrove-derived Streptomyces achromogenes TCH4 extract

Tangjitjaroenkun

Pluempanupat

Tangchitcharoenkhul

et al. 2021

Arch biol sci (Beogr)

View full text Add to dashboard Cite

show abstract

“…Mangrolia and Osborne analyzed the antimicrobial and anticancer potential of the bacterial metabolites extracted from probiotic bacterial strains isolated from goat milk. [11] One of the isolates identified as Staphylococcus xylosus showed growth suppressing activity towards pathogenic strains such as Escherichia coli, Salmonella enterica and Staphylococcus aureus and was selected for further analysis. The bacterial metabolites extracted with chloroform showed maximum activity, thus the metabolites in chloroform extract were fractionated using a solid-phase extraction (SPE) column and the individual fractions tested against pathogens.…”

Section: Pseudomonas Sp (Nd)mentioning

confidence: 99%

“…Production of other types of nitrogen-compounds, such as amides, pyrazines, pyridines and nitriles, has also been reported. [7][8][9][10][11][12] Nitrogen containing heterocyclic compounds (pyridine, indole, pyrrole, etc.) are the base molecules in several biologically active natural products available as traditional medications or approved prescribed drugs.…”

Section: Introductionmentioning

confidence: 99%

Volatile Nitrogenous Compounds from Bacteria: Source of Novel Bioactive Compounds

Valença

Barbosa

Souto

et al. 2021

Chemistry & Biodiversity

View full text Add to dashboard Cite

Bacteria can produce nitrogenous compounds via both primary and secondary metabolic processes. Many bacterial volatile nitrogenous compounds produced during the secondary metabolism have been identified and reported for their antioxidant, antibacterial, antifungal, algicidal and antitumor activities. The production of these nitrogenous compounds depends on several factors, including the composition of culture media, growth conditions, and even the organic solvent used for their extraction, thus requiring their identification in specific conditions. In this review, we describe the volatile nitrogenous compounds produced by bacteria especially focusing on their antimicrobial activity. We concentrate on azo-compounds mainly pyrazines and pyrrolopyridines reported for their activity against several microorganisms. Whenever significant, extraction and identification methods of these compounds are also mentioned and discussed. To the best of our knowledge, this is first review describing volatile nitrogenous compounds from bacteria focusing on their biological activity.

show abstract

“…The different chloropyrazine-tethered pyrimidine derivatives (22)(23)(24)(25)(26)(27)(28)(29)(30)(31)(32)(33)(34)(35)(36)(37)(38)(39)(40) were obtained by the condensation of chloropyrazinyl chalcones (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19) with guanidine hydrochloride (Scheme 1) using a base catalyst (KOH) in yields ranging from 56-92%. The molecular structures of the chloropyrazine-tethered pyrimidine hybrids were disentangled using FT-IR and 1 H NMR spectral data and are presented in our previous paper [55].…”

Section: Chemistrymentioning

confidence: 99%

“…Examples of drugs with a pyrimidine nucleus are antibacterial and antimalarial agents targeting the folate pathway: trimethoprim, iclaprim, sulfadimethoxine, pyrimethamine, and sulfadoxine; antimetabolite and ergosterol biosynthesis inhibitor antifungal drugs: flucytosine and voriconazole; antimetabolite anti-cancer agents: 5-fluorouracil and capecitabine; tyrosine kinase inhibitors: imatinib, dasatinib, and nilotinib; antivirals: ara-c, idoxuridine, zidovudine, stavudine, lamivudine, and emtricitabine; and the antitubercular drug capreomycin (Figure 1). Many substituted pyrazine derivatives were reported with potential antibacterial [6][7][8][9][10][11], antifungal [12][13][14][15], anticancer [16][17][18][19][20][21], antioxidant [22][23][24][25], and antitubercular [9,[26][27][28] activities. Similarly, pyrimidine-based compounds also showed excellent antimicrobial (antibacterial and antifungal) [29][30][31][32][33], antimycobacterial [34][35][36][37][38], antioxidant [39][40][41][42], cytotoxic and anticancer [42][43][44]…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Antimicrobial and Antiproliferative Activities of Chloropyrazine-Tethered Pyrimidine Derivatives: In Vitro, Molecular Docking, and In-Silico Drug-Likeness Studies

Bhandare

Shaik

2021

Applied Sciences

View full text Add to dashboard Cite

Molecular hybridization (MH) of heterocyclic rings has enabled scientists to design and develop novel drugs and drug-like candidates. In our previous work, considering the importance of MH, we synthesized different kinds of chloropyrazine-tethered pyrimidine derivatives (22–40) containing either substituted phenyl or heteroaryl rings at position-6 of the pyrimidine ring and evaluated their antitubercular activity. Herein, we report the antimicrobial and antiproliferative activities of 22–40. The antiproliferative activity of the target hybrids was superior to the antimicrobial activity. However, some compounds showed greater antimicrobial activity than the standard drugs. For instance, among the nineteen derivatives, compound 31 containing a 2″,4″-dichlorophenyl ring, showed the most potent antibacterial and antifungal activities (MIC 45.37 µM), followed by compounds 25 and 30 bearing 4″-nitrophenyl and 2″,4″-difluorophenyl scaffolds with minimum inhibitory concentrations (MIC) values of 48.67 µM and 50.04 µM, respectively. Compound 35, containing a bioisosteric 2″-pyridinyl ring, showed the most potent antiproliferative activity against the prostate cancer cell line (DU-145) with an IC50 value of 5 ± 1 µg/mL. Additional testing of compounds 22–40 on human normal liver cells (LO2) indicated that the compounds were more selective to cancer cell lines over normal cells. Further, molecular docking of the most potent compound 35 against dihydrofolate reductase (DHFR) (PDB ID: 1U72) had a good binding affinity with a docking score of −6.834. The SwissADME program estimated the drug-likeness properties of compound 35. Hybrid 35 is a potential lead molecule for the development of new anticancer drugs, whereas 31 is a promising antimicrobial lead candidate.

show abstract

Staphylococcus xylosus VITURAJ10: Pyrrolo [1,2α] pyrazine-1,4-dione, hexahydro-3-(2-methylpropyl) (PPDHMP) producing, potential probiotic strain with antibacterial and anticancer activity

Cited by 30 publications

References 35 publications

Antibacterial, antioxidant, cytotoxic effects and GC-MS analysis of mangrove-derived Streptomyces achromogenes TCH4 extract

Antibacterial, antioxidant, cytotoxic effects and GC-MS analysis of mangrove-derived Streptomyces achromogenes TCH4 extract

Volatile Nitrogenous Compounds from Bacteria: Source of Novel Bioactive Compounds

Assessment of the Antimicrobial and Antiproliferative Activities of Chloropyrazine-Tethered Pyrimidine Derivatives: In Vitro, Molecular Docking, and In-Silico Drug-Likeness Studies

Contact Info

Product

Resources

About