Starch and lipid accumulation in eight strains of six Chlorella species under comparatively high light intensity and aeration culture conditions

Takeshita, Tsuyoshi; Ota, Shuhei; Yamazaki, T.; Hirata, Aiko; Zachleder, Vilém; Kawano, Shigeyuki

doi:10.1016/j.biortech.2014.01.135

Cited by 125 publications

(74 citation statements)

References 34 publications

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“…The Chlorophyta, among 11 oleaginous species, showed the greatest increase in lipid content (almost doubled or even more) in response to nitrogen limitation (Griffiths et al 2011). Besides lipids, starch content also can be promoted under nitrogen deficiency in green algae (Dragone et al 2011;Li et al 2011b;Yao et al 2013;Takeshita et al 2014). Upon nitrogen starvation, both starch and lipid contents increased greatly within cells, but starch synthesis precedes lipid accumulation in Chlorella zofingiensis and, after 2 days of stress condition, starch was possibly degraded to support lipid synthesis (Zhu et al 2014).…”

Section: Introductionmentioning

confidence: 99%

High temperature enhances lipid accumulation in nitrogen-deprived Scenedesmus obtusus XJ-15

Xia

Song

2015

J Appl Phycol

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This study investigated the changes in lipid and starch contents, lipid fraction, and lipid profile in the nitrogen-starved Scenedesmus obtusus XJ-15 at different temperatures (17, 25, and 33°C). The optimal temperature for both growth and lipid accumulation under nitrogen-sufficient condition was found to be 25°C. However, under nitrogen deprivation, the total and neutral lipids increased with increasing temperature, and achieved the highest lipid content of 47.60 % of dry cell weight and the highest TAG content of 79.66 % of total lipid at 33°C. In the meantime, the stored cellular starch content decreased with the increasing temperature. Thus, high temperature induced carbon flux from starch toward TAG accumulation in microalgae during nitrogen starvation. In addition, the decreased polar lipids may also serve for TAG synthesis under high temperature, and high temperature further reduced the degree of the fatty acid unsaturation and favored a better biodiesel production. These results suggested that high-temperature stress can be a good strategy for enhancing biofuel production in oleaginous microalgae during nitrogen deficiency.

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Section: Introductionmentioning

confidence: 99%

High temperature enhances lipid accumulation in nitrogen-deprived Scenedesmus obtusus XJ-15

Xia

Song

2015

J Appl Phycol

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“…Quality and quantity of biomass and oil yield of algae also depends on the kind of species and strains from local environments [15]. During in vitro culture of algae, the factors that influence productivity include media composition and cultural conditions [16]. Factors in the medium of growth have direct influence on specific product accumulations in algae; especially algal fatty acid composition within the biomass [17].…”

Section: Introductionmentioning

confidence: 99%

Biomass Productivity and Fatty Acid Composition of Chlorella lobophora V M Andreyeva, a Potential Feed Stock for Biodiesel Production

Kookal¹,

Santhakumaran²,

Ray³

2015

AJPS

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Green algae represent the fastest producible source of biomass that has diverse uses as biofuel, food, feed-stocks and the like. The biomass is rich in oils, which can be used as biofuel and the cultivation may be used to purify water as well. Productivity and quality of algal biomass depends on species and strains as well as environmental conditions of growth. Therefore, identification of new species and strains as well as standardization of media composition and environmental conditions for optimum yield of specific products, especially oil has a high relevance in algal technological research. In this connection, biomass productivity and oil yield of a local strain of Chlorella lobophora in BBM and modified BBM media are compared. Chemical characterization of the oil and feasibility of biodiesel production from the extracted oil is also assessed. Biomass productivity and the lipid productivity of the alga in both the media are found to be significantly different (P < 0.05). Fatty acid profiling of the oil extracted from the dried algal biomass using GC-MS analysis reveals that Palmitic acid (16:0) and Oleic acid (18:1) are the major fatty acids and nutraceutically important fatty acids are also present. The oil can be effectively transesterified to methyl esters. FTIR spectroscopy confirms the quality of the biodiesel produced. As the potentials of this local algal strain for biomass production and oil yield is confirmed, further standardization of environment conditions for better oil yield and quality biomass production is progressing to optimize its value as globally competent nutraceutical and biofuel resource.

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“…After mincing, lipids components were extracted with methyl tertiary-butyl ether and analyzed with the gas chromatograph mass spectrometry (GC-MS; QP-2010Plus, Shimadzu, Kyoto) equipped with Supelco SP-2380 column (30 m×0.25 mm×0.2 µm) (Sigma-Aldrich). These preparation and measurement are according to Takeshita et al (Takeshita et al 2014).…”

Section: Methodsmentioning

confidence: 99%

Naturally grown rucola, <i>Eruca sativa</i>, contains more α-linolenic acid than conventionally grown rucola

Ohta

Takeshita

Funabashi

et al. 2016

Plant Biotechnology

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The ratio of dietary n-6/n-3 polyunsaturated fatty acids is higher in the modern Western meal when compared with that of the Paleolithic era. Evidences have been accumulating that the extremely high ratio of dietary n-6/n-3 polyunsaturated fatty acids increases the risk of disease and the deterioration of physical conditions among humans. In this study, the ratio of linoleic acid (C18:2, n-6) and α-linolenic acid (C18:3, n-3) of rucola, Eruca sativa, was compared between naturally grown samples and conventional farming products (n=3 for each). We found that the naturally grown rucola contained significantly higher amount of α-linolenic acid (p=0.026), resulting in the lower ratio of linoleic acid and α-linolenic acid than the conventional (p=0.016). This finding suggests that vegetables cultured in conventional farming method could decrease the health promotion effects that the vegetables originally possess in natural environment.

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Starch and lipid accumulation in eight strains of six Chlorella species under comparatively high light intensity and aeration culture conditions

Cited by 125 publications

References 34 publications

High temperature enhances lipid accumulation in nitrogen-deprived Scenedesmus obtusus XJ-15

High temperature enhances lipid accumulation in nitrogen-deprived Scenedesmus obtusus XJ-15

Biomass Productivity and Fatty Acid Composition of Chlorella lobophora V M Andreyeva, a Potential Feed Stock for Biodiesel Production

Naturally grown rucola, <i>Eruca sativa</i>, contains more α-linolenic acid than conventionally grown rucola

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