Starvation‐induced degradation of yeast hexose transporter Hxt7p is dependent on endocytosis, autophagy and the terminal sequences of the permease

Krampe, Stefanie; Boles, Eckhard

doi:10.1016/s0014-5793(02)02297-4

Cited by 42 publications

(51 citation statements)

References 30 publications

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“…Such a mechanism has been described for several yeast sugar transporters, including Hxt5p (van Suylekom et al 2007). In addition, GFP fusions of Hxt2p, Hxt6p, and Hxt7p were degraded in vacuoles by a similar mechanism described in degradation of Hxt5p (Krampe and Boles 2002;Kruckeberg et al 1999).…”

Section: Discussionmentioning

confidence: 79%

Functional Characterization of fst1 in Fusarium verticillioides During Colonization of Maize Kernels

Kim¹,

Woloshuk²

2011

MPMI

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The putative hexose transporter gene fst1 in Fusarium verticillioides was identified previously by microarray analysis as a gene that was more highly expressed during colonization of autoclaved maize endosperm than germ. In contrast to a previous study, in which disruption of fst1 did not affect growth of the pathogen on autoclaved maize kernels, in the current study, we demonstrated that disruption of fst1 delayed growth and symptom development on wounded maize ears. Characterization of the fst1 promoter revealed that regulation of fst1 expression was similar to that of fumonisin biosynthetic (fum) genes; expression was highest during growth on endosperm tissue and repressed by elevated concentrations of ammonium in the growth medium. With a fluorescent tag attached to FST1, the protein localized transiently to the periphery of the cells near the plasma membrane and in vacuole-like structures, suggesting that membrane-localized FST1 was internalized and degraded in vacuoles. Expression of fst1 in a yeast strain lacking hexose transporter genes did not complement the yeast mutation, suggesting that FST1 does not transport glucose, fructose, or mannose. The results indicate a functional role for FST1 in pathogenesis during the colonization of living kernels.

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Section: Discussionmentioning

confidence: 79%

Functional Characterization of fst1 in Fusarium verticillioides During Colonization of Maize Kernels

Kim¹,

Woloshuk²

2011

MPMI

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“…Therefore, it is feasible that Hxts are phosphorylated following treatment with MG prior to endocytosis. Hxt7 lacking the cytoplasmic domain at the N terminus was hardly internalized and degraded under conditions where the endocytosis of Hxt7 was induced (high glucose conditions) (51). It has been reported that the PEST (Pro-Glu-Ser-Thr) sequence found at the N terminus of Hxt7 is involved in the degradation (52,53).…”

Section: Discussionmentioning

confidence: 99%

Reduction of Glucose Uptake through Inhibition of Hexose Transporters and Enhancement of Their Endocytosis by Methylglyoxal in Saccharomyces cerevisiae

Yoshida

Wei

Nomura

et al. 2012

Journal of Biological Chemistry

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Background: Methylglyoxal is a typical 2-oxoaldehyde derived from glycolysis. Results: Methylglyoxal inhibited the activity of yeast hexose transporters as well as mammalian glucose transporters (GLUT1 and GLUT4) and enhanced the endocytosis of hexose transporters in yeast. Conclusion: Methylglyoxal inhibited glucose uptake in yeast cells. Significance: Phospholipase C and protein kinase C were involved in endocytosis of hexose transporters.

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“…The Hxtp hexose transporters that play a key role in the control of sugar flux in fermentation are controlled in this way (Krampe and Boles 2002). This mechanism, in combination with transcriptional control, modulates the abundance of Hxtp carriers in the plasma membrane (Perez et al 2005).…”

Section: Effect Of Starvation On Protein Fate and Amino Acid Homeostasismentioning

confidence: 99%

Responses of Saccharomyces cerevisiae to nitrogen starvation in wine alcoholic fermentation

Tesnière

Brice

Blondin

2015

Appl Microbiol Biotechnol

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Nitrogen is an important nutrient in alcoholic fermentation because its starvation affects both fermentation kinetics and the formation of yeast metabolites. In most alcoholic fermentations, yeasts have to ferment in nitrogen-starved conditions, which requires modifications of cell functions to maintain a high sugar flux and enable cell survival for long periods in stressful conditions. In this review, we present an overview of our current understanding of the responses of the wine yeast Saccharomyces cerevisiae to variations of nitrogen availability. Adaptation to nitrogen starvation involves changes in the activity of signaling pathways such as target of rapamycin (TOR) and nitrogen catabolite repression (NCR), which are important for the remodeling of gene expression and the establishment of stress responses. Upon starvation, protein degradation pathways involving autophagy and the proteasome play a major role in nitrogen recycling and the adjustment of cellular activity. Recent progress in the understanding of the role of these mechanisms should enable advances in fermentation management and the design of novel targets for the selection or improvement of yeast strains.

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Starvation‐induced degradation of yeast hexose transporter Hxt7p is dependent on endocytosis, autophagy and the terminal sequences of the permease

Cited by 42 publications

References 30 publications

Functional Characterization of fst1 in Fusarium verticillioides During Colonization of Maize Kernels

Functional Characterization of fst1 in Fusarium verticillioides During Colonization of Maize Kernels

Reduction of Glucose Uptake through Inhibition of Hexose Transporters and Enhancement of Their Endocytosis by Methylglyoxal in Saccharomyces cerevisiae

Responses of Saccharomyces cerevisiae to nitrogen starvation in wine alcoholic fermentation

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