State of collagenolysis in experimental periodontitis of bacterial-immune genesis and its correction with flavonol

Demkovych, Andrii; Bondarenko, Yu. I.; Fastovets, O. O.; Hrad, A O; Hasiuk, P. А.

doi:10.26641/2307-0404.2021.2.234488

Cited by 15 publications

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“…It is known that bacteria are constantly present in the oral cavity, which are in direct contact as part of dental plaque with periodontal tissues and are capable of producing a large number of biologically active substances: lipolysaccharide toxin, leukotoxins, collagenases, hydrolytic proteases and others. According to the results of modern scientific research, it has been established that damage to periodontal tissue does not occur directly from the toxicity of bacterial factors, but from the ability of bacterial components to influence the immune response [10,11,12,13]. Therefore, it can be assumed that the development of periodontitis in patients of group 4 is a consequence of hyperactivity and dysregulation of the immune system in type 2 diabetes.…”

Section: Discussionmentioning

confidence: 99%

Features of Changes in Indicators of Local Immunity of the Oral Cavity and Blood Serum of Patients Against the Background of Generalized Periodontitis and Type 2 Diabetes Mellitus

Balitska,

Hasiuk,

Piasetska

et al. 2023

UESMS

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Aim: to evaluate the nature of changes in innate humoral immunity in oral fluid and blood serum in patients with generalized periodontitis against the background of type 2 diabetes mellitus and in their combination. Materials and methods. We examined 68 patients with a clinically established diagnosis of type 2 diabetes mellitus (of which 32 were additionally diagnosed with chronic generalized periodontitis), who were divided into 4 groups. The content of the main classes of immunoglobulins Ig A, Ig G and Ig M in blood serum and oral fluid was determined by radial immunodiffusion in a gel using monospecific antisera according to G. Manchini (Manchini G., 1965). Statistical processing of data was carried out using the Shapiro criterion. The level of statistical significance of differences between samples was assessed using the nonparametric Mann-Whitney test. Results. The lowest sIg A values and the highest Ig G values in the oral fluid were found in patients of group 4 with generalized periodontitis against the background of type 2 diabetes mellitus. A probable decrease in the level of sIg A was established, in particular, in the 2nd group by 1.27 times, the 3rd by 4.98 times and the 4th by 7.48 times in relation to the data of the control group (p<0, 01). The level of Ig G was probably higher in group 2 by 1.62 times, respectively, in group 3 – by 2.29 times and in group 4 – by 3.35 times, relative to control values (p<0.01 ). At the same time, when studying indicators in blood serum, the results of our studies indicate an imbalance of immunoglobulin fractions in the pathologies under study. Thus, the level of Ig A in the 2nd group was probably 1.7 times higher, in the 3rd group – 1.6 times higher, and in the 4th group – 2.0 times higher, relative to the data in the control group (p<0.001). The level of Ig M in the 2nd group was statistically significantly lower by 1.2 times, while in the 3rd and 4th groups it was probably higher by 1.7 and 1.2 times, respectively. The level of Ig G was probably higher in the 2nd and 3rd groups – by 1.3 times and in the 4th – by 2.0 times, relative to control values (p <0.05). This indicates that type 2 diabetes complicates the course of chronic periodontitis, as evidenced by changes in the concentrations of the main classes of immunoglobulins. Conclusions. Thus, generalized periodontitis against the background of type 2 diabetes mellitus is accompanied by significant changes in local immunity in biological fluids, as evidenced by disruption of the functioning of the humoral part of the adaptive immune system, which is manifested by an increase in the content of the main classes of immunoglobulins Ig A, Ig M, Ig A.

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Section: Discussionmentioning

confidence: 99%

Features of Changes in Indicators of Local Immunity of the Oral Cavity and Blood Serum of Patients Against the Background of Generalized Periodontitis and Type 2 Diabetes Mellitus

Balitska,

Hasiuk,

Piasetska

et al. 2023

UESMS

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“…Lipopolysaccharide inflammation of the periodontium in rats is accompanied by an increase in the level of endogenous intoxication, as indicated by an increase in the content of medium weight molecules [4]. Imbalance of thyroid hormones exacerbates endogenous intoxication in lipopolysaccharide periodontitis, especially in hyperthyroidism, which is further accompanied by connective tissue catabolism, as evidenced by a probable increase in collagenolytic activity, increased content of free oxyproline, growth of free oxyproline, growth increasing the content of markers of the breakdown of proteoglycans -glycosaminoglycans in the serum [15]. Thyroid dysfunction exacerbates the destruction of connective tissue in lipopolysaccharide inflammation of the periodontium in both hyperthyroidism and hypothyroidism.…”

Section: Discussionmentioning

confidence: 99%

Morphological Changes of Periodontal Components Under Experimental Lipopolysaccharide Periodontitis Combined With Hyperthyroidism

et al. 2022

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The aim: Investigate structural changes in the tissues of the periodontal complex under the condition of experimental lipopolysaccharide periodontitis combined with hyper¬thyroidism. Materials and methods: The studies were performed on adult white male rats, which simulated periodontitis combined with hyperthyroidism. Periodontal tissues were subjected to morphological examination on the 22nd day of the experiment. Collection of material for microscopic examinations was performed according to the generally accepted method; histological specimens were studied using a light optical microscope. Results: Morphological studies of the components of the periodontal complex of experimental animals with experimental periodontitis established the reorganization of its structural elements. Damage to the epithelium in the area of attachment of the circular ligament and erosive-ulcerative changes of the gums led to a deepening of the gingival sulcus with the formation of a deep periodontal pocket. Intense hyperkeratosis was observed in the area of the bottom of the periodontal pocket. In the own plate of the mucous membrane of the gums – significant edema, collagen fibers were disorganized, defragmented. There were pronounced destructive-degenerative and inflammatory changes of the epithelial and own plates of all areas of the gums and periodontium, damage to the nuclei and cytoplasm of keratinocytes, fibroblasts, and leukocytes. Conclusions: Experimental periodontitis combined with hyperthyroidism is accompanied by pronounced signs of destructive and inflammatory changes in the soft and dense tissues of the periodontal complex, as well as disruption of stromal-vascular interactions, which progress from reversible to irreversible disruption of periodontal connective tissue.

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“…Based on the researches of the relationship between some types of microorganisms and destructive periodontal diseases ( 4 , 5 ), two main points of view were formulated regarding the pathogenesis of inflammatory periodontal diseases: the first—there are certain bacterial pathogens that cause destructive damage to periodontal tissues; the second—the development of generalized periodontitis is caused by an imbalance of the body's protective and adaptive mechanisms ( 6 ). If we follow only the microbial etiology of periodontitis ( 7 ), the development of this disease requires a combination of the following conditions: the presence of periodontopathogenic bacteria in a quantity sufficient to initiate the inflammatory process; living conditions in the oral cavity should contribute to the growth and reproduction of pathogenic microbiota ( 8 ). Periodontal tissues must be free of microorganisms—antagonists of periodontopathogenic bacteria; microorganisms must be spatially localized so that they and (or) the products of their vital activity can act directly on target cells; the human body must be sensitive to microbes and their toxins.…”

Section: Introductionmentioning

confidence: 99%

The influence of microbiota on the development and course of inflammatory diseases of periodontal tissues

Demkovych,

Kalashnikov,

Hasiuk

et al. 2023

Front. Oral. Health

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An important feature of the functioning of the organs and tissues of the oral cavity is the fact that all processes that take place in it are carried out in the constant presence of various microorganisms that cause the development of pathological processes in the body or are associated with them. In the pathogenesis of chronic generalized periodontitis, dental plaque penetrates the bottom of the gingival sulcus, penetrating under the epithelium into the stroma of the connective tissue, causing its inflammation. Bacteria produce a number of toxic substances that have a toxic effect on surrounding tissues. Most bacteria produce chain fatty acids that inhibit chemotaxis of leukocytes and phagocytes. Anaerobes and spirochetes secrete a number of substances (propionic acid and indole) that are extremely toxic to most tissues. Inflammation in the periodontal tissues is caused by the microbiota of the dental plaque biofilm. As periodontitis develops, an increase in the number of P. gingivalis, P. intermedia and T. forsythia was found in it, more than 100 times. Therefore, the given data prove that in the development and course of the inflammatory process in the periodontal tissues, complex dysbiotic and tissue-cellular interactions are involved, the dynamic balance of which depends on its outcome.

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State of collagenolysis in experimental periodontitis of bacterial-immune genesis and its correction with flavonol

Cited by 15 publications

References 0 publications

Features of Changes in Indicators of Local Immunity of the Oral Cavity and Blood Serum of Patients Against the Background of Generalized Periodontitis and Type 2 Diabetes Mellitus

Features of Changes in Indicators of Local Immunity of the Oral Cavity and Blood Serum of Patients Against the Background of Generalized Periodontitis and Type 2 Diabetes Mellitus

Morphological Changes of Periodontal Components Under Experimental Lipopolysaccharide Periodontitis Combined With Hyperthyroidism

The influence of microbiota on the development and course of inflammatory diseases of periodontal tissues

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