2008
DOI: 10.1002/bdrb.20158
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State of the art in developmental toxicity screening methods and a way forward: a meeting report addressing embryonic stem cells, whole embryo culture, and zebrafish

Abstract: A meeting was convened so that users of three models for in vitro developmental toxicity (embryonic stem cells, whole embryo culture, and zebrafish) could share their experiences with each model, and explore the areas for improvement. We present a summary of this meeting and the recommendations of the group.

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Cited by 69 publications
(29 citation statements)
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“…Thus embryonic stem cells themselves model the very young embryo, while embryonic stem cells, which differentiate, model later stages of prenatal development. The most thoroughly developed model based on embryonic stem cells and the only one that has been validated is the "embryonic stem cell test" developed in Europe (Chapin et al, 2008;Croxatto & Villalón, 1994;Dávila et al, 2004). While originally based on differentiation of contracting cardiomyocytes, this assay has undergone continual improvements to include molecular endpoints (Seiler et al, 2004;zur Nieden, Kempka & Ahr, 2004) and also to reduce the time required to perform the assay (Buesen et al, 2009).…”
Section: Studies Using Embryonic Stem Cells (Esc)mentioning
confidence: 99%
“…Thus embryonic stem cells themselves model the very young embryo, while embryonic stem cells, which differentiate, model later stages of prenatal development. The most thoroughly developed model based on embryonic stem cells and the only one that has been validated is the "embryonic stem cell test" developed in Europe (Chapin et al, 2008;Croxatto & Villalón, 1994;Dávila et al, 2004). While originally based on differentiation of contracting cardiomyocytes, this assay has undergone continual improvements to include molecular endpoints (Seiler et al, 2004;zur Nieden, Kempka & Ahr, 2004) and also to reduce the time required to perform the assay (Buesen et al, 2009).…”
Section: Studies Using Embryonic Stem Cells (Esc)mentioning
confidence: 99%
“…However, modifications to the test continue to be suggested. Various recommended changes can be found in Chapin et al [2008] and MarxStoelting et al [2009]. The recommendations include alterations in the evaluation of the endpoint as well as inclusion of other cell lineages.…”
Section: Mouse Embryonic Stem Cell Test (Mest)mentioning
confidence: 99%
“…92521, USA; Tel: 951-827-3768; Fax: 951-827-4286; E-mail: talbot@ucr.edu non-mammalian species such as the zebrafish [3]. The cost of performing animal studies is very high, requires a large number of animals for reliable data, often requires a significant amount of time to complete, and is based on non-human species that may not respond the same way to a test chemical as humans.…”
Section: Introductionmentioning
confidence: 99%
“…The value of using in vitro models for measuring toxicity of environmental chemicals and drugs has been appreciated for many years and discussed in recent reviews [3][4]. In contrast to laborious in vivo models that require many animals and may not be accurate predictors for humans, in vitro models potentially enable high throughput screening of chemicals and drugs and can be done using human cells, which should be better predictors of human health effects than models based on non-human species.…”
Section: Introductionmentioning
confidence: 99%
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