State of the art in integrated biosensors for organ-on-a-chip applications

Zhu, Yangzhi; Mandal, Kalpana; Hernández, Ana López; Kawakita, Satoru; Huang, Wei; Bandaru, Praveen; Ahadian, Samad; Kim, Hanjun; Jucaud, Vadim; Dokmeci, Mehmet R.; Khademhosseini, Ali

doi:10.1016/j.cobme.2021.100309

Cited by 51 publications

(43 citation statements)

References 38 publications

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“…Additional challenges biosensors incorporation in MPSs are scalability, biofouling, and reliability that should be addressed. [ 234 ] The combination of active materials and off‐chip detection systems that exclude the use of invasive probes could be a viable option. For example, nitrogen‐vacancy centers (NV) in diamonds are used for noninvasive and noncontact broadband vector magnetic field sensing and imaging.…”

Section: Discussionmentioning

confidence: 99%

Advanced Materials and Sensors for Microphysiological Systems: Focus on Electronic and Electrooptical Interfaces

2022

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Advanced in vitro cell culture systems or microphysiological systems (MPSs), including microfluidic organ‐on‐a‐chip (OoC), are breakthrough technologies in biomedicine. These systems recapitulate features of human tissues outside of the body. They are increasingly being used to study the functionality of different organs for applications such as drug evolutions, disease modeling, and precision medicine. Currently, developers and endpoint users of these in vitro models promote how they can replace animal models or even be a better ethically neutral and humanized alternative to study pathology, physiology, and pharmacology. Although reported models show a remarkable physiological structure and function compared to the conventional 2D cell culture, they are almost exclusively based on standard passive polymers or glass with none or minimal real‐time stimuli and readout capacity. The next technology leap in reproducing in vivo‐like functionality and real‐time monitoring of tissue function could be realized with advanced functional materials and devices. This review describes the currently reported electronic and optical advanced materials for sensing and stimulation of MPS models. In addition, an overview of multi‐sensing for Body‐on‐Chip platforms is given. Finally, one gives the perspective on how advanced functional materials could be integrated into in vitro systems to precisely mimic human physiology.

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Section: Discussionmentioning

confidence: 99%

Advanced Materials and Sensors for Microphysiological Systems: Focus on Electronic and Electrooptical Interfaces

2022

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“…[113,114] To enable in-line monitoring of adipose tissue secretions, on-chip sensors based on different technologies could be integrated. [44,46,115,116] To set up the WAT model, we established protocols and processes that allow to source all the different cell types from one individual donor; more specifically, from tissue samples of subcutaneous adipose tissue with skin that are readily available in most hospitals. The choice of a human cell source is an integral part for human-centered research.…”

Section: Discussionmentioning

confidence: 99%

“…[ 113 , 114 ] To enable in‐line monitoring of adipose tissue secretions, on‐chip sensors based on different technologies could be integrated. [ 44 , 46 , 115 , 116 ]…”

Section: Discussionmentioning

confidence: 99%

Autologous Human Immunocompetent White Adipose Tissue‐on‐Chip

et al. 2022

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Obesity and associated diseases, such as diabetes, have reached epidemic proportions globally. In this era of "diabesity", white adipose tissue (WAT) has become a target of high interest for therapeutic strategies. To gain insights into mechanisms of adipose (patho-)physiology, researchers traditionally relied on animal models. Leveraging Organ-on-Chip technology, a microphysiological in vitro model of human WAT is introduced: a tailored microfluidic platform featuring vasculature-like perfusion that integrates 3D tissues comprising all major WAT-associated cellular components (mature adipocytes, organotypic endothelial barriers, stromovascular cells including adipose tissue macrophages) in an autologous manner and recapitulates pivotal WAT functions, such as energy storage and mobilization as well as endocrine and immunomodulatory activities. A precisely controllable bottom-up approach enables the generation of a multitude of replicates per donor circumventing inter-donor variability issues and paving the way for personalized medicine. Moreover, it allows to adjust the model's degree of complexity via a flexible mix-and-match approach. This WAT-on-Chip system constitutes the first human-based, autologous, and immunocompetent in vitro adipose tissue model that recapitulates almost full tissue heterogeneity and can become a powerful tool for human-relevant research in the field of metabolism and its associated diseases as well as for compound testing and personalized-and precision medicine applications.

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“…In the future, technological refinements could aim at scale-up and sensor integration: To achieve parallelized platforms with increased throughput, a combination with enabling technologies might be worthwhile; e.g., the WAT-on-chip design could be transferred to the organ-on-disc platform (Schneider et al, 2020;Stefan Schneider et al, 2021). To enable in-line monitoring of adipose tissue secretions, on-chip sensors based on different technologies could be integrated (Fuchs et al, 2021;Hu et al, 2020;Zhu et al, 2018Zhu et al, , 2021.…”

Section: Discussionmentioning

confidence: 99%

Autologous human immunocompetent white adipose tissue-on-chip

Rogal

Roosz

et al. 2021

Preprint

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Obesity and associated diseases, such as diabetes, have reached epidemic proportions globally. In the era of 'diabesity' and due to its central role for metabolic and endocrine processes, adipose tissue (specifically white adipose tissue; WAT) has become a target of high interest for therapeutic strategies. To gain insights in cellular and molecular mechanisms of adipose (patho-)physiology, researchers traditionally relied on animal models since in vitro studies on human WAT are challenging due to the large size, buoyancy, and fragility of mature white adipocytes. Leveraging the Organ-on-Chip technology, we introduce a next-generation microphysiological in vitro model of human WAT based on a tailored microfluidic platform featuring vasculature-like perfusion. The platform integrates a 3D tissue comprising all major WAT-associated cellular components in an autologous manner, including not only mature adipocytes but also organotypic endothelial barriers and stromovascular cells featuring tissue-resident innate immune cells, specifically adipose tissue macrophages. This microphysiological tissue model recapitulates pivotal WAT functions, such as energy storage and mobilization as well as endocrine and immunomodulatory activities. The combination of all individual cell types with extra cellular matrix-like hydrogels in a precisely controllable bottom-up approach enables the generation of a multitude of replicates from the same donors circumventing issues of inter-donor variability and paving the way for personalized medicine. Moreover, it allows to adjust the model's degree of complexity to fit a specific purpose via a flexible mix-and-match approach with different cell component modules. This novel WAT-on-chip system constitutes a human- based, autologous and immunocompetent in vitro model of adipose tissue that recapitulates almost full tissue heterogeneity. In the future, the new WAT-on-chip model can become a powerful tool for human-relevant research in the field of metabolism and its associated diseases as well as for compound testing and personalized- and precision medicine applications.

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State of the art in integrated biosensors for organ-on-a-chip applications

Cited by 51 publications

References 38 publications

Advanced Materials and Sensors for Microphysiological Systems: Focus on Electronic and Electrooptical Interfaces

Advanced Materials and Sensors for Microphysiological Systems: Focus on Electronic and Electrooptical Interfaces

Autologous Human Immunocompetent White Adipose Tissue‐on‐Chip

Autologous human immunocompetent white adipose tissue-on-chip

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