STAT3 induces colorectal carcinoma progression through a novel miR-572-MOAP-1 pathway

Abstract

PurposeColorectal carcinoma (CRC) is among the most common causes of death. Recent studies have shown that both STAT3 and miR-572 contribute to CRC progression. STAT3 plays an important role in miRNA expression. Moreover, MOAP-1, which is a pro-apoptotic protein that induces cell death or apoptosis, has a direct correlation with miRNA. Therefore, the current study is designed to explore whether miR-572 and STAT3 are involved in a common pathway and the role of MOAP-1 in this process.Patients and methodsThe expressions of STAT3, miR-572, and MOAP-1 in human CRC tissues and multiple cell lines were estimated by qRT-PCR or Western blot. MTT, transwell migration, and invasion assays were used to assess cell growth, migration, and invasion, respectively. Dual-luciferase reporter assay was applied to examine the association between miR-572 and MOAP-1.ResultsElevated STAT3 levels were accompanied by increased miR-572 and decreased MOAP-1 levels in primary CRC specimens and cell lines. STAT3 promoted CRC cell growth, migration, and invasion via the upregulated expression of miR-572. Subsequently, miR-572 inhibited MOAP-1 protein expression through an interaction with its 3′UTR.ConclusionOur study proposes a novel STAT3-miR-572-MOAP-1 pathway involved in the process of CRC progression, which might be a potential target for the development of new preventive and therapeutic approaches against human colorectal cancer.

Role of epigenetics in transformation of inflammation into colorectal cancer

“…In addition, by activating miR-21, NR2F2 inhibits Smad7 expression and promotes TGF-β-dependent epithelial-mesenchymal transition (EMT) in CRC[88]. In primary CRC samples and cell lines, increased STAT3 expression levels are accompanied by elevated miR-572 and decreased MOAP-1 levels, and miR-572 has been found to reduce the expression of the proapoptotic protein MOAP-1 and to contribute to CRC progression[89]. Öner et al[90] demonstrated that combined inactivation of TP53 and miR-34a promotes CRC metastasis by elevating the levels of IL-6R and PAI1, implying that modifying these processes might be alternative approaches to treat CRC.…”

The role of microRNA-572 in the proliferation and chemotherapeutic treatment of prostate cancer

“…Studies have shown that aberrant expression of miRNAs involves different signaling pathways in various cancers, including PCa cancer. Wang et al 23 reported that elevated signal transducer and activator of transcription 3 (STAT3) expression increased proliferation, migration, and invasion of colorectal cancer cells via upregulation of miR-572. Moreover, increased miR-572 expression has been observed in renal cell carcinoma (RCC) tissues and cell lines.…”

Dose‑dependent expression of extracellular microRNAs in HCT116 colorectal cancer cells exposed to high‑dose‑rate ionising radiation

“…Within the data presented herein, it was observed that 8 of 12 miRNAs (miR-422a, miR-197, miR-1183, miR-1288, miR-1229, miR-423-5p, miR-939 and miR-572) have a function in CRC. Intracellular expression of miR-422a, miR-572 and miR-939 serve a role in tumour suppression; miR-422a targets AKT1 and MAPK1; miR-572 targets the MOP-1 pathway activated by STAT3 and miR-939 targets NF-κB (40)(41)(42)(43)(44). Of those, miR-572 and miR-939 were also among the three most highly expressed miRNAs, with the highest chance of detection in the blood.…”