Starvation Induces Endoplasmic Reticulum Stress and Autophagy in Malpighian Tubules of Two-Spotted Field Crickets Gryllus bimaculatus

Lee, Nuri; Song, Jaeki; Ko, Younghwa; Kwon, Kisang; Lee, Eun-Ryeong; Kwon, O‐Yu

doi:10.2317/0022-8567-93.1.88

Cited by 2 publications

(2 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Starvation stress is accompanied by a large number of complex physiological processes [ 22 , 23 ]. Transcriptomics can be used to study the overall structure and function of genes and can reveal the molecular mechanisms of biological processes; proteomics can be used to study the overall changes in protein expression during this process.…”

Section: Discussionmentioning

confidence: 99%

“…The Spearman's correlation coefficient is used to measure the monotonic association between two variables and is not limited to continuous random variables. The Kendall's correlation coefficient tests for correlation between two variables from the perspective of whether the two variables are consistent [21].…”

Section: Identification Of Salivary Gland Transcriptome and Proteomementioning

confidence: 99%

See 1 more Smart Citation

Starvation-induced changes in the proteome and transcriptome of the salivary glands of leech (Hirudo nipponia)

Cai,

Shen,

Xing

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

Hirudo nipponia is an important medicinal animal in China. Its salivary gland secretions contain a variety of protein bioactive substances. Investigations of its salivary glands are of great significance in the study of the medicinal value and mechanism of leech secretions. Illumina RNA-Seq technology was used to perform transcriptome sequencing of salivary gland tissue of H. nipponia under starvation (D30) and fed (D0) states. A total of 2,650 differentially expressed genes (DEGs) were screened. Using the label-free protein quantification technique and bioinformatics analysis, the expression of differentially expressed proteins (DEPs) in the salivary gland tissue of H. nipponia was compared. A total of 2,021 proteins were identified, among which 181 proteins were differentially expressed between the starvation and fed states, with 72 significantly upregulated and 109 significantly downregulated. The salivary glands of H. nipponia synthesized protein-based active substances after 30 days of starvation and adapted to the starvation environment by weakening respiratory activity and reducing metabolic activity to reduce energy expenditure. Energy was produced by glycolysis and the tricarboxylic acid cycle for the synthesis of substances such as antibiotics. This study combined transcriptome and proteome sequencing data to provide a data reference for an in-depth study of the regulatory mechanism of salivary gland secretions of H. nipponia under starvation stress by analyzing DEGs and DEPs.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Identification Of Salivary Gland Transcriptome and Proteomementioning

confidence: 99%

Starvation-induced changes in the proteome and transcriptome of the salivary glands of leech (Hirudo nipponia)

Cai,

Shen,

Xing

et al. 2024

PLoS ONE

View full text Add to dashboard Cite

show abstract

Identification and Expression Analysis of a cDNA Encoding Cyclophilin A from Gryllus bimaculatus (Orthoptera: Gryllidae)

Kwon

Lee

Kang

et al. 2023

Wseas Transactions on Environment and Development

View full text Add to dashboard Cite

Cyclophilin A (CypA), a cytosolic binding protein of cyclosporine A, is an immunosuppressive drug. In this study, CypA cDNA was cloned from the two-spotted cricket Gryllus bimaculatus (gCypA). The protein encoded by gCypA comprises 165 amino acids with a molecular mass of 19.23 kDa and an isoelectric point of 9.38 and possesses three N-glycosylation sites and 17 phosphorylation sites. The secondary and tertiary structures of gCypA were identified, and homology analysis revealed that it shares around 73%-81% sequence identities with other CypA proteins. When the researchers analyzed the expression levels of gCypA mRNA in various tissues, they found that the foregut exhibited nearly the same expression level as that of the dorsal longitudinal flight muscle (the control). However, gCypA mRNA expression in the fat body, Malpighian tubes, and midgut was less than half of that in the dorsal longitudinal flight muscle. Under endoplasmic reticulum stress conditions, gCypA mRNA expression was highest in Malpighian tubules (about two times higher than the expression in the control). Under starvation conditions, gCypA mRNA expression increased to three times that of the dorsal longitudinal flight muscle 6 days after starvation. Nonetheless, its expression levels decreased in Malpighian tubules under all starvation conditions. This study provides insights into the physiological role of gCypA in G. bimaculatus.

show abstract

Starvation Induces Endoplasmic Reticulum Stress and Autophagy in Malpighian Tubules of Two-Spotted Field Crickets Gryllus bimaculatus

Cited by 2 publications

References 38 publications

Starvation-induced changes in the proteome and transcriptome of the salivary glands of leech (Hirudo nipponia)

Starvation-induced changes in the proteome and transcriptome of the salivary glands of leech (Hirudo nipponia)

Identification and Expression Analysis of a cDNA Encoding Cyclophilin A from Gryllus bimaculatus (Orthoptera: Gryllidae)

Contact Info

Product

Resources

About