The article presents an assessment of the dynamics of changes in the content of the marker of collagenolysis – free oxyproline in the homogeniate of soft tissues and bone in experimental bacterial-immune periodontitis and elucidation of the effect of flavonol quercetin on these indicators. The aim of this study was to determine the role of cytokinogenesis and the effect of flavonol on it in the pathogenesis, development and course of experimental periodontitis. During the experiment, a fragment of the mandible was taken from the animals, from which the soft tissues and bone were carefully separated. The state of collagen was determined by the content of free oxyproline in the soft and bone tissues. The concentration was determined according to the calibration graph and expressed in μmol/g. The results of studies of the indicators of the state of biopolymers of connective tissue structures of periodontium on the 7th, 14th and 30th day of experimental bacterial-immune periodontitis and after its correction with flavonol (from the 7th to the 14th day of the experiment) are presented. The data on the nature of changes in the content of collagen monomers in the process of formation of the inflammatory focus in the periodontal complex are given. During the acute phase of the inflammatory process in rats there was revealed a slight increase in blood free oxyproline in bone homogenate and homogenate of soft periodontal tissues, on the 14th day the dynamics continued to increase, at a later stage of the experiment, namely on the 30th day, increase in bone resorption continued  as compared to the 7th and 14th day. During the correction of disorders resulted from  the development of this pathological process there was a decrease in the level of free oxyproline in the bone homogenate and homogenate of soft tissues of mandibular periodontium, as compared to the same indicators of animals who did not receive quercetin on the 14th day. The use of flavonol quercetin, which, by affecting immune processes, limited the inflammatory response in periodontal tissues and stabilized collagenolysis processes in periodontal tissues was manifested by a decrease in free oxyproline in bone and soft tissue homogenates of experimental animals.

No abstract

The results of biochemical studies of the antioxidant preventive system are given in the article. They were determined by the activity of superoxide dismutase (SOD), catalase, ceruloplasmin, glutathione peroxidase, glutathione reductase and reduced glutathione on the 7 th , 14 th and 30 th days of development of experimental periodontitis. It is shown that the generation of reactive oxygen species is associated with an increase in the activity of immune processes, which becomes more potent and provides a protracted character to the inflammatory process in the periodontal tissues. In that is paid attention to the characteristic dynamics of changes in the activity indices of lipid peroxidative processes and the antioxidant protective system in the development of experimental periodontitis. In the early period of development of experimental periodontitis, that is, on the 7 th day, the activity of SOD in the blood serum decreased, and later, on the 14 th and 30 th days, it increased. On the 7 th day of the experiment, an increase of the catalase activity and ceruloplasmin in the blood serum was found, respectively) in comparison with the control. On the 14 th day catalase activity and ceruloplasmin was decreased as compared to the group of animals examined on the 7 th day of the experiment. On the 30 th day their levels in the blood serum still is remained decreased. The dynamics of indices reduced glutathione were similar to the dynamics of superoxide dismutase activity, that is, on the 7 th day of the experiment there was a decrease. The partial increase enzymic activity of the glutathione system (glutathione peroxidase, glutathione reductase) occurred on the 14 th day of the inflammation but subsequently these parameters became the opposite of changes. It is established that in the conditions of formation and course of experimental periodontitis the intensity of antioxidant protection activity varies.

No abstract

Background. The change in metabolism of the connective tissue elements of heart is the central chain in pathogenesis of diffuse ischemic necrotic cardiosclerosis (DINC),