State of antioxidant systems and phenolic compounds’ production in Hypericum perforatum L. hairy roots

Tusevski, Oliver; Станоева, Јасмина Петреска; Стефова, Марина; Spasenoski, Mirko; Simic, Sonja Gadzovska

doi:10.1007/s11738-019-2919-5

Cited by 19 publications

(60 citation statements)

References 66 publications

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“…It was shown by Franklin et al ( 2008 ) on the Hypericum perforatum L. model that the most limiting factor in successful genetic transformation of plants using Rhizobium bacteria is plant’s recalcitrance. When plant cells are challenged with Rhizobium, the stress-induced defense response appears, based on induction of stress-involved gene expression patterns and up-regulation of enzymatic protein activity in the phenylpropanoid pathway, leading to increased synthesis of secondary metabolites (Franklin et al 2009 ; Tusevski et al 2019 ). Being a rich source of phenolic compounds with strong bactericidal properties, carnivorous plants from the family Droseraceae are hard to transform (Blehova et al 2015 ), while transformation of such organisms gives the possibility to study pathways involved in the synthesis of valuable secondary metabolites and/or overproducing phytochemicals of interest (Gandhi et al 2015 ).…”

Section: Discussionmentioning

confidence: 99%

“…Rol genes (the root loci) are essential for hairy root creation, while the phenotype of transformed plants depends on the number of rol genes incorporated in plant DNA and their expression pattern (Vinterhalter et al 2015 ). High expression of the rol gene family particularly stimulates hairy root creation and elongation (Tusevski et al 2019 ). The transformation of D. muscipula did not affect hairy root formation.…”

Section: Discussionmentioning

confidence: 99%

“…Since fast biomass accumulation in plant tissue cultures is one of the most important features in the industry field, research has been conducted to determine the growth parameters of hairy root cultures obtained after R. rhizogenes inoculation (Georgiev et al 2007 ). Tusevski et al ( 2019 ) showed great variation in the growth of H. perforatum hairy roots, although each clone was obtained using the same bacterial strain. Similar variability of growth parameters was observed by Tusevski et al ( 2017 ).…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, little is known about the metabolism of secondary compounds after the incorporation of bacterial T-DNA in the plant genome. Tusevski et al ( 2019 ) reported that hairy root culture had the same, or an even greater, ability to produce valuable metabolites. It is postulated that the rol genes family can act as an endogenous elicitor and conduct changes in the phytochemical profile of medicinal plants (Tusevski et al 2019 ).…”

Section: Discussionmentioning

confidence: 99%

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Transformed tissue of Dionaea muscipula J. Ellis as a source of biologically active phenolic compounds with bactericidal properties

Makowski

Królicka

Nowicka

et al. 2021

Appl Microbiol Biotechnol

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The Venus flytrap (Dionaea muscipula J. Ellis) is a carnivorous plant able to synthesize large amounts of phenolic compounds, such as phenylpropanoids, flavonoids, phenolic acids, and 1,4-naphtoquinones. In this study, the first genetic transformation of D. muscipula tissues is presented. Two wild-type Rhizobium rhizogenes strains (LBA 9402 and ATCC 15834) were suitable vector organisms in the transformation process. Transformation led to the formation of teratoma (transformed shoot) cultures with the bacterial rolB gene incorporated into the plant genome in a single copy. Using high-pressure liquid chromatography, we demonstrated that transgenic plants were characterized by an increased quantity of phenolic compounds, including 1,4-naphtoquinone derivative, plumbagin (up to 106.63 mg × g−1 DW), and phenolic acids (including salicylic, caffeic, and ellagic acid), in comparison to non-transformed plants. Moreover, Rhizobium-mediated transformation highly increased the bactericidal properties of teratoma-derived extracts. The antibacterial properties of transformed plants were increased up to 33% against Staphylococcus aureus, Enterococcus faecalis, and Escherichia coli and up to 7% against Pseudomonas aeruginosa. For the first time, we prove the possibility of D. muscipula transformation. Moreover, we propose that transformation may be a valuable tool for enhancing secondary metabolite production in D. muscipula tissue and to increase bactericidal properties against human antibiotic-resistant bacteria. Key points • Rhizobium-mediated transformation created Dionaea muscipula teratomas. • Transformed plants had highly increased synthesis of phenolic compounds. • The MBC value was connected with plumbagin and phenolic acid concentrations.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Transformed tissue of Dionaea muscipula J. Ellis as a source of biologically active phenolic compounds with bactericidal properties

Makowski

Królicka

Nowicka

et al. 2021

Appl Microbiol Biotechnol

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“…Moreover, when AHRs were cultured in the optimized procedure, it revealed an outstanding effect in comparison with the original procedure. Many previous studies have demonstrated that the integration of rol genes indirectly affected the expression of enzymes belonging to the biosynthesis pathway of secondary metabolites, leading to an increase in the bioactivity level [27,28]. Tusevski et al found that the integration of rolB into plant cells through the Agrobacterium rhizogenes transformation process activated the phenylalanine ammonia lyase activity of H. perforatum, leading to increased flavonoid production [29].…”

Section: Optimization Of Exogenous Factors For the Ahrs And Rol Gene mentioning

confidence: 99%

Developing a Sufficient Protocol for the Enhancement of α-Glucosidase Inhibitory Activity by Urena lobata L. Aeroponic Hairy Roots Using Exogenous Factors, a Precursor, and an Elicitor

Cao

Hoang

et al. 2020

Plants

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Aeroponics is considered as a potential method for the culture of herbal plants due to the high growth rate, quantity and quality enhancement of secondary metabolites, and substantial environmental progress associated with this method. The aim of this study was to develop a sufficient protocol for successful Urena lobata hairy root induction by Agrobacterium rhizogenes ATCC 15834, using a precursor and elicitor to enhance α-glucosidase inhibitory activity (GIA) of aeroponic hairy roots (AHRs) in greenhouse conditions. In this study, we found that the optimized procedure (10 min, Woody plant medium (WPM), 1/25 salt strength) had an outstanding effect with a reduction in the rooting time (RT), promotion of the rooting rate (RR), and increase in the fresh weight (FW) and dry weight (DW) compared with the original procedure (30 min, Murashige and Skoog (MS) medium, 1/25 salt strength) after 30 days of culture. The highest DW, GIA, flavonoid (FLA) and phenolic (PHEL) contents were observed for individual addition of 10 mM phenylalanine (PA) or 50 mM chitosan (CS) in the late exponential phase (eighth week) with 15 days of elicitation compared to the control AHRs. However, individual treatment was less effective than the combination of the two. Positive correlations among the GIA, FLA and PHEL indicate that AHRs accumulated phenolic compounds, leading to an increase in the GIA by a synergistic effect. In conclusion, the culture of Urena lobata AHRs with PA and CS is an efficient procedure to produce GIA material in greenhouse conditions. The treatment of type II diabetes is sophisticated in terms of the variety of risk factors, one of which is the considerably rapid development of postprandial hyperglycemia [5]. Postprandial hyperglycemia occurs through the reaction of α-glucosidase and α-amylase in order to produce glucose. Therefore, by restricting or reducing the activity of these enzymes, the occurrence of postprandial hyperglycemia should decrease. The inhibition of α-glucosidase activity leads to a deterioration in disaccharide hydrolysis which has substantial long-term effects on glycemic index control in diabetic patients [6]. In recent years, natural medicines from plants have been used as useful treatments without any side effects.Hairy roots (HRs) are established from the interaction between Agrobacterium rhizogenes and the host plant (gene transformation from bacteria to the genome of the plant) [7]. There are numerous merits that accrue from the collaboration; in particular, a high growth rate without the dependence of any exogenous hormones, genetic stability, and a high branching rate. In addition, the ability to produce secondary metabolites remains stable. Hence, the HR cultural technique is considered to be an attractive substitute for the manufacture of tremendously valuable natural secondary metabolites instead of bud or callus cultures [8].Accompanied by the significant progress of modern techniques, aeroponics is a new method that has been adapted from classical hydroponics. Compared with hydropon...

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UPLC‐MS‐Based Metabolomics Profiling and Chemometric Analysis for Hypericum sinaicum Boiss and the Endophytic Aspergillus foetidus in Comparison to Hypericum perforatum L.

Sheashea,

El‐Hawary,

Ahmed

et al. 2023

Chemistry & Biodiversity

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One of the endangered plant species in Saint Catherine protectorate is Hypericum sinaicum Boiss which is endemic to Egypt, Jordan, and Saudi Arabia. The fungus‐host relationship can assist in the investigation of bioactive compounds produced by H. sinaicum paving the way for economic and medicinal implications. Therefore, a comprehensive metabolic approach via MS and chemical analysis was used to track and compare metabolites from H. sinaicum and Aspergillus foetidus var. pallidus, the endophytic fungus, with Hypericum perforatum. Metabolomics analysis revealed the presence of 25 metabolites distributed among samples and the discovery of new chemotaxonomic compounds, i.e., phloroglucinols and xanthones, allowing the discrimination between species. A. foetidus extract is considered a reliable source of furohyperforin and naphthodianthrone derivatives. In conclusion, using A. foetidus as an in vitro technique for producing potential phytoconstituents was cost effective, having easier optimization conditions and faster growth with fewer contamination rates than other in vitro methods.

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State of antioxidant systems and phenolic compounds’ production in Hypericum perforatum L. hairy roots

Cited by 19 publications

References 66 publications

Transformed tissue of Dionaea muscipula J. Ellis as a source of biologically active phenolic compounds with bactericidal properties

Transformed tissue of Dionaea muscipula J. Ellis as a source of biologically active phenolic compounds with bactericidal properties

Developing a Sufficient Protocol for the Enhancement of α-Glucosidase Inhibitory Activity by Urena lobata L. Aeroponic Hairy Roots Using Exogenous Factors, a Precursor, and an Elicitor

UPLC‐MS‐Based Metabolomics Profiling and Chemometric Analysis for Hypericum sinaicum Boiss and the Endophytic Aspergillus foetidus in Comparison to Hypericum perforatum L.

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