Static and dynamic imaging of alveoli using optical coherence tomography needle probes

McLaughlin, Robert A.; Yang, Xiaojie; Quirk, Bryden C.; Lorenser, Dirk; Kirk, Rodney W.; Noble, Peter B.; Sampson, David D.

doi:10.1152/japplphysiol.00051.2012

Cited by 50 publications

(59 citation statements)

References 61 publications

(60 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…24 OCT imaging through core needles (18 Ga), using fast scanning mechanisms, and probe protection that minimizes tissue disruption has been published before. 25 Therefore, OCT imaging may also be considered for guiding biopsies. However, the probe would be more complex, imaging would require a longer acquisition time, and real-time data analysis would require the use of fast parallel processing approaches.…”

Section: Discussionmentioning

confidence: 99%

Low coherence interferometry approach for aiding fine needle aspiration biopsies

et al. 2014

View full text Add to dashboard Cite

Abstract. We present portable preclinical low-coherence interference (LCI) instrumentation for aiding fine needle aspiration biopsies featuring the second-generation LCI-based biopsy probe and an improved scoring algorithm for tissue differentiation. Our instrument and algorithm were tested on 38 mice with cultured tumor mass and we show the specificity, sensitivity, and positive predictive value of tumor detection of over 0.89, 0.88, and 0.96, respectively.

show abstract

Section: Discussionmentioning

confidence: 99%

Low coherence interferometry approach for aiding fine needle aspiration biopsies

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Dynamic OCT scans of lungs undergoing simulated respiration have previously been demonstrated in [13], producing a sequence of lung scans at different stages of alveolar expansion and recruitment. Alternatively, Williamson et al [47] demonstrated the acquisition of a series of OCT scans at predefined lung pressures.…”

Section: Discussionmentioning

confidence: 99%

“…Tissue was obtained from animals sacrificed as part of a different research project approved by The University of Western Australia Animal Ethics Committee, and utilized under institutional tissue sharing protocols. Two animals were used in this research: an adult rat, and an adult pig, and imaged according to the protocol described in [13]. The animals were euthanized on the day of the experiment, and dissection was performed immediately post-mortem.…”

Section: Tissue Preparationmentioning

confidence: 99%

“…Both the rat and pig lungs were allowed to deflate passively for 1 to 2 hours after excision. After tracheal cannulation, the lungs were inflated by a syringe filled with isotonic saline to improve image quality and minimize imaging artifacts that arise from the refractive index mismatch between the air-filled alveoli and the tissue of the parenchyma, as described in [13].…”

Section: Tissue Preparationmentioning

confidence: 99%

“…However, OCT has a limited penetration depth in tissue of 1-2 mm, and so has been restricted to imaging peripheral tissue [9][10][11]. Recent work has demonstrated the ability to image structures such as alveoli situated more deeply in the lung using an OCT needle probe [13,14]. This is achieved by encasing the miniaturized imaging optics within a hypodermic needle.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Automated quantification of lung structures from optical coherence tomography images

Pagnozzi

Kirk

Kennedy

et al. 2013

Biomed. Opt. Express

Self Cite

View full text Add to dashboard Cite

Abstract:Characterization of the size of lung structures can aid in the assessment of a range of respiratory diseases. In this paper, we present a fully automated segmentation and quantification algorithm for the delineation of large numbers of lung structures in optical coherence tomography images, and the characterization of their size using the stereological measure of median chord length. We demonstrate this algorithm on scans acquired with OCT needle probes in fresh, ex vivo tissues from two healthy animal models: pig and rat. Automatically computed estimates of lung structure size were validated against manual measures. In addition, we present 3D visualizations of the lung structures using the segmentation calculated for each data set. This method has the potential to provide an in vivo indicator of structural remodeling caused by a range of respiratory diseases, including chronic obstructive pulmonary disease and pulmonary fibrosis. References and links1. G. Turato, R. Zuin, and M. Saetta, "Pathogenesis and pathology of COPD," Respiration 68(2), 117-128 (2001). 2. T. C. Kravis, A. Ahmed, T. E. Brown, J. D. Fulmer, and R. G. Crystal, "Pathogenic mechanisms in pulmonary fibrosis: collagen-induced migration inhibition factor production and cytotoxicity mediated by lymphocytes," J. Clin. Invest. 58(5), 1223-1232 (1976

show abstract

Numerical Analysis of Optical Fiber Refractive Index in the Miniaturized Graded‐Index Fiber Probe

Bi,

Zhang,

Tang

et al. 2024

Engineering Reports

View full text Add to dashboard Cite

We analyze the impact of the refractive index of optical fibers on the focusing properties of a miniaturized graded‐index (GRIN) fiber probe. The ABCD ray transfer matrix and characteristic parameters are employed to characterize the working distance, focusing spot size, and the depth of field effectively. The three‐dimensional (3‐D) function diagrams and two‐dimensional (2‐D) graphs are used to illustrate the impact of the no‐core fiber (NCF) refractive index and the center refractive index of GRIN fiber on the focusing properties. Numerical analysis results show that when the length of the NCF is 0.36 mm and the GRIN fiber is 0.1 mm, the variation of the refractive index of the NCF between 1.44 and 1.52 leads to the variation of the working distance in the range of 0.02 mm, while the focusing spot size varies in the range of 5 μm. A comparison of 12 probe samples reveals that a 0.02 difference in the center refractive index of GRIN fiber could result in a 0.16 mm variation in working distance and a variation in focusing spot size of over 3 μm. The experimental results indicate that alterations in the length of the fibers have a considerable effect on the focusing properties of the probe. In contrast, the range of variation in focusing properties with fiber refractive index is relatively limited.

show abstract

Static and dynamic imaging of alveoli using optical coherence tomography needle probes

Abstract: McLaughlin RA, Yang X, Quirk BC, Lorenser D, Kirk RW, Noble PB, Sampson DD. Static and dynamic imaging of alveoli using optical coherence tomography needle probes.

Cited by 50 publications

References 61 publications

Low coherence interferometry approach for aiding fine needle aspiration biopsies

Low coherence interferometry approach for aiding fine needle aspiration biopsies

Automated quantification of lung structures from optical coherence tomography images

Numerical Analysis of Optical Fiber Refractive Index in the Miniaturized Graded‐Index Fiber Probe

Contact Info

Product

Resources

About